温度及外源CaCO<sub>3</sub>对砂滤微生物氨氮降解的影响特性

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Abstract In this paper, the ammonia degradation performance and variations of microbial community composition of the attached microbes in waterworks sand filters under different temperatures (10, 15, 25 and 37℃), ammonia concentrations (1 and 2.5mg-N/L) and exogenous addition of CaCO₃ were investigated. The nitrifiers were successfully extracted from the sand filter in the waterworks, which included ammonia oxidizer, nitrite oxidizer and complete ammonia oxidizer as characterized by PCR- electrophoresis. The results of temperature influenced test showed that: the ammonia oxidation rate notably decreased by 48.1% and 47.3%, respectively under 1 and 2.5mg-N/L ammonia concentrations when the temperature decreased from 25℃ to 10℃. At 10℃, the system displayed high total nitrogen removal capacity (~48.1%), but the nitrite oxidation activity was significantly inhibited, and the increase of ammonia nitrogen concentrations exerted adverse effects on the nitrite oxidation. In contrast, the temperature as high as 37℃ caused the complete loss of the nitrification performance. Moreover, exogenous addition of CaCO₃ significantly improved the ammonia oxidation rate by more than 90%. High-throughput sequencing results showed that a decrease in temperature (10℃ and 15℃) significantly inhibited the growth of Nitrosospira and Nitrosomonas (both relative abundance < 1%). When the temperature decreased to 10~15℃, Nitrospira outcompeted the other nitrifiers with the relative abundances increased by 63.3%-113.7%. Besides, the addition of CaCO₃ caused a 2.5-fold increase in the relative abundance of Nitrospira, while the relative abundances of the other nitrifiers were all less than 1.0%. Therefore, it is inferred that the complete ammonia oxidation Nitrospira under cold condition had a high contribution to ammonia degradation.

Key words： nitrifying bacteria temperature complete ammonia oxidation high-throughput sequencing

Received: 14 January 2023

PACS:

X172

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	LIU Ming-hui
	DING Cheng-long
	ZHU Min
	LI Yi
	WU Jun-kang
	JING Zhao-qian
	YU Ran

Cite this article:

LIU Ming-hui,DING Cheng-long,ZHU Min等. Effects of temperature and exogenous CaCO₃ on the microbial degradation of ammonia nitrogen in waterworks sand filters[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(9): 4896-4904.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I9/4896

[1]	中华人民共和国生态环境部.2020中国生态环境状况公报[R]. 2021.32-33. Ministry of Ecology and Environment of the People's Republic of China. 2020 Report on the state of the environment in China[R]. 2021.32-33.
[2]	李奕燃,董昆,于雪,等.利16S rRNA高通量测序技术考察温度对生物脱氮硝化过程中亚硝酸盐氧化菌代谢功能的影响[J]. 环境工程学报, 2022,16(3):980-988. Li Y R, Dong K, Yu X, et al. Investigation on the effect of temperature on the metabolism and functionof nitrite-oxidizing bacteria in the process of biological nitrification by 16S rRNA high-throughput sequencing technology[J]. Chinese Journal of Environmental Engineering, 2022,16(3):980-988.
[3]	于莉芳,傅学焘,杨秀玲,等.2种温度下污泥水富集硝化菌的对比[J]. 环境工程学报, 2018,12(1):102. Yu L F, Fu X T, Yang X L, et al. Comparison of nitrifier accumulation in sludge liquor treatment system at two different temperatures[J]. Chinese Journal of Environmental Engineering, 2018,12(1):102.
[4]	He S, Ding L, Li K, et al. Comparative study of activated sludge with different individual nitrogen sources at a low temperature:Effluent dissolved organic nitrogen compositions, metagenomic and microbial community[J]. Bioresource Technology, 2018,247:915.
[5]	Costa E, Perez J, Kreft J U. Why is metabolic labour divided in nitrification?[J]. Trends in Microbiology, 2006, 14(5):213.
[6]	Hu H W, He J Z. Comammox-a newly discovered nitrification process in the terrestrial nitrogen cycle[J]. Journal of Soils Sediments, 2017, 17(12):2709.
[7]	Kessel M V, Speth D R, Albertsen M, et al. Complete nitrification by a single microorganism[J]. Nature, 2015,528(7583).
[8]	Pollard P C. A quantitative measure of nitrifying bacterial growth[J]. Water Research, 2006,40(8):1569.
[9]	Ibrahim RS, Sabeen A H, Noor Z Z, et al. Comparative study of suspended and attached growth in membrane bioreactors for wastewater treatment[J]. Water and Environment Journal, 2020,34:273.
[10]	Kangwannarakul N, Wantawin C, Noophan P. Anammox bacteria with attached-growth media for nitrogen removal in wastewater[J]. Clean Technologies and Environmental Policy, 2018,20(1):219.
[11]	Robertson J, McGoverin C, White J R, et al. Rapid detection of escherichia coli antibiotic susceptibility using live/dead spectrometry for lytic agents[J]. Microorganisms, 2021,9(5):27.
[12]	Hu W, Murata K, Zhang D Z. Applicability of LIVE/DEAD baclight stain with glutaraldehyde fixation for the measurement of bacterial abundance and viability in rainwater[J]. Journal of Environmental Sciences, 2017,51:202.
[13]	Ferris M J, Muyzer G, Ward D M. Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community[J]. Applied and Environmental Microbiology, 1996,62(2):340.
[14]	Rotthauwe J H, Witzel K P, Liesack W. The ammonia monooxygenase structural gene amoA as a functional marker:molecular fine-scale analysis of natural ammonia-oxidizing populations[J]. Applied and Environmental Microbiology, 1997,63(12):4704.
[15]	Graham D W, Knapp C W, Van Vleck E S, et al. Experimental demonstration of chaotic instability in biological nitrification[J]. Isme Journal, 2007,1(5):385.
[16]	Pester M, Maixner F, Berry D, et al. NxrB encoding the beta subunit of nitrite oxidoreductase as functional and phylogenetic marker for nitrite-oxidizing Nitrospira[J]. Environmental Microbiology, 2014, 16(10):3055.
[17]	Kits K D, Jung M Y, Vierheilig J, et al. Low yield and abiotic origin of N2O formed by the complete nitrifier Nitrospira inopinata[J]. Nature Communications, 2019,10(1):1836.
[18]	Holm-Hansen C. A salicylate-hypochlorite method for determining ammonia in seawater[J]. Journal Canadien Des Sciences Halieutiques Et Aquatiques, 1980,37(5):794.
[19]	Garcia-Robledo E, Corzo A, Papaspyrou S. A fast and direct spectrophotometric method for the sequential determination of nitrate and nitrite at low concentrations in small volumes[J]. Marine Chemistry, 2014,162(may 20):30-36.
[20]	Pjevac P, Schauberger C, Poghosyan L, et al. AmoA-targeted polymerase chain reaction primers for the specific detection and quantification of Comammox Nitrospira in the environment[J]. Frontiers in Microbiology, 2017,(8):1508.
[21]	Wang Y, Ma L, Mao Y, et al. Comammox in drinking water systems[J]. Water Research, 2017,116(Jun.1):332.
[22]	Zhou X, Li B L, Wei J, et al. Temperature influenced the Comammox community composition in drinking water and wastewater treatment plants[J]. Microbial Ecology, 2021:1-15.
[23]	梁东博,卞伟,阚睿哲,等.不同温度下应用比值控制实现连续流好氧颗粒污泥短程硝化[J]. 环境科学, 2018,39(4):1713. Liang D B, Bian W, Kan R Z, et al. Achieving partial nitritation in a continuous-flow aerobic granular sludge reactor at different temperatures through ratio control.[J]. Environmental Science, 2018, 39(4):1713.
[24]	刘宏,彭永臻,卢炯元,等.温度对间歇曝气SBR短程硝化及硝化活性的影响[J]. 环境科学, 2017,38(11):4656. Liu H, Peng Y Z, Lu J, et al. Effects of temperature on shortcut nitrification and nitrification activity of nitrification in an intermittent aeration sequencing batch reactor[J]. Environmental Science, 2017, 38(11):4656.
[25]	D'Aoust P M, Vincent S, LeBlond G, et al. Upgrading municipal lagoons in temperate and cold climates:Total nitrogen removal and phosphorus assimilation at ultra-low temperatures[J]. Water and Environment Journal, 2022,36(2):308.
[26]	Zheng K K, Li H B, Wang S, et al. Enhanced proteins and amino acids production based on ammonia nitrogen assimilation and sludge increment by the integration of bioadsorption with anaerobic-anoxic-oxic (AAO) process[J]. Chemosphere, 2021,(280):130721.
[27]	Wang G J, Tang Z K, Wei J, et al. Effect of salinity on anammox nitrogen removal efficiency and sludge properties at low temperature[J]. Environmental Technology, 2020,41(22):2920.
[28]	Wang L, Zhu J, Miller C. The stability of accumulating nitrite from swine wastewater in a sequencing batch reactor[J]. Applied Biochemistry and Biotechnology, 2011,163(3):362.
[29]	Xu L, Zhang B, Peng X, et al. Dynamic variations of microbial community structure in Myriophyllum aquaticum constructed wetlands in response to different NH4+-N concentrations[J]. Process Biochemistry, 2020,93:55.
[30]	许艳俊,李静媛. pH和Ca2+协同作用对酵母代谢及细胞膜功能的影响[J]. 生物技术通报, 2018,34(3):208. Xu Y J, Li J Y. Synergetic effects of pH and Ca2+ on yeast metabolism and cell membrane function[J]. Biotechnology Bulletin, 2018,34(3):208.
[31]	Vijayan A, Vattiringal Jayadradhan R K, Pillai D, et al. Nitrospira as versatile nitrifiers:Taxonomy, ecophysiology, genome characteristics, growth, and metabolic diversity[J]. Journal of Basic Microbiology, 2021,(2):61.
[32]	辛佳期.钙离子对藻菌-膜组合工艺处理养殖废水膜污染机制的影响[D]. 南昌:南昌大学, 2021. Xin J Q. Effect of Calcium ion on the membrane fouling mechanism of algalbacteria symbiosis-membrane process for treatment of livestock wastewater[D]. Nanchang:Nanchang University, 2021.
[33]	Cotto I, Dai Z H, Huo L X, et al. Long solids retention times and attached growth phase favor prevalence of comammox bacteria in nitrogen removal systems[J]. Water Research, 2020,(169):115268.
[34]	Zhang Q, Yang Y, Chen F, et al. Effects of hydraulic loading rate and substrate on ammonium removal in tidal flow constructed wetlands treating black and odorous water bodies[J]. Bioresource Technology, 2021,(321):124468.
[35]	Han P, Yu Y, Zhou L, et al. Specific micropollutant biotransformation pattern by the comammox bacterium Nitrospira inopinata[J]. Environmental Science and Technology, 2019,53(15):8695.
[36]	Poghosyan L, Koch H, Frank J, et al. Metagenomic profiling of ammonia-and methane-oxidizing microorganisms in two sequential rapid sand filters[J]. Water Research, 2020,(185):116288.
[37]	Kits K D, Sedlacek C J, Lebedeva E V, et al. Kinetic analysis of a complete nitrifier reveals an oligotrophic lifestyle[J]. Nature, 2017,549(7671):269.
[38]	王永刚.多组学技术解析红平红球菌KB1低温降解石油烃的遗传学基础及应用[D]. 兰州:兰州理工大学, 2021. Wang Y G. Genetic basis analysis and application of Rhodococcus erythropolis KB1 with the ability on petroleum hydrocarbons degradation at low temperature using multiple omics techniques[D]. Lanzhou:Lanzhou University of Technology, 2021.
[39]	Zhang H, Shi Y, Huang T, et al. NirS-type denitrifying bacteria in aerobic water layers of two drinking water reservoirs:Insights into the abundance, community diversity and co-existence model[J]. Journal of Environmental Sciences, 2023,(124):215.
[40]	Kanehisa M, Furumichi M, Tanabe M, et al. KEGG:New perspectives on genomes, pathways, diseases and drugs[J]. Nucleic Acids Research, 2017,(D1):D353-D361.