风化煤固定化微生物细胞制备及其固定化机制

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Abstract In this study, the modified weathered coal was used as the carrier to prepare for immobilized microbial cells by adsorption immobilization method. PBD (Plackett-Burman) and PBB (Box-Behnken) were used to optimize the immobilization process of microbial cells, so that the ability of this cells was maximized to passivate lead polluted water. A variety of characterization techniques were used to study the binding characteristics of microbial cells and carriers. These results showed that the quantity of microbial cells, immobilized temperature and pH were the key factors. When inoculation quantity was 7, the immobilized temperature was 31℃, and pH was 6.5, the unit Pb²⁺ adsorption capacity and removal rate of immobilized microbial cells could reach 313.83mg/g and 80.42%, which were 3.8times higher than free microbial cells. Compared with similar materials at home and abroad, it has a high level. Surface adsorption, pore filling, electrostatic attraction and covalent bonds (-OH, -NH₂, -CONH₂, -COOR) promote the immobilization of microorganisms on weathered coal jointly. This study can provide theoretical support for the preparation of immobilized microbial cells by weathered coal carriers.

Key words： weathered coal microbial cells immobilization PBD PBB

Received: 09 June 2023

PACS:

X172

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	Articles by authors
	JIAO Zi-le
	LI Jian-hua
	CHEN Xiao-jing
	LU Jin-jing
	GAO Chun-hua
	XU Ming-gang

Cite this article:

JIAO Zi-le,LI Jian-hua,CHEN Xiao-jing等. Preparation and immobilization mechanism of weathered coal-immobilized microbial cells[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(9): 4905-4915.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I9/4905

[1]	Tang J L, Chen Y B, Zhao M H, et al. Phenylthiosemicarbazide-functionalized UiO-66-NH2 as highly efficient adsorbent for the selective removal of lead from aqueous solutions[J]. Journal of Hazardous Materials, 2021,413:125278.
[2]	Tran H N, Nguyen D T, Le G T, et al. Adsorption mechanism of hexavalent chromium onto layered double hydroxides-based adsorbents:A systematic in-depth review[J]. Journal of Hazardous Materials, 2019,373:258-270.
[3]	Li Y R, Bai P, Yan Y, et al. Removal of Zn2+, Pb2+, Cd2+, and Cu2+ from aqueous solution by synthetic clinoptilolite[J]. Microporous and Mesoporous Materials, 2019,273:203-211.
[4]	Xu D M, Fu R B, Liu H Q, et al. Current knowledge from heavy metal pollution in Chinese smelter contaminated soils, health risk implications and associated remediation progress in recent decades:A critical review[J]. Journal of Cleaner Production, 2021,286:124989.
[5]	Samimi M, Shahriari-Moghadam M. Isolation and identification of Delftia lacustris Strain-MS3as a novel and efficient adsorbent for lead biosorption:Kinetics and thermodynamic studies, optimization of operating variables[J]. Biochemical Engineering Journal, 2021,173:108091.
[6]	Mu X, Wang Z X, Liu L C, et al. Multiple exposure pathways of first-year university students to heavy metals in China:Serum sampling and atmospheric modeling[J]. Science of the Total Environment, 2020,746:141405.
[7]	Gao L, Li Z H, Yi W M, et al. Impacts of pyrolysis temperature on lead adsorption by cotton stalk-derived biochar and related mechanisms[J]. Journal of Environmental Chemical Engineering, 2021,9(4):105602.
[8]	廖晓峰,钟静萍,陈云嫩,等.功能化凹凸棒吸附材料的制备及其对重金属废水中Pb2+的吸附行为[J]. 环境科学, 2022,43(1):387-397. Liao X F, Zhong J P, Chen Y N, et al. Preparation of functional attapulgite composite and its adsorption behaviors for congo red[J]. Environmental Science, 2022,43(1):387-397.
[9]	Meunier N, Drogui P, Montané C, et al. Comparison between electrocoagulation and chemical precipitation for metals removal from acidic soil leachate[J]. Journal of Hazardous Materials, 2006,137(1):581-590.
[10]	Sun Y, Wu Z Y, Wang X, et al. Macroscopic and microscopic investigation of U(VI) and Eu(III) adsorption on carbonaceous nanofibers[J]. Environmental Science & Technology, 2016,50(8):4459-4467.
[11]	Gao J, Sun S P, Zhu W P, et al. Chelating polymer modified P84nanofiltration (NF) hollow fiber membranes for high efficient heavy metal removal[J]. Water Research, 2014,63:252-261.
[12]	Vardhan K H, Kumar P S, Panda R C. A review on heavy metal pollution, toxicity and remedial measures:Current trends and future perspectives[J]. Journal of Molecular Liquids, 2019,290:111197.
[13]	Liu L Q, Huang Y J, Meng Y H, et al. Investigating the adsorption behavior and quantitative contribution of Pb2+ adsorption mechanisms on biochars by different feedstocks from a fluidized bed pyrolysis system[J]. Environmental Research, 2020,187:109609.
[14]	Parmar P, Shukla A, Goswami D, et al. Optimization of cadmium and lead biosorption onto marine Vibrio alginolyticus PBR1employing a Box-Behnken design[J]. Chemical Engineering Journal Advances, 2020,4:100043.
[15]	王雅辉,邹雪刚,舒冉君,等.胡敏素对Pb2+吸附的响应面优化及机理[J]. 中国环境科学, 2017,37(5):1814-1822. Wang Y H, Zou X G, Shu R J, et al. Adsorption of Pb2+ from aqueous solutions by humin:optimization and mechanism[J]. China Environmental Science. 2017,37(5):1814-1822.
[16]	Wen X F, Du C Y, Zeng G M, et al. A novel biosorbent prepared by immobilized Bacillus licheniformis for lead removal from wastewater[J]. Chemosphere, 2018,200:173-179.
[17]	Sohbatzadeh H, Keshtkar A R, Safdari J, et al. U(VI) biosorption by bi-functionalized Pseudomonas putida@chitosan bead:Modeling and optimization using RSM[J]. International Journal of Biological Macromolecules, 2016,89:647-658.
[18]	Bouabidi Z B, El-Naas M H, Zhang Z. Immobilization of microbial cells for the biotreatment of wastewater:A review[J]. Environmental Chemistry Letters, 2019,17(1):241-257.
[19]	Wu P, Wang Z Y, Bhatnagar A, et al. Microorganisms-carbonaceous materials immobilized complexes:Synthesis, adaptability and environmental applications[J]. Journal of Hazardous Materials, 2021, 416:125915.
[20]	Li B B, Gan L, Owens G, et al. New nano-biomaterials for the removal of malachite green from aqueous solution via a response surface methodology[J]. Water Research, 2018,146:55-66.
[21]	张杰,朱晓丽,尚小清,等.生物炭固定化解磷菌对Pb2+的吸附特性[J]. 环境污染与防治, 2019,41(4):387-392. Zhang Jie, Zhu Xiaoli, Shang Xiaoqing, et al. Adsorption characteristics of Pb2+ on biochar immobilized phosphate-solubilizing bacteria[J]. Environmental Pollution and Prevention, 2019,41(4):387-392.
[22]	Zhang W, Shen J N, Zhang H F, et al. Efficient nitrate removal by Pseudomonas mendocina GL6immobilized on biochar[J]. Bioresource Technology, 2021,320(Pt A):124324.
[23]	Huang F, Li K, Wu R, et al. Insight into the Cd2+ biosorption by viable Bacillus cereus RC-1immobilized on different biochars:Roles of bacterial cell and biochar matrix[J]. Journal of Cleaner Production, 2020,272:122743.
[24]	马晓利,武海英,陈平.三乙烯四胺改性风化煤对废水中Ni2+的吸附机制[J]. 环境科学研究, 2018,31(2):337-344. Ma X L, Wu H Y, Chen P. Adsorption of Ni2+ in wastewater by triethylenete-tramine modifued weathered coal[J]. Research of Environmental Sciences, 2018,31(2):337-344.
[25]	An Q, Jin N, Deng S, et al. Ni(II), Cr(VI), Cu(II) and nitrate removal by the co-system of Pseudomonas hibiscicola strain L1 immobilized on peanut shell biochar[J]. Science of The Total Environment, 2022,814:152635.
[26]	孙永鹏,王刚,何扬洋,等.巯基改性小麦秸秆对镉吸附与解吸的性能及机制[J]. 中国环境科学, 2023,43(5):2264-2276. Sun Y P, Wang G, He Y Y, et al. Performance and mechanism of adsorption and desorption for cadmium in aqueous solution with mercaptoacetyl wheat straw[J]. China Environmental Science, 2023, 43(5):2264-2276.
[27]	Plackett R L, Burman J P. The design optimum multifactorial experiments[J]. Biometrika, 1946,33(4):305-325.
[28]	Gao J, Xu H, Li Q J, et al. Optimization of medium for one-step fermentation of inulin extract from Jerusalem artichoke tubers using Paenibacillus polymyxa ZJ-9to produce R,R-2,3-butanediol[J]. Bioresource Technology, 2010,101(18):7076-7082.
[29]	Bera S, Mohanty K. Areca nut (Areca catechu) husks and Luffa (Luffa cylindrica) sponge as microbial immobilization matrices for efficient phenol degradation[J]. Journal of Water Process Engineering, 2020, 33:100999.
[30]	Chen F L, Zhang Q, Fei S M, et al. Optimization of ultrasonic circulating extraction of samara oil from Acer saccharum using combination of Plackett-Burman design and Box-Behnken design[J]. Ultrasonics Sonochemistry, 2017,35:161-175.
[31]	门姝慧,黄占斌,李昉泽,等.黑腐酸对Cd2+的吸附响应面优化及机理研究[J]. 中国环境科学, 2020,40(6):2615-2626. Men S H, Huang Z B, Li F Z, et al. Adsorption of Cd2+ by humin:response surface methodology and mechanism study[J]. China Environmental Science, 2020,40(6):2615-2626.
[32]	Yetilmezsoy K, Demirel S, Vanderbei R J. Response surface modeling of Pb(II) removal from aqueous solution by Pistacia vera L.:Box-Behnken experimental design[J]. Journal of Hazardous Materials, 2009,171(1-3):551-562.
[33]	李璐,杨朝晖,孙珮石,等.基于响应面优化条件下柚皮对Pb2+的吸附[J]. 环境科学学报, 2009,29(7):1426-1433. Li L, Yang C H, Sun P S, et al. Optimiztion of the biosorption Pb2+ by citron peel using response surface methodology[J]. Acta Scientiae Circumstantiae, 2009,29(7):1426-1433.
[34]	李占君,刘运伟,王洪学,等.基于Plackett-Burman和Box-Behnken设计优化超声诱导防风种子发芽方法[J]. 森林工程, 2022,38(1):76-85. Li Z J, Liu Y W, Wang H X, et al. Optimization of ultrasonic induced germination of saposhnikovia divaricate seeds based on Plackett-burman and Box-Behnken design[J]. Forest Engineering, 2022, 38(1):76-85.
[35]	Wang H Y, Gao B, Wang S S, et al. Removal of Pb(II), Cu(II), and Cd(II) from aqueous solutions by biochar derived from KMnO4 treated hickory wood[J]. Bioresource Technology, 2015,197:356-362.
[36]	Sohbatzadeh H, Keshtkar A R, Safdari J, et al. Insights into the biosorption mechanisms of U(VI) by chitosan bead containing bacterial cells:A supplementary approach using desorption eluents, chemical pretreatment and PIXE-RBS analyses[J]. Chemical Engineering Journal, 2017,323:492-501.
[37]	李春全,王丝蒂,汪欣林,等.风化煤基催化材料的制备及其活化PMS降解苯酚[J]. 煤炭学报, 2022,47(5):2067-2077. Li C Q, Wang S D, Wang X L, et al. Preparation of weathered coal-based catalytic material and its performance in phenol degradation by activating peroxymonosulfate[J]. Journal of China Coal Society, 2022,47(5):2067-2077.
[38]	Teng Z D, Shao W, Zhang K Y, et al. Enhanced passivation of lead with immobilized phosphate solubilizing bacteria beads loaded with biochar/nanoscale zero valent iron composite[J]. Journal of Hazardous Materials, 2020,384:121505.
[39]	Samimi M, Shahriari-Moghadam M. Isolation and identification of Delftia lacustris Strain-MS3as a novel and efficient adsorbent for lead biosorption:Kinetics and thermodynamic studies, optimization of operating variables[J]. Biochemical Engineering Journal, 2021,173:108091.
[40]	Wang Y Y, Yao W B, Wang Q W, et al. Synthesis of phosphate-embedded calcium alginate beads for Pb(II) and Cd(II) sorption and immobilization in aqueous solutions[J]. Transactions of Nonferrous Metals Society of China, 2016,26(8):2230-2237.
[41]	Wang N, Qiu Y, Xiao T, et al. Comparative studies on Pb(II) biosorption with three spongy microbe-based biosorbents:High performance, selectivity and application[J]. Journal of Hazardous Materials, 2019,373:39-49.
[42]	Pan X H, Zuo G C, Su T, et al. Polycarboxylic magnetic polydopamine sub-microspheres for effective adsorption of malachite green[J]. Colloids and Surfaces A:Physicochemical and Engineering Aspects, 2019,560:106-113.
[43]	Chen Y, Yu B, Lin J J, et al. Simultaneous adsorption and biodegradation (SAB) of diesel oil using immobilized Acinetobacter venetianus on porous material[J]. Chemical Engineering Journal, 2016,289:463-470.
[44]	Zhang C, Chen Z, Tao Y, et al. Enhanced removal of trichlorfon and Cd(II) from aqueous solution by magnetically separable chitosan beads immobilized Aspergillus sydowii[J]. International Journal of Biological Macromolecules, 2020,148(C).
[45]	Song M, Wei Y X, Cai S P, et al. Study on adsorption properties and mechanism of Pb2+ with different carbon based adsorbents[J]. Science of the Total Environment, 2018,618:1416-1422.
[46]	Bai J, Yang X H, Du R Y, et al. Biosorption mechanisms involved in immobilization of soil Pb by Bacillus subtilis DBM in a multi-metal-contaminated soil[J]. Journal of Environmental Sciences, 2014,26(10):2056-2064.
[47]	陈潇晶,张慧荣,郭彦霞,等.氢氧化钾活化后焦粉的结构变化及其对铅吸附性能的影响[J]. 煤炭转化, 2019,42(6):49-55. Chen X J, Zhang H R, Guo Y X, et al. Structure change of coke after activation by potassium hydroxide and its effect on lead adsorption[J]. Coal Conversion, 2019,42(6):49-55.
[48]	Guo Z, Zhang A, Zhang J, et al. An ammoniation-activation method to prepare activated carbon with enhanced porosity and functionality[J]. Powder Technology, 2017,309:74-78.
[49]	Cui J, Zhang Y, Wang Y, et al. Fabrication of lithium ion imprinted hybrid membranes with antifouling performance for selective recovery of lithium[J]. New Journal of Chemistry, 2017,42(1):118-128.
[50]	Li R, Wang B, Niu A, et al. Application of biochar immobilized microorganisms for pollutants removal from wastewater:A review[J]. Science of the Total Environment, 2022,837:155563.