玛曲高寒退化草地土壤-微生物特征及相互作用

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1745 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract In this paper, four kinds of alpine grassland with different degradation levels in Maqu county, Gannan Tibetan Autonomous Prefecture, Gansu Province, were used as the research objects, and soil physicochemical factors and soil microbial community characteristics were measured and analyzed. The results showed that the soil water content, soil organic carbon, total nitrogen, and total phosphorus decreased significantly (P<0.05) with increased grassland degradation. In contrast, the trends of soil pH and soil electrical conductivity were opposite. Through high-throughput sequencing, we analyzed and obtained the numbers of bacterial and fungal OTUs in no, light, moderate, and severely degraded grassland were 1927, 2011, 2040, 1798 and 1492, 1511, 1383, 1466; the numbers of bacterial and fungal OTUs specific to no, light, moderate and severely degraded grassland were 588, 513, 691, 409 and 116, 112, 73, 79. The number of soil microbial community bacteria dominated significantly, and the diversity decreased with the degradation of grassland as a whole. The relative abundance of the dominant group of soil bacteria, Proteobacteria, reached a maximum of 45% in the moderately degraded grassland; the relative abundance of the dominant group of soil fungi, Ascomycota, reached a maximum of 89% in the non-degraded grassland. Through RDA analysis, it was obtained that soil electrical conductivity, organic carbon, water content, total nitrogen, total phosphorus and pH were the main influence factors of soil bacteria, and soil total phosphorus, total nitrogen, and organic carbon were the main influence factors of soil fungi.

Key words： Maqu county degraded grassland soil microorganisms high-throughput sequencing technology redundancy analysis

Received: 28 April 2023

PACS:

X53

About author:: X53

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WANG Min
	LIU Min-xia
	WANG Qian-yue
	MIAO Le-le
	YANG Chun-liang
	XIAOYin-di

Cite this article:

WANG Min,LIU Min-xia,WANG Qian-yue等. Soil-microbe characterization and interaction in alpine degraded grassland in Maqu county[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(12): 6482-6489.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I12/6482

[1]	许志信,郭丽珍.加强草原建设促进畜牧业发展[J]. 内蒙古草业, 2000,(3):1-6. Xu Z X, Guo L Z. Strenthenning grassland construction and promoting the development of animalhusbandry[J]. Inner Mongolia Pratacclture, 2000,(3):1-6.
[2]	员旭疆.重新认识草原的地位和作用[J]. 中国畜牧业, 2001,(2):4. Yuan X J. Re-understanding the position and function of grassland[J]. China Animal Industry, 2001,(2):4.
[3]	王庆国,建原,晔薷罕,等.加强草原生态保护和建设实现全区草原资源的可持续发展[J]. 内蒙古草业, 2009,21(4):1-4. Wang Q G, Jian Y, Ye R H, et al. Strengthening grassland ecological protection and construction to realize the sustainable development of grassland resources in the whole region[J]. Inner Mongolia Pratacclture, 2009,21(4):1-4.
[4]	孙小弟,王彦龙.不同退化程度高寒草甸草地生物量及土壤养分差异[J]. 青海畜牧兽医杂志, 2008,38(3):6-8. Sun X D, Wang Y L. Difference of biomass and soil nutrition in alpine-cold meadow of different degraded degree[J]. Chinese Qinghai Journal of Animal and Veterinary Sciences, 2008,38(3):6-8.
[5]	周会程,周恒,肖海龙,等.三江源区不同退化梯度高寒草原土壤重金属含量及其与养分和酶活性的变化特征[J]. 草地学报, 2020,28(3):9. Zhou H C, Zhou H, Xiao H L, et al. The variation characteristics of heavy metal content, nutrient and enzyme activity in soil of alpine steppe with different degradation gradient in the Three River- Headwaters Region[J]. Acta Agrestia Sinica, 2020,28(3):9.
[6]	阿依敏・波拉提,安沙舟,董乙强,等.巴音布鲁克高寒草原不同退化阶段土壤养分的变化[J]. 新疆农业科学, 2017,54(5):953-960. Ayimin Bolati, An S Z, Dong Y Q, et al. Changes of soil nutrients in different degradation stages of alpine steppe in bayanbulak[J]. Xinjiang Agricultural Sciences, 2017,54(5):953-960.
[7]	曾智科.三江源区高寒草甸土壤微生物季节动态及对草地退化的响应[D]. 西宁:青海师范大学, 2009. Zeng Z K. The seasonal dynamics of alpine meadow soil microorganisms and the response from grassland degradation in the area of Three River Source[D]. Xining: Qinghai Normal University, 2009.
[8]	李海云,姚拓,张建贵,等.东祁连山退化高寒草地土壤细菌群落与土壤环境因子间的相互关系[J]. 应用生态学报, 2018,29(11): 3793-3801. Li H Y, Yao T, Zhang J G, et al. Relationship between soil bacterial community and environmental factors in the degraded alpine grassland of eastern Oilian Mountains, China[J]. Chinese Journal of Applied Ecology, 2018,29(11):3793-3810.
[9]	魏强,王芳,陈文业,等.黄河上游玛曲不同退化程度高寒草地土壤物理特性研究[J]. 水土保持通报, 2010,30(5):16-21. Wei Q, Wang F, Chen W Y, et al. Soil physical characteristics on different degraded alpine grasslands in Maqu County in Upper Yellow River[J]. Bulletin of Soil and Water Conservation, 2010,30(5):16-21.
[10]	Coban O, De Deyn G B, van der Ploeg M. Soil microbiota as game-changers in restoration of degraded lands[J]. Science, 2022, 375(6584):abe0725.
[11]	Fierer N, Wood S A, de Mesquita C P B. How microbes can, and cannot, be used to assess soil health[J]. Soil Biology and Biochemistry, 2021,153:108111.
[12]	Harris J. Soil microbial communities and restoration ecology: Facilitators or followers[J]? Science, 2009,325(5940):573-574.
[13]	褚琳.黄河源玛曲高寒湿地生态退化与修复适宜性评价研究[D]. 武汉:华中农业大学, 2012. Chu L. The research on degradation and restoration suitability assessment of Yellow River Source Alpine Wetlands in Maqu[D]. Wuhan: Huazhong Agricultural University, 2012.
[14]	韩海涛,祝小妮.气候变化与人类活动对玛曲地区生态环境的影响[J]. 中国沙漠, 2007,27(4):608-613. Han H T, Zhu X N. Climate change and human activities of Maqu area and lts lmpact on eco-environment[J]. Journal of Desert Research, 2007,27(4):608-613.
[15]	黄国勇,韩茂莉,陈兴鹏.玛曲国家级生态功能保护区分区研究[J]. 地理与地理信息科学, 2003,19(2):5. Huang Y L, Han M L, Chen X P. A study on the regionalization of Maqu national ecological function reserve[J]. Geography and Geo-Information Science, 2003,19(2):5.
[16]	GB 19377-2003天然草地退化、沙化、盐渍化的分级指标[S]. GB 19377-2003 Parameters for degradation, sandification and salification of rangelands[S].
[17]	浦滇,石明,周雪孟,等.基于高通量绝对定量对不同树龄茶树土壤细菌群落多样性的研究[J]. 西南农业学报, 2022,35(1):186-193. Pu D, Shi M, Zhou X M, et al. Soil bacterial community diversity of tea plants with different ages based on high-throughput absolute quantification[J]. Southwest China Journal of Agricultural Sciences, 2022,35(1):186-193.
[18]	蔡丽琼,陈瑞,杨德强,等.基于高通量测序的天麻连作根际土壤真菌群落多样性分析[J]. 中国麻业科学, 2022,44(6):321-330. Cai L Q, Chen R, Yang D Q, et al. Analysis of fungal community diversity in rhizosphere soil of the continuous cropping gastrodia elata based on the high-throughput sequencing[J]. Plant Fiber Sciences in China, 2022,44(6):321-330.
[19]	鲁如坤.土壤农业化学分析方法[M]. 北京:中国农业科技出版社, 2000:106-168. Lu R K. Methods for agrochemical analysis of soils[M]. Beijing: China Agricultural Science and Technology Press, 2000:106-168.
[20]	鲍士旦.土壤农化分析第3版[M]. 北京:中国农业出版社, 2000:22-76. Bao S D. Soil agrochemical analysis (3rd Edition)[M]. Beijing: China Agriculture Press, 2000:22-76.
[21]	吴昊,王理德,宋达成,等.民勤退耕区不同年限退耕地土壤理化性质及酶活性[J]. 干旱地区农业研究, 2021,39(1):191-199. Wu H, Wang L D, Song D C, et al. Soil properties and enzyme activities of abandonedfarmland in different years in Minqin[J]. Agricultural Research in the Arid Areas, 2021,39(1):191-199.
[22]	张瑜斌,邓爱英,庄铁诚,等.潮间带土壤盐度与电导率的关系[J]. 生态环境, 2003,(2):164-165. Zhang Y B, Deng A Y, Zhuang T C. Relation between soil salinity in intertidal zone and electric conductivity[J]. Ecology and Environmental Sciences, 2003,12(2):164-165.
[23]	杨馥铖,刘昌义,胡夏嵩,等.黄河源区不同退化程度高寒草地理化性质及复合体抗剪强度研究[J]. 干旱区研究, 2022,39(2):12. Yang F C, Liu C Y, Hu X S, et al. Study on physical and chemical properties and shear strength characteristics of root-soil composite system with different degradation degrees of alpine grassland in the source region of the Yellow River[J]. Arid Zone Research, 2022,39(2):12.
[24]	姚宝辉,王缠,张倩,等.甘南高寒草甸退化过程中土壤理化性质和微生物数量动态变化[J]. 水土保持学报, 2019,33(3):8. Yao B H, Wang C, Zhang Q, et al. Dynamic characteristics of soil physicochemical properties and microbial quantity during the degradation of Gannan Alpine Meadow[J]. Journal of Soil and Water Conservation, 2019,33(3):8.
[25]	Li H, Qiu Y, Yao T, et al. Nutrients available in the soil regulate the changes of soil microbial community alongside degradation of alpine meadows in the northeast of the Qinghai-Tibet Plateau[J]. Science of The Total Environment, 2021,792:148363.
[26]	李延茂,胡江春,汪思龙,等.森林生态系统中土壤微生物的作用与应用[J]. 应用生态学报, 2004,15(10):4. Li Y M, Hu J C, Wang S L, et al. Function and application of soil microorganisms in forest ecosystem[J]. Chinese Journal of Applied Ecology, 2004,15(10):4.
[27]	王英成,姚世庭,金鑫,等.三江源区高寒退化草甸土壤细菌多样性的对比研究[J]. 生态环境学报, 2022,31(4):695-703. Wang Y C, Yao S T, Jin X, et al. Comparative study on soil bacterial diversity of degraded alpine meadow in the Sanjiangyuan Region[J]. Ecology and Environmental Sciences, 2022,31(4):695-703.
[28]	李海云,姚拓,高亚敏,等.退化高寒草地土壤真菌群落与土壤环境因子间相互关系[J]. 微生物学报, 2019,59(4):11. Li H Y, Yao T, Gao Y M, et al. Relationship between soil fungal community and soil environmental factors indegraded alpine grassland[J]. Acta Microbiologica Sinica, 2019,59(4):11.
[29]	陆梅.纳帕海湿地退化对土壤微生物群落结构及多样性的影响[D]. 北京:北京林业大学, 2018. Lu M. Effects of wetlands degradation on structure and biodiversity of soil microbial community in Napahai plateau wetlands[D]. Beijing: Beijing Forestry University, 2018.
[30]	金志薇,钟文辉,吴少松,等.植被退化对滇西北高寒草地土壤微生物群落的影响[J]. 微生物学报, 2018,58(12):2174-2185. Jin Z W, Zhong W H, Wu S S, et al. Effect of vegetation degradation on microbial communities in alpine grassland soils in Northwest Yunnan[J]. Acta Microbiologica Sinica, 2018,58(12):2174-2185.
[31]	阚海明,庞卓,陈超,等.北京西北浅山区退化草地植被恢复对土壤微生物群落多样性的影响[J]. 草地学报, 2022,30(6):1350-1358. Kan H M, Pang Z, Chen C, et al. Changes in soil microbial communities following the vegetation restoration of degraded sandy grassland in Beijing[J]. Acta Agrestia Sinica, 2022,30(6):1350-1358.
[32]	Steenwerth K L, Jackson L E, Calderón F J, et al. Soil microbial community composition and land use history in cultivated and grassland ecosystems of coastal California[J]. Soil Biology and Biochemistry, 2003,34(11):1599-1611.
[33]	Nie X, Li Z, Huang J, et al. Thermal stability of organic carbon in soil aggregates as affected by soil erosion and deposition[J]. Soil and Tillage Research, 2018,175:82-90.
[34]	Huang J, Li Z, Zeng G, et al. Microbial responses to simulated water erosion in relation to organic carbon dynamics on a hilly cropland in subtropical China[J]. Ecological Engineering, 2013,60:67-75.
[35]	朱怡,吴永波,安玉亭.基于高通量测序的禁牧对土壤微生物群落结构的影响[J]. 生态学报, 2022,42(17):7137-7146. Zhu Y, Wu Y B, An Y T. Effects of grazing prohibition on soil microbial community structure based on high-throughput sequencing[J]. Acta Ecologica Sinica, 2022,42(17):7137-7146.
[36]	谢龙莲,陈秋波,王真辉,等.环境变化对土壤微生物的影响[J]. 热带农业科学, 2004,24(3):39-47. Xie L L, Chen Q B, Wang Z H, et al. Effects of environmental changes on soil microorganisms[J]. Chinese Journal of Tropical Agriculture, 2004,24(3):39-47.
[37]	Bokulich N A, Subramanian S, Faith J J, et al. Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing[J]. Nature Methods, 2013,10(1):57-59.
[38]	Caporaso J G, Kuczynski J, Stombaugh J, et al. QIIME allows analysis of high-throughput community sequencing data[J]. Nature methods, 2010,7(5):335-336.
[39]	姚宝辉,王缠,郭怀亮,等.人工草地建设对甘南草原土壤理化特性和微生物数量特征的影响[J]. 水土保持学报, 2019,33(1):192-199. Yao B H, Wang C, Guo H L, et al. Effects of artificial supplementary sowing on soil physical and chemical characteristics and microorganism quantity in Gannan Grassland[J]. Journal of Soil and Water Conservation, 2019,33(1):192-199.