冬季自然温度下外加AHLs对ANAMMOX生物膜的影响

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Abstract Maintaining the efficient performance of anaerobic ammonia oxidation (ANAMMOX) biofilm under low-temperature conditions is a great challenge. The effects of two exogenous signaling molecules, hexanoyl homoserine lactone (C6-HSL) and octanoyl homoserine lactone (C8-HSL), on the ANAMMOX biofilm process were examined under the natural temperature in winter. The results showed that the addition of C6-HSL and C8-HSL promoted the nitrogen removal performance of the process under low temperature fluctuation, which increased the total nitrogen removal by 48.09% and 44.36%, respectively, and effectively enhanced the adaptability of ANAMMOX biofilm to the low-temperature environment. Under the low-temperature fluctuation, C6-HSL and C8-HSL regulated the activities of hydrazine dehydrogenase (HDH) (1.77 and 1.57 times higher than that of the control group, respectively), which significantly enhanced the activities of ANAMMOX; promoted the secretion of PN and PS in the extracellular polymers (EPS), especially in the TB-EPS, and altered the hydrophobicity of the secondary structure of proteins, which led to enhanced biofilm adhesion performance ( The OD₆₀₀ values were enhanced by 55.12% and 68.32%, respectively). In addition, based on 16S rRNA sequencing, C6-HSL and C8-HSL effectively promoted the growth of Candidatus Kuenenia at low temperature and up-regulated the abundance of functional genes related to nitrogen metabolism.

Key words： ANAMMOX biofilm natural winter temperature quorum sensing

Received: 01 January 2024

PACS:

X703

Corresponding Authors: 李军,教授,lj04778@163.com E-mail: lj04778@163.com

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	LUO Rong
	LI Jun
	GAO Peng
	ZHANG Jing
	XIE Chao-fan
	ZHANG Yi

Cite this article:

LUO Rong,LI Jun,GAO Peng等. Effect of exogenous AHLs on ANAMMOX biofilm process under natural temperature in winter[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(8): 4292-4302.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I8/4292

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