硝酸盐异化还原为铵耦合厌氧氨氧化处理含氮废水

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Abstract To investigate the feasibility and long-term performance of simultaneous removal of ammonium (NH₄⁺) and nitrate (NO₃^-) in wastewater by anaerobic ammonia oxidation (anammox) bacteria, batch, inhibitor, isotopic and long-term reactor experiments were conducted based on the intracellular carbon-dependent dissimilatory nitrate reduction to ammonium (DNRA) of anammox bacteria. In 12h batch experiments, NH₄⁺ and NO₃^- were simultaneously converted to N₂ with anammox biomass concentration of 4500mg/L, and the total nitrogen removal efficiency was 93.49%. In the experiment of acetylene inhibition (inhibiting the process of generating hydrazine from NH₄⁺and nitric oxide in the anammox reaction), 17.01mg N/L of NH₄⁺ was detected, and the contribution rate of anammox bacteria to NO₃^-removal was 85.88%. After the addition of penicillin G (an inhibitor for most heterotrophic denitrifying bacteria), NO₃^- could still be removed. Both ²⁹N₂ and ³⁰N₂ was always detected from the very beginning in the ¹⁵N isotope tracing experiment, and the former was 1.4 folds higher than the latter. In the long-term experiment, the average NH₄⁺ and NO₃^- concentrations of effluent were 1.18 and 2.86mg N/L respectively, reaching an average total nitrogen removal efficiency of 92.26%. These results suggested that NH₄⁺ and NO₃^-could be simultaneously removed by endogenous DNRA coupled with normal anammox metabolism, further promoting the application of anammox-based processes for nitrogen-contained wastewater removal.

Key words： anammox dissimilatory nitrate reduction to ammonium ammonia nitrate

Received: 02 January 2024

PACS:

X703

Corresponding Authors: 乔森,教授,qiaosen@dlut.edu.cn E-mail: qiaosen@dlut.edu.cn

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	ZHAO Shuo
	WANG Chao
	YANG Meng
	QIAO Sen

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ZHAO Shuo,WANG Chao,YANG Meng等. Treatment of nitrogen-containing wastewater by dissimilatory nitrate reduction to ammonium coupled with anaerobic ammonium oxidation[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(8): 4389-4399.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I8/4389

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