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| Thiabendazole-induced cardiac toxicity in early developmental stages of zebrafish |
| GUAN Sheng, HU Bin, ZHU Zhe-ning, WANG Bin-jie, WANG Ji-ye, ZHENG Ruo-nan |
| Key Laboratory of Drug Prevention and Control Technology Research in Zhejiang Province, Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou 310053, China |
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Abstract The development of the heart in zebrafish embryos was examined after exposure to different concentrations of thiabendazole (TBZ) solution (0.06, 0.6, and 6mg/L). The levels of catalase (CAT), superoxide dismutase (SOD), reactive oxygen species (ROS), and the expression of genes related to cardiac development were assessed. The results indicated that exposure to the high concentration of TBZ (6mg/L) caused severe cardiotoxicity, including pericardial edema and a reduced heart rate in zebrafish embryos. This concentration also induced significant oxidative stress in the heart, leading to a large number of apoptotic cells and marked changes in the expression of cardiac development-related genes (gate4, nppa, sox9b, vmhc), as well as apoptosis-related genes (bcl2, bax, puma, p53). These findings suggested that TBZ induced cardiotoxicity by disrupting the normal expression of genes involved in cardiac development, generating oxidative stress, and triggering apoptosis.
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Received: 16 July 2024
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