湿地植物对农村污水耐药基因遗传元件的富集

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Abstract This study aimed to explore the accumulation of MGEs by wetland plants in the treatment of rural sewage using a soil ecological infiltration system. Thus, the changes in the integrase gene intI1and transposase gene tnpA-04 in the vegetative parts of the wetland plant Iris were investigated before and after treatment. The results showed that, over a 60d operational period, the soil ecological infiltration system achieved average removal rates of ammonia nitrogen and chemical oxygen demand from rural sewage of 88.50% and 75.17%, respectively. The average height and fresh weight of the Iris increased by 3.63% and 43.45%, respectively. The concentration of MGEs in the plant increased by 1.67ng/g, with intracellular accounting for 68.26%. Forthermore, the abundance of the tnpA-04 gene was found to be 37.86% higher than that of the intI1gene, which demonstrated a higher propensity for transfer within the vegetative parts of Iris. The bioconcentration ability for MGEs in the plant's vegetative parts followed the order: stem > root > leaf. Moreover, variations in soil properties significantly influenced the plant's ability to accumulate MGEs (P < 0.05). This study suggests that wetland plants can effectively accumulate MGEs from rural sewage, thereby reducing the risk of antibiotic resistance gene dissemination.

Key words： wetland plant soil infiltration sewage horizontal gene transfer mobile genetic elements

Received: 06 September 2024

PACS:	X703
	X173

Corresponding Authors: 黄魁,责任作者,教授,huangk1199@hotmail.com E-mail: huangk1199@hotmail.com

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	WEN Fei-fei
	HUANG Kui
	XIA Hui
	SONG Bing-yu
	ZHAO Meng

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WEN Fei-fei,HUANG Kui,XIA Hui等. Enrichment of antibiotic resistance genetic elements in rural sewage by wetland plants[J]. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(4): 1985-1994.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2025/V45/I4/1985

[1] 张宁,李淼,刘翔.土壤中抗生素抗性基因的分布及迁移转化[J].中国环境科学, 2018,38(7):2609-2617. Zhang N, Li M, Liu X. Distribution and transformation of antibiotic resistance genes in Soil[J]. China Environmental Science, 2018,38(7):2609-2617.
[2] Jang H M, Lee J, Kim Y M, et al. Fate of antibiotic resistance genes and metal resistance genes during thermophilic aerobic digestion of sewage sludge[J]. Bioresource Technology, 2018,249:635-643.
[3] Partridge S R, Kwong S M, Firth N, et al. Mobile genetic elements associated with antimicrobial resistance[J]. Clinical Microbiology Reviews, 2018,31(4):8-17.
[4] 邢月,张雯,程艳,等.西北内陆河抗生素抗性基因赋存特征及其影响因素[J].中国环境科学, 2023,43(6):3077-3086. Xing Y, Zhang W, Cheng Y, et al. Distribution characteristics and influencing factors of antibiotic resistance genes in inland rivers of Northwest China[J]. China Environmental Science, 2023,43(6):3077-3086.
[5] 罗晓,袁立霞,张文丽,等.制药废水厂抗性基因和微生物群落相关性研究[J].中国环境科学, 2019,39(2):831-838. Luo X, Yuan L X, Zhang W L, et al. Correlation study between resistance genes and microbial communities in pharmaceutical wastewater treatment plants[J]. China Environmental Science, 2019, 39(2):831-838.
[6] Haenelt S, Wang G, Kasmanas J C, et al. The fate of sulfonamide resistance genes and anthropogenic pollution marker intI1after discharge of wastewater into a pristine river stream[J]. Front Microbiology, 2023,14:1058350.
[7] Zhang Y X, Zhao Z X, Xu H T, et al. Fate of antibiotic resistance genes and bacteria in a coupled water-processing system with wastewater treatment plants and constructed wetlands in coastal eco-industrial parks[J]. Ecotoxicology and Environmental Safety, 2023, 252:114606.
[8] Zhu Y G, Johnson Timothy A, Su J Q, et al. Diverse and abundant antibiotic resistance genes in Chinese swine farms[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013,110:3435-3440.
[9] Christofilopoulos S, Kaliakatsos A, Triantafyllou K, et al. Evaluation of a constructed wetland for wastewater treatment:addressing emerging organic contaminants and antibiotic resistant bacteria[J]. New Biotechnology, 2019,52:94-103.
[10] Qiao M, Ying G G, Singer A C, et al. Review of antibiotic resistance in China and its environment[J]. Environment International, 2018,110:160-172.
[11] Chen P, Yu K F, He Y L. The dynamics and transmission of antibiotic resistance associated with plant microbiomes[J]. Environment International, 2023,176:107986.
[12] Afzal I, Shinwari Z K, Sikandar S, et al. Plant beneficial endophytic bacteria:Mechanisms, diversity, host range and genetic determinants[J]. Microbiological Research, 2019,221:36-49.
[13] 林小如,李子歆,孙安琪,等.模拟增温对木荷叶际抗生素抗性基因的影响[J].中国环境科学, 2023,43(12):6689-6699. Lin X R, Li Z X, Sun A Q, et al. Effect of stimulated warming on phyllosphere antibiotic resistance genes of Schima superba[J]. China Environmental Science, 2023,43(12):6689-6699.
[14] Liu L M, Han X, Hu J D, et al. Jointly considering multi-medium and full-cycle to better reveal distribution and removal of antibiotic resistance genes in long-term constructed wetland[J]. Science of the Total Environment, 2024,955:177276.
[15] 邵劲博,荣懿,刘星,等.基于污水排放特性的陕南村镇污水处理厂优化运行与节能降耗研究[J].给水排水, 2022,58(8):24-30. Shao J B, Rong Y, Liu X, et al. Study on rural wastewater discharge characteristics and optimal operation of typical township wastewater treatment plants in Southern Shaanxi[J]. Water& Wastewater Engineering, 2022,58(8):24-30.
[16] Mao D Q, Luo Y, Mathieu J, et al. Persistence of extracellular DNA in river sediment facilitates antibiotic resistance gene propagation[J]. Environmental Science& Technology, 2014,48(1):71-78.
[17] Guan M X, Peng L S, Chen J Y, et al. Effects of corncob biochar on the microbial communities and ARGs during vermicomposting of dewatered sludge[J]. China Environmental Science, 2021,41(6):2744-2751.
[18] Cui G Y, Bhat S A, Li W J, et al. Gut digestion of earthworms significantly attenuates cell-free and-associated antibiotic resistance genes in excess activated sludge by affecting bacterial profiles[J]. Science of the Total Environment, 2019,691:644-653.
[19] Huang K, Xia H, Chen J Y, et al. Effects of earthworms on changes of microbial characteristics during vermicomposting of municipal sludge[J]. Acta Scientiae Circumstantiae, 2018,38(8):3146-3152.
[20] HJ 636-2012水质总氮的测定碱性过硫酸钾消解紫外分光光度法[S]. HJ 636-2012 Water quality-Determination of total nitrogen-Alkaline potassium persulfate digestion UV spectrophotometric method[S].
[21] GB 11893-89水质总磷的测定钼酸铵分光光度法[S]. GB 11893-89 Water quality-Determination of total phosphorus-Ammonium molybdate spectrophotometric method[S].
[22] HJ 535-2009水质氨氮的测定纳式试剂分光光度法[S]. HJ 535-2009 Water quality-Determination of ammonia nitrogen-Nessler's reagent spectrophotometry[S].
[23] HJ/T 399-2007水质化学需氧量的测定快速消解分光光度法[S]. HJ/T 399-2007 Water quality-Determination of the chemical oxygen demand-Fast digestion spectrophotometric method[S].
[24] HJ/T 166-2004土壤环境监测技术规范[S]. HJ/T 166-2004 The technical specification for soil environmental monitoring[S].
[25] HJ 1222-2021固体废物水分和干物质含量的测定重量法[S]. HJ 1222-2021 Solid waste-Determination of water content and dry matter-Gravimetric method[S].
[26] HJ 761-2015固体废物有机质的测定灼烧减量法[S]. HJ 761-2015 Solid waste-Determination of organic matter-Ignition loss method[S].
[27] Dan A, Oka M, Fujii Y, et al. Removal of heavy metals from synthetic landfill leachate in lab-scale vertical flow constructed wetlands[J]. Science of the Total Environment, 2017,584:742-750.
[28] Sasmaz A, Obek E, Hasar H. The accumulation of heavy metals in Typha latifolia L. grown in a stream carrying secondary effluent[J]. Ecological Engineering, 2008,33(3/4):278-284.
[29] Martin J W, Asher B J, Beesoon S, et al. PFOS or PreFOS?Are perfluorooctane sulfonate precursors (PreFOS) important determinants of human and environmental perfluorooctane sulfonate (PFOS) exposure?[J]. Journal of Environmental Monitoring, 2010,12(11):1979-2004.
[30] DB 62/4014-2019农村生活污水处理设施水污染物排放标准[S]. DB 62/4014-2019 Discharge standard of pollutants for rural sewage treatment facilities[S].
[31] 张建,黄霞,刘超翔,等.地下渗滤处理村镇生活污水的中试[J].环境科学, 2002,23(6):57-61. Zhang J, Huang X, Liu C X, et al. Pilot study on subsurface wastewater infiltration system applied in rural sewage treatment[J]. Environmental Science, 2002,23(6):57-61.
[32] 秦可娜.土壤渗滤系统深度处理污水厂含抗生素出水的效能与机制[D].哈尔滨:哈尔滨工业大学, 2021. Qin K N. Performance and mechanism of soil aquifer treatment for polishing antibiotics-containing effluent from WWTP[D]. Harbin:Harbin Institute of Technology, 2021.
[33] 黄磊,陈玉成,赵亚琦,等.生物炭添加对湿地植物生长及氧化应激响应的影响[J].环境科学, 2018,39(6):2904-2910. Huang L, Chen Y C, Zhao Y Q, et al. Influence of biochar application on growth and antioxidative responses of macrophytes in subsurface flow constructed wetlands[J]. Environmental Science, 2018,39(6):2904-2910.
[34] Cerqueira F, Matsmoros V, Bayona J M, et al. Antibiotic resistance gene distribution in agricultural fields and crops. A soil-to-food analysis[J]. Environmental Research, 2019,177:108608.
[35] Wang F H, Qiao M, Chen Z, et al. Antibiotic resistance genes in manure[1] amended soil and vegetables at harvest[J]. Journal of Hazardous Materials, 2015,299:215-221.
[36] Dong H, Chen Y L, Wang J, et al. Interactions of microplastics and antibiotic resistance genes and their effects on the aquaculture environments[J]. Journal of Hazardous Materials, 2021,403:123961.
[37] Zhang Y, Li A L, Dai T J, et al. Cell-free DNA:a neglected source for antibiotic resistance genes spreading from WWTPs[J]. Environmental Science& Technology, 2018,52(1):248-257.
[38] Chen P, Guo X Y, Li S N, et al. A review of the bioelectrochemical system as an emerging versatile technology for reduction of antibiotic resistance genes[J]. Environment International, 2021,156:106689.
[39] Li X R, Zhou M, Shi F, et al. Influence of arbuscular mycorrhizal fungi on mercury accumulation in rice (Oryza sativa L.):From enriched isotope tracing perspective[J]. Ecotoxicology and Environmental Safety, 2023,255:114776.
[40] Zhao W, Chen Z B, Yang X Q, et al. Metagenomics reveal arbuscular mycorrhizal fungi altering functional gene expression of rhizosphere microbial community to enhance Iris tectorum's resistance to Cr stress[J], Science of The Total Environment, 2023,895:164970.
[41] Bati C B, Santilli E, Lombardo L. Effect of arbuscular mycorrhizal fungi on growth and onmicronutrient and macronutrient uptake and allocation in olive plantlets growing under high total Mnlevels[J]. Mycorrhiza, 2014,25(2):97-108.
[42] 李港,刘博文,王子晗,等.植物内生菌研究进展[J].现代农业科技, 2022,17:110-113. Li G, Liu B W, Wang Z H, et al. Research progress on plant endophytes[J]. Modern Agricultural Science and Technology, 2022,17:110-113.
[43] Yu T, Liu Z S, Hu B L, et al. Field-based investigation reveals selective enrichment of companion microbes in vegetables leading to specific accumulation of antibiotic resistance genes[J]. Science of the Total Environment, 2024,929:172636.
[44] Serna-Galvis E A, Salazar-Ospina L, Jimx enez J N, et al. Elimination of carbapenem resistant Klebsiella pneumoniae in water by UV-C, UV-C/persulfate and UV-C/H₂O₂. Evaluation of response to antibiotic, residual effect of the processes and removal of resistance gene[J]. Journal of Environmental Chemical Engineering, 2020,8:102196.
[45] Wang L J, Yan X J, Zhu L S, et al. Spread and driving factors of antibiotic resistance genes in soil-plant system in long-term manured greenhouse under lead (Pb) stress[J]. Science of the Total Environment, 2023,855:158756.
[46] Pan Y S, Zong Z Y, Li Y, et al. Complete Sequence of pEC012, a multidrug-resistant IncI1ST71 plasmid carrying blaCTX-M-65, rmtB, fosA3, floR, and oqxAB in an avian Escherichia coli ST117strain[J]. Frontiers in Microbiology, 2016,7:1117.
[47] Yan X X, Jing A, Yin Y C, et al. Heavy metals uptake and translocation of typical wetland plants and their ecological effects on the coastal soil of a contaminated bay in Northeast China[J]. Science of the Total Environment, 2022,803:149871.
[48] Xu H, Chen Z Y, Huang R Y, et al. Antibiotic resistance gene-carrying plasmid spreads into the plant endophytic bacteria using soil bacteria as carriers[J]. Environmental Science& Technology, 2021,55(15):10462-10470.
[49] Chen Q L, Cui H L, Su J Q, et al. Antibiotic resistomes in plant microbiomes[J]. Trends in Plant Science, 2019,24(6):530-541.
[50] DeMalach N. Toward a mechanistic understanding of the effects of nitrogen and phosphorus additions on grassland diversity[J]. Perspectives in Plant Ecology, Evolution and Systematics, 2018,32:65-72.
[51] Liu W X, Jiang L, Yang S, et al. Critical transition of soil bacterial diversity and composition triggered by nitrogen enrichment[J]. Ecology, 2020,101:e03053.
[52] Chai Y N, Schachtman D P. Root exudates impact plant performance under abiotic stress[J]. Trends in Plant Science, 2022,27(1):80-91.
[53] Gao Y J, Luo W, Zhang H F, et al. Enrichment of antibiotic resistance genes in roots is related to specific bacterial hosts and soil properties in two soil-plant systems[J]. Science of the Total Environment, 2023,886:163933.
[54] Li X, Li Y Y, Wu J S. Different in root exudates and rhizosphere microorganisms effect on nitrogen removal between three emergent aquatic plants in surface flow constructed wetlands[J]. Chemosphere, 2023,337:139422.
[55] Hardoim P R, van Overbeek L S, Elsas J D v. Properties of bacterial endophytes and their proposed role in plant growth[J]. Trends in Microbiology 2008,16(10):463-471.
[56] Bulgarelli D, Rott M, Schlaeppi K, et al. Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota[J]. Nature 2012,488(7409):91-95.
[57] 徐双圆,朱家辉,王栋茹,等.植物套作系统修复镉污染农田土壤的效应--以苏南地区为例[J].中国环境科学, 2024,44(6):3289-3300. Xu S Y, Zhu J H, Wang D R, et al. Effect of plant intercropping system on remediation of cadmium contaminated farmland soil-A case study on the southern Jiangsu province area[J]. China Environmental Science, 2024,44(6):3289-3300.
[58] 52Lundberg D S, Lebeis S L, Paredes S H, et al. Defining the core Arabidopsis thaliana root microbiome[J]. Nature, 2012,488:86-90.
[59] 53Bulgarelli D, Rott M, Schlaeppi K, et al. Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota[J]. Nature, 2012,488:91-5.
[60] 54Luan X, Han Z M, Shen Y P, et al. Assessing the effect of treated erythromycin fermentation residue on antibiotic resistome in soybean planting soil:In situ field study[J]. Science of the Total Environment, 2021,779:146329.
[61] 55Liao H, Lu X, Rensing C, et al. Hyperthermophilic composting accelerates the removal of antibiotic resistance genes and mobile genetic elements in sewage sludge[J]. Environmental Science& Technology, 2018,52(1):266-276.
[62] 56Zarraonaindia I, Owens S M, Weisenhorn P, et al. The soil microbiome influences grapevine-associated microbiota[J]. mBio, 2015,6:e02527-14.
[63] 57Chen Q L, An X L, Zhu Y G, et al. Application of struvite alters the antibiotic resistome in soil, rhizosphere, and phyllosphere[J]. Environmental Science& Technology, 2017,51:8149-8157.
[64] 58Liu L, Li J, Xin Y, et al. Evaluation of wetland substrates for veterinary antibiotics pollution control in lab-scale systems[J]. Environmental Pollution, 2021,269:116152.
[65] 59Bengtsson P, Johan, Jonsson, et al. What is the role of the environment in the emergence of novel antibiotic resistance genes?A modeling approach[J]. Environmental Science& Technology, 2021, 55:15734-15743.
[66] 60Chen P, Yu K F, He Y L. The dynamics and transmission of antibiotic resistance associated with plant microbiomes[J]. Environment International, 2023,176:107986.