1. Hunan Academy of Forestry Science, Changsha 410004, China;
2. College of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China;
3. China Eucalypt Research Centre, State Forestry and Grassland Administration, Zhanjiang 524000, China
An ecological restoration engineering project of 4.5hm2 was implemented at a Mn mine tailing site by coplanting two fast-growing trees (Paulownia fortune and Koelreuteriabipinnata) as the dominant colonization species. An organicbacteri-rich manure was used to amend the rhizospheric area surrounding the coplanted seedlings. Enrichment of the site's biodiversity was counted on natural germination of native metal tolerant plant species based on the soil seed inventory capacity. The experimental data showed that the application of the manure not only enhanced the biomass growth and heavy metal uptake of the plant communities but also enriched its species composition and biodiversity. In the sixth year after the establishment of the remediation site, the total dry biomass quantity, total Mn uptake and total number of plant species of the restored plant community with manure treatment reached 33498.7kg/hm2, 5532.6g/hm2 and 51, respectively, which were approximately 22.8, 2.6 and 3.4 times higher than those in the control plot amended with chemical fertilizers. The total uptake of metals (Pb, Cd, Cu, Zn and Cr) by species and the number of species and quantities of rhizospheric microbial communities were also significantly greater in the manure-treated plot. The remediation effect of the organic manure can be attributed to its interaction with plant roots, microorganisms and substrates, forming an integrated system in reduction of the metal toxicity in the rhizosphere. The transplanted Paulownia fortunei and koelreuteriabipinnata grew very fast with characteristics of high levels of biomass quantity, land coverage and metal uptake. The total number of the two coplanted tree species accounted for less than 0.2% of that of the entire plant community in the treatmentplot, however, their dry biomass quantity, Mn uptake, and land coverage contributed to 88%, 73% and 55.2% of the restored ecosystem, respectively. Paulownia fortunei and Koelreuteriabipinnata played important roles as keystone species in the ecological restoration of the manganese tailing site.
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