碳添加对土壤固碳细菌群落结构及多样性的影响——以松嫩平原盐碱耕地为例

苏鑫; 罗慧; 岳中辉; 郭迎岚; 金晓霞; 卢嫚

PDF(450 KB)

中国环境科学 ›› 2020, Vol. 40 ›› Issue (10) : 4496-4503.

土壤污染与控制

碳添加对土壤固碳细菌群落结构及多样性的影响——以松嫩平原盐碱耕地为例

苏鑫, 罗慧, 岳中辉, 郭迎岚, 金晓霞, 卢嫚

作者信息 +

Effects of carbon addition on community structure diversity of CO₂-assimilating bacterial in Saline-alkali cultivated land

SU Xin, LUO Hui, YUE Zhong-hui, GUO Ying-lan, JIN Xiao-xia, LU Man

Author information +

文章历史 +

摘要

采用高通量测序技术，研究秸秆、生物炭和纳米碳3种碳源添加对盐碱耕地土壤固碳细菌群落结构及多样性的影响，并分析土壤化学性质与固碳细菌群落多样性的关系.结果表明：3种碳源添加均降低土壤固碳细菌群落多样性，其中生物炭和纳米碳添加的土壤固碳细菌的Chao1指数、物种多样性、Shannon指数及系统多样性值均高于秸秆添加的.3种碳源添加均降低土壤固碳细菌群落的物种丰度，其中纳米碳添加的物种丰度大于秸秆和生物炭添加的.在群落组成方面及相对丰度上，3种碳源添加后的优势菌门为变形菌门（Proteobacteria），优势菌纲为γ-变形菌纲（Gammaproteobacteria），均在纳米碳添加后相对丰度最高，分别为90.38%、57.79%.群落组间差异分析结果显示，秸秆和纳米碳添加后土壤固碳细菌群落结构差异显著.冗余分析结果表明，土壤固碳细菌群落结构受土壤pH值、有机碳、全氮、全磷、碱解氮及有效磷的综合影响，其中土壤pH值和有效磷含量是影响土壤固碳细菌群落结构的主要化学性质.综合来看，在盐碱耕地中添加秸秆、生物炭或纳米碳，都抑制了土壤固碳细菌群落的多样性和物种丰度，但纳米碳能够增加土壤固碳细菌群落结构差异.

Abstract

The effects of straw(S), biochar(B) and nano-carbon(N) on the structure and diversity of CO₂-assimilating bacteria community in saline-alkali cultivated land were studied with the high-throughput sequencing technology, and the relationship between soil chemical properties and diversity of CO₂-assimilating bacteria community were also analyzed. These three carbon addition sources all reduced the diversity of CO₂-assimilating bacteria community. Chao1index, observed species, Shannon index and PD whole tree of CO₂-assimilating bacteria with B and N addition were higher than those with S addition. These three carbon additions also reduced the species abundance of soil CO₂-assimilating bacteria community. The species abundance of CO₂-assimilating bacteria community in N addition treated samples was higher than that with S and B addition treatments. In terms of community composition and relative abundance, the dominant phylum after these three carbon treatments was Proteobacteria, and the dominant phylum was Gammaproteobacteria. Their relative abundances in N treatment were the highest at 90.38%, and 57.70%, respectively. The results from differences group communities analysis showed that there were significant differences in the community structure of CO₂-assimilating bacteria after the addition of S and N. The results from redundancy analysis showed that the structure of CO₂-assimilating bacteria community was influenced by soil pH, organic carbon, total nitrogen, total phosphorus, available nitrogen and phosphorus. Soil pH and active phosphorus content were the main soil chemical properties affecting the structure of soil CO₂-assimilating bacteria community. Our findings suggested that the addition of S, B or N in saline-alkali cultivated land could inhibit the diversity and species abundance of soil CO₂-assimilating bacteria community, but the addition of N could increase the structure difference of soil CO₂-assimilating bacteria community.

导出引用

苏鑫, 罗慧, 岳中辉, 郭迎岚, 金晓霞, 卢嫚. 碳添加对土壤固碳细菌群落结构及多样性的影响——以松嫩平原盐碱耕地为例[J]. 中国环境科学. 2020, 40(10): 4496-4503

SU Xin, LUO Hui, YUE Zhong-hui, GUO Ying-lan, JIN Xiao-xia, LU Man. Effects of carbon addition on community structure diversity of CO₂-assimilating bacterial in Saline-alkali cultivated land[J]. China Environmental Science. 2020, 40(10): 4496-4503

中图分类号： X53

参考文献

[1] 王志春,李取生,李秀军,等.松嫩平原盐碱化土地治理与农业持续发展对策[J]. 中国生态农业学报, 2004,12(2):166-168. Wang Z H, Li Q S, Li X J, et al. Saline-alkali land management and countermeasures of sustainable agricultural development in Songnen Plain[J]. Chinese Journal of Eco-Agriculture, 2004,12(2):166-168.
[2] 王遵亲.中国盐渍土[M]. 北京:科学出版社, 1993:250-253. Wang Z Q. Saline soil in China[M]. Beijing:Science Press, 1993:250-253.
[3] Wang J, Wang X, Xu M, et al. Crop yield and soil organic matter after long-term straw return to soil in China[J]. Nutrient Cycling in Agroecosystems, 2015,102(3):371-381.
[4] Loke P F, Kotzé E, Du Preez C C. Changes in soil organic matter indices following 32years of different wheat production management practices in semi-arid South Africa[J]. Nutrient Cycling and Agroecosystems, 2012,94(1):97-109.
[5] 田慎重,宁堂原,王瑜,等.不同耕作方式和秸秆还田对麦田土壤有机碳含量的影响[J]. 应用生态学报, 2010,21(2):373-378. Tian S Z, Ning T Y, Wang Y, et al. Effects of different tillage methods and straw-returning on soil organic carbon content in a winter wheat field[J]. Journal of Applied Ecology, 2010,21(2):373-378.
[6] 周雅心,王晓彤,王广磊,等.炉渣与生物炭施加对稻田土壤细菌多样性及群落组成的影响[J]. 中国环境科学, 2020,40(3):1213-1223. Zhou Y X, Wang X T, Wang G L, et al. Effect of the slag and biochar application on bacterial diversity and community composition of paddy field[J]. China Environmental Science, 2020,40(3):1213-1223.
[7] 牛怡,张仁陟,蔡立群,等.促腐条件下小麦玉米秸秆还田土壤养分变化特征研究[J]. 干旱地区农业研究, 2015,33(2):152-158. Niu Y, Zhang R S, Cai L Q, et al. Dynamic characteristics of fertilized soil with wheat and corn straws under decomposition conditions[J]. Agricultural Research in The Arid Areas, 2015,33(2):152-158.
[8] 秦川,何丙辉,蒋先军.三峡库区不同土地利用方式下土壤养分含量特征研究[J]. 草业学报, 2016,25(9):10-19. Qin C, He B H, Jiang X J. Soil nutrient characteristics of different land-use types in the Three Gorges Reservoir[J]. Acta Prataculturae Sinica, 2016,25(9):10-19.
[9] 宋大利,侯胜鹏,王秀斌,等.中国秸秆养分资源数量及替代化肥潜力[J]. 植物营养与肥料学报, 2018,24(1):1-21. Song D L, Hou H P, Wang X B, et al. Nutrient resource quantity of crop straw and its potential of substituting[J]. Journal of Plant Nutrition and Fertilizers, 2018,24(1):1-21.
[10] Frey S D, Six J, Elliott E T. Reciprocal transfer of carbon and nitrogen by decomposer fungi at the soil-litter interface[J]. Soil Biology and Biochemistry, 2003,35(7):1001-1004.
[11] Ding X, Zhang X, He H. Dynamics of soil amino sugar pools during decomposition processes of corn residues as affected by inorganic N addition[J]. Journal of Soils and Sediments, 2010,10(4):758-766.
[12] 顾美英,唐光木,刘洪亮,等.施用棉秆炭对新疆连作棉花根际土壤微生物群落结构和功能的影响[J]. 应用生态学报, 2016,27(1):173-181. Gu M Y, Tang G M, Liu H L, et al. Effects of cotton stalk biochar on microbial community structure and function of continuous cropping cotton rhizosphere soil in Xinjiang, China[J]. Journal of Applied Ecology, 2016,27(1):173-181.
[13] 贾相岳.玉米秸秆造夹层处理对盐碱地土壤微生物数量的影响[J]. 南方农业, 2017,11(10):24-27. Jia X Y. Effects of corn stover interlay treatment on microorganisms in saline-alkali Soil[J]. South China Agriculture, 2017,11(10):24-27.
[14] 周玉祥,宋子岭,孔涛,等.不同秸秆生物炭对露天煤矿排土场土壤微生物数量和酶活性的影响[J]. 环境化学, 2017,36(1):106-113. Zhou Y X, Song Z L, Kong T, et al. Effect of straw biochar on soil microbe number and soil enzyme activities in opencast coal mine dump[J]. Environmental Chemistry, 2017,36(1):106-113.
[15] 何瑞成,吴景贵.有机物料对原生盐碱地土壤生物学性质的影响[J]. 土壤学报, 2018,55(3):774-782. He R C, Wu J G. Effect of amendment of organic materials on soil biological property in primary saline alkali soil[J]. Acta Pedologica Sinica, 2018,55(3):774-782.
[16] 侯建伟,邢存芳,卢志宏,等.不同秸秆生物炭对贵州黄壤细菌群落的影响[J]. 中国农业科学, 2018,51(23):4485-4495. Hou J W, Xing C F, Lu Z H, et al. Effects of the different crop straw biochars on soil bacterial community of yellow soil in Guizhou[J]. Scientia Agricultura Sinica, 2018,51(23):4485-4495.
[17] Karhu K, Mattilab T, Brgstroma I, et al. Biochar addition to agricultural soil increased CH₄uptake and water holding capacity-results from a short-term pilot field study[J]. Agriculture,Ecosystem and Environment, 2011,1(40):309-313.
[18] 高荣广,赵鑫,高晓兰,等.纳米碳对桃园土壤肥力及植株养分吸收的影响[J]. 落叶果树, 2018,50(3):11-14. Gao R G, Zhao X, Gao X L, et al. Effects of nano carbon on soil fertility and plant nutrient uptake in peach orchard[J]. Deciduous Fruits, 2018,50(3):11-14.
[19] 史妍君,钟建,黄占斌,等.纳米碳增效剂对柑橘产量及土壤细菌种群结构的影响[J]. 中国土壤与肥料, 2018,(4):40-47. Shi Y J, Zhong J, Huang Z B, et al. Effects of super absorbent polymer of nano carbon on citrus yield and soil bacterial communities structure[J]. Soil and Fertilizer Sciences in China, 2018,(4):40-47.
[20] 苏鑫,郭迎岚,冯程程,等.3种碳添加对退化农田土壤固碳细菌群落结构多样性的影响[J]. 环境科学学报, 2020,40(1):234-241. Su X, Guo Y L, Feng C C, et al. Effects of three kinds of carbon addition on community structure diversity of CO₂-assimilating bacterial in degraded farmland soil[J]. Acta Scientiae Circumstantiae, 2020,40(1):234-241.
[21] 鲍士旦.土壤农化分析[M]. 北京:中国农业科技出版社, 2000:24-76. Bao S D. Agrochemical analysis of soil[M]. Beijing:China Agricultural Science and Technology Press, 2000:24-76.
[22] 季凤云,明红霞,李洪波,等.南海表层海水参与卡尔文循环的固碳基因多样性研究[J]. 环境科学学报, 2016,36(11):4037-4043. Ji F Y, Ming H X, Li H B, et al. Diversity of CO₂ fixation gene in the surface waters of northern South China Sea in the Calvin cycle[J]. Acta Scientiae Circumstantiae, 2016,36(11):4037-4043.
[23] Edgar R C. UPARSE:Highly accurate OTU sequences from microbial amplicon reads[J]. Nature Methods, 2013,10(10):996-1000.
[24] Yuan H, Ge T, Wu X, et al. Long-term field fertilization alters the diversity of autotrophic bacteria based on the ribuose, 5-biphosphate carboxylase/oxygenase (RubisCO) large-subunit genes in paddy soil[J]. Applied Microbiology and Biotechnology, 2012,95(4):1061-1071.
[25] Jiang X, Peng X, Deng G, et al. Illumina sequencing of 16S rRNA tag revealed spatial variations of bacterial communities in a mangrove wetland[J]. Microbiology Ecology, 2013,66(1):96-104.
[26] Yuan M, Ge T, Zou S, et al. Effect of land use on the abundance and diversity of autotrophic bacteria as measured by ribulose, 5-biphosphate carboxylase/oxygenase (Rubis CO) large subumit gene abundance in soils[J]. Biology and Fertility of Soils, 2013,49(5):609-616.
[27] Marris E. Putting the carbon back:Black is the new green[J]. Nature, 2006,442(3):624-626.
[28] 武爱莲,丁玉川,焦晓燕,等.玉米秸秆生物炭对褐土微生物功能多样性及细菌群落的影响[J]. 中国生态农业学报, 2016,24(6):736-743. Wu A L, Ding Y C, Jiao X Y, et al. Effect of corn-stalk biochar on soil microbial functional diversity and bacterial community in cinnamon soils[J]. Chinese Journal of Eco-Agriculture, 2016,24(6):736-743.
[29] Jin H. Characterization of microbial life colonizing biochar and biochar-amended soils[D]. New York:Cornell University, Ithaca, 2010.
[30] 刘琼,魏晓梦,吴小红,等.稻田土壤固碳功能微生物群落结构和数量特征[J]. 环境科学, 2017,38(2):760-768. Liu Q, Wei X M, Wu X H, et al. Characteristic of abundances and diversity of carbon dioxide fixation microbes in paddy soils[J]. Environmental Science, 2017,38(2):760-768.
[31] 戴雅婷,闫志坚,解继红,等.基于高通量测序的两种植被恢复类型根际土壤细菌多样性研究[J]. 土壤学报, 2017,54(3):35-748. Dai Y T, Yan Z J, Jie J H, et al. Soil bacteria diversity in rhizosphere under two types of vegetation restoration based on high throughput sequencing[J]. Acta Pedologica Sinica, 2017,54(3):35-748.
[32] 李凤霞,郭永忠,许兴.盐碱地土壤微生物生态特征研究进展[J]. 安徽农业科学, 2011,39(23):14065-14067,14174. Li F X, Guo Y Z, Xu X. Research progress of the microbial characteristics of saline-alkali soil[J]. Journal of Anhui Agricultural Sciences, 2011,39(23):14065-14067,14174.
[33] 薛文强,周蓓蓓,毛通,等.纳米碳混合层对土壤水分入渗特性及水分分布影响[J]. 水土保持学报, 2018,32(3):152-159. Xue W Q, Zhou B B, Mao T, et al. Influence of nano-carbon composite layer on soil water infiltration and water distribution[J]. Journal of Soil and Water Conservation, 2018,32(3):152-159.
[34] Mizanur. Nutrient-use and carbon-sequestration efficiencies in soils from different organic wastes in rice and tomato cultivation[J]. Communications in Soil Science & Plant Analysis, 2013,44(9):1457-1471.
[35] Lehmann J, Rillig M C, Thies J, et al. Biochar effects on soil biota-A review[J]. Soil Biology and Biochemistry, 2011,43(9):1812-1836.
[36] Ameloot N, Graber E R, Verheijen F G A, et al. Interactions between biochar stability and soil organisms:review and research needs[J]. European Journal of Soil Science, 2013,64(4):379-390.
[37] Masiello C A, Chen Y, Gao X D, et al. Biochar and microbial signaling:production conditions determine effects on microbial communication[J]. Environmental Science & Technology, 2013,47(20):11496-11503.
[38] Nele V, Fabian K, Ken D S, et al. Soil quality as affected by tillage residue management in a wheat-maize irrigated bed planting system[J]. Plant and Soil, 2011,340(1):453-466.