Abstract：In order to explore the effects of dissolved organic matter (DOM) composition and source on bacterial community structure and diversity, and the role of bacterial community structure in controlling DOM composition and degradation, the fluorescence components and spatial distribution of DOM in Bahe River during dry season (December) were examined using three-dimensional fluorescence spectroscopy combined with parallel factor analysis (EEM-PARAFAC). The results showed that the DOM fluorescent components of Bahe River were divided into five components by PARAFAC, including three humus components (C1:240,320/400nm; C2:260~295/478~504nm; C5:240/480nm) and two protein components (C3:240,272~284/350~360nm; C4:255~275/326~336nm). A relatively strong correlation was observed among the five components, respectively. It was shown that these two types of fluorescent components had similar source properties. Proteobacteria (54.22%), Bacteroidetes (19.40%), Firmicutes (12.28%), Cyanobacteria (6.46%) and Actinobacteria (3.13%) were the dominant phylum in Bahe River. The nutrient concentration, water temperature, electrical conductivity and pH of river water had effects on the bacterial community. The correlation between fluorescent components (humic-like and proteinic-like) of DOM and the bacterioplankton community was significant along the longitudinal gradient of the river. Different bacterial communities may have different responses to the quality and instability of DOM, which can provide information on the effects of biogeochemical processes of rivers on the composition and function of the microbial population.
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