A recovery reregulation was adopted for a completely autotrophic nitrogen removal over nitrite (CANON) system, for which the shortcut nitrification process deteriorated. The evolution of the microbial construct was evaluated by 16S rDNA sequencing technology during the recovery regulation period, paying special attention to nitrifiers and anammox bacteria. The high concentration of NH4+-N (over 300mg/L) and extending the ratio of aeration/non-aeration to 1:3could effectively inhibit nitrite-oxidizing bacteria (NOB). In the later period of the stable phases, the nitrite-oxidizing rate (NOR) and ΔNO3--N/ΔNH4+-N decreased to 2.30g N/(m3·h) and 0.13respectively; while, the total nitrogen removal rate (TNRR) gradually increased to 0.35kg N/(m3·d). Then, extending the non-aeration time to 90min reduced NOR to nearly zero. The results of 16S rDNA gene high-throughput sequencing indicated that the relative abundance of Nitrospira, the major bacteria of NOB, decreased from 3.96% to 0.64%, and the relative abundance of Candidatus_Jettenia, the major bacteria of anaerobic ammonia-oxidizing bacteria (AnAOB), increased to 49.98% from 46.68%. Our findings elucidated that increasing the influent concentration of ammonia nitrogen can inhibit and eliminate NOB effectively, making AnAOB get enrichment and favoring CANON system recovery.
张铃敏, 常青龙, 史勤, 赵宇豪, 谢弘超, 王亚宜. CANON工艺短程硝化恢复调控及微生物种群结构变化[J]. 中国环境科学, 2019, 39(6): 2354-2360.
ZHANG Ling-min, CHANG Qing-long, SHI Qin, ZHAO Yu-hao, XIE Hong-chao, WANG Ya-yi. The recovery regulation of a CANON system and variations in the microbial community. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(6): 2354-2360.
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