黄土区侵蚀坡面土壤微生物群落功能多样性研究

朱世硕; 夏彬; 郝旺林; 许明祥

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中国环境科学 ›› 2020, Vol. 40 ›› Issue (9) : 4099-4105.

环境微生物

黄土区侵蚀坡面土壤微生物群落功能多样性研究

朱世硕¹, 夏彬^2,3, 郝旺林^2,3, 许明祥^1,2,3

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Functional diversity of soil microbial community on eroded slope in the Loess Plateau Region

ZHU Shi-shuo¹, XIA Bin^2,3, HAO Wang-lin^2,3, XU Ming-xiang^1,2,3

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摘要

为研究不同有机碳水平下坡面土壤侵蚀对微生物群落功能多样性的影响，以黄土区典型侵蚀坡面为对象，通过Biolog-Eco方法测定了高、低2个有机碳水平坡面沉积区、侵蚀区和对照区3个不同部位的土壤微生物活性、碳源利用能力和微生物群落功能多样性.结果表明：1）高碳水平下3个部位的微生物活性和碳源利用能力均显著高于低碳水平；2）在不同侵蚀部位上，沉积区微生物活性高于侵蚀区，在低碳水平下，沉积区微生物碳源利用能力显著大于侵蚀区（P<0.05），但在高碳水平下沉积区与侵蚀区无显著差异；3）在6类碳源中，微生物对氨基酸类、酯类的利用率最高，对醇类、胺类的利用率较低，从31种碳源中提取的主成分1和主成分2分别可解释变量方差的53.8%和11.9%，对主成分1起分异作用的主要是氨基酸类和酯类碳源；对主成分2起分异作用的主要为糖类和酸类碳源.4）高碳水平下3个部位微生物群落的Shannon指数、丰富度指数和McIntosh指数均显著大于低碳水平（P<0.05），但不同部位间的土壤微生物群落多样性指数并无明显差异.侵蚀对微生物群落功能多样性的影响主要表现在有机碳水平决定的微生物活性差别上，不同部位微生物的碳源利用能力在有机碳水平较低时差异显著，有机碳水平较高时则没有明显差别.

Abstract

Soil microbial activity and community functional diversity at the sedimentary area, erosion area and control area on the slopes with two organic carbon levels were determined by the Biolog-Eco method in the Loess Plateau region so as to explore the effects of erosion and organic carbon levels on the functional diversity of microbial communities. The results showed: 1) The microbial activity and carbon-use ability in the three areas of the slopes under the high carbon level were significantly higher than that under the low carbon level. 2) The microbial activity in the sedimentary area was higher than erosion area under the same carbon levels. However, the carbon-use capacity of microbes in the sedimentary aera was significantly higher than that in the erosion area (P<0.05)at low carbon level. No significant difference was found in the carbon-use capacity of microbes between sedimentary and erosion areas at high carbon level. 3) The microbes had the highest utilization on amino acids and esters but lowest utilization on alcohol and amines among the six types of carbon sources. The principal components 1and 2 extracted from 31 carbon sources could explain 53.8% and 11.9% of variance variables, respectively. Amino acids and ester carbon sources were the main carbon sources that differentiate the PC1, and carbohydrates and acids were the main carbon sources that differentiate the PC2. 4) The Shannon index, richness index and McIntosh index of microbial communities under high carbon levels were significantly greater than that under low carbon levels at the same area on the slopes (P<0.05), However, the diversity of microbial communities in different areas of the slopes was no obvious difference under the same carbon level. The impact of erosion on the functional diversity of microbial communities was mainly caused by the difference of microbial activity, which related to the organic carbon level. The carbon-use ability of microbes in different areas was significantly different under a low organic carbon level, but no obvious difference was found under the higher organic carbon level.

导出引用

朱世硕, 夏彬, 郝旺林, 许明祥. 黄土区侵蚀坡面土壤微生物群落功能多样性研究[J]. 中国环境科学. 2020, 40(9): 4099-4105

ZHU Shi-shuo, XIA Bin, HAO Wang-lin, XU Ming-xiang. Functional diversity of soil microbial community on eroded slope in the Loess Plateau Region[J]. China Environmental Science. 2020, 40(9): 4099-4105

中图分类号： X144

参考文献

[1] Cerdan O, Govers G, Le Bissonnais Y, et al. Rates and spatial variations of soil erosion in Europe:A study based on erosion plot data[J]. Geomorphology, 2010,122(1):167-177.
[2] Lal R. Soil carbon sequestration impacts on global climate change and food security[J]. Science, 2004,304:1623-1627.
[3] Van Oost K, Quine T A, Govers G, et al. The impact of agricultural soil erosion on the global carbon cycle[J]. Science, 2007,318(5850):626-629.
[4] 杨钙仁,童成立,张文菊,等.陆地碳循环中的微生物分解作用及其影响因[J]. 土壤通报, 2005,36(4):605-609. Yang G R, Tong C L, Zhang W J, et al. Decom position of organic matter by soil microorganisms in terrestrial carbon cycling and its influence factors[J]. Chinese Journal of Soil Science, 2005,36(4):605-609.
[5] 王娜,高婕,魏静,等.三江平原湿地开垦对土壤微生物群落结构的影响[J]. 环境科学, 2019,40(5):2375-2381. Wang N, Gao J, Wei J, et al. Effects of wetland reclamation on soil microbial community structure in thesanjiang plain[J]. Environmental Science, 2019,40(5):2375-2381.
[6] 王超华,许明祥.黄土丘陵区不同有机碳水平侵蚀坡面土壤微生物量碳的分布特征[J]. 环境科学学报, 2015,35(10):3284-3291. Wang C H, Xu M X, Ran Y F, et al. Distribution of soil microbial biomass on eroded sloping land with different organic carbon contents in the hilly loess plateau region[J]. Acta Scientiae Circumstantiae, 2015,35(10):3284-3291.
[7] 覃乾,朱世硕,夏彬,等.黄土丘陵区侵蚀坡面土壤微生物量碳时空动态及影响因素[J]. 环境科学, 2019,40(4):1973-1980. Qin Q, Zhu S S, Xia B, et al. Temporal and spatial dynamics of soil microbial biomass carbon and its influencing factors on an eroded slope in Hilly Loess Plateau Region[J]. Environmental Science, 2019,40(4):1973-1980.
[8] 喻为,李忠武,黄金权.水力侵蚀影响下土壤有机碳和微生物数量动态变化特征[J]. 土壤学报, 2015,52(2):423-430. Yu W, Li Z W, Huang J Q, et al. Dynamics of soil microbial population and organic carbon under water erosion[J]. Acta Pedologica Sinica, 2015,52(2):423-430.
[9] Huang J Q, Li Z W, Zeng G M, et al. Microbial responses to simulated water erosion in relation to organic carbon dynamics on a hilly cropland in subtropical China[J]. Ecological Engineering, 2013, 60(40):67-75.
[10] Dungait J A, Ghee C, Rowan J S, et al. Microbial responses to the erosional redistribution of soil organic carbon in arable fields[J]. Soil Biology and Biochemistry, 2013,60:195-201.
[11] Delgado-Baquerizo M, Giaramida L, Reich P B, et al. Lack of functional redundancy in the relationship between microbial diversity and ecosystem functioning[J]. Journal of Ecology, 2016,104(4):936-946.
[12] Abdullah M, Al-Dhabaan F, Hassan Bakhali A. Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community[J]. Saudi Journal of Biological Sciences, 2017, 24(4):901-906.
[13] Ma W M, Li Z W, Ding K Y, et al. Effect of soil erosion on dissolved organic carbon redistribution in subtropical red soil under rainfall simulation[J]. Geomorphology, 2014,226:217-225.
[14] Li Z W, Xiao H B, Tang Z H, et al. Microbial responses to erosion-induced soil physico-chemical property changes in the hilly red soil region of southern China[J]. European Journal of Soil Biology, 2015,71:37-44.
[15] 安丽芸,李君剑,严俊霞,等.微生物多样性对土壤碳代谢特征的影响[J]. 环境科学, 2017,38(10):4420-4426. An L Y, Li J J, Yan J X, et al. Effects of microbial diversity on soil carbon mineralization[J]. Environmental Science, 2017,38(10):4420-4426.
[16] Nie X D, Li Z W, Huang J Q, et al. Thermal stability of organic carbon in soil aggregates as affected by soil erosion and deposition[J]. Soil & Tillage Research, 2018,175:82-90.
[17] 张宇婷,肖海兵,聂小东,等.基于文献计量分析的近30年国内外土壤侵蚀研究进展[J]. 土壤学报, 2019,10:1-19. Zhang Y T, Xiao H B, Nie X D, et al. Evolution of research on soil erosion at home and abroad in the past 30years——Based on bibliometric analysis[J]. Acta Pedologica Sinica, 2019,10:1-19.
[18] 田雅楠,王红旗.Biolog法在环境微生物功能多样性研究中的应用[J]. 环境科学与技术, 2011,34(3):50-57. Tian Y N, Wang H Q. Application of biolog to study of environmental microbial function diversity[J]. Environmental Science &Technology, 2011,34(3):50-57.
[19] Räsänen N, Kankaala P, Tahvanainen T, et al. Changes in dissolved organic matter and microbial activity in runoff waters of boreal mires after restoration[J]. Aquatic Sciences, 2018,80:20-34.
[20] Sergio A, Luisella C, Ruby J, et al. Effect of long-term soil management on the mutual interaction among soil organic matter, microbial activity and aggregate stability in a vineyard[J]. Pedosphere, 2018,28(2):288-298.
[21] 张恩平,田悦悦,李猛,等.长期不同施肥对番茄根际土壤微生物功能多样性的影响[J]. 生态学报, 2018,38(14):5027-5036. Zhang E P, Tian Y Y, Li M, et al. Effects of various long-term fertilization regimes on soil microbial functional diversity in tomato rhizosphere soil[J]. Acta Ecologica Sinica, 2018,38(14):5027-5036.
[22] Ke H, Li H X, Lu W S, et al. Effect of microbial organic fertilizer application on soil microbial activity[J]. Chinese Journal of Eco-agriculture, 2010,18(2):303-306.
[23] Xiao H B, Li Z W, Dong Y T, et al. Changes in microbial communities and respiration following the revegetation of eroded soil[J]. Agriculture Ecosystems & Environment, 2017,246:30-37.
[24] 王强,戴九兰,吴大千,等.微生物生态研究中基于BIOLOG方法的数据分析[J]. 生态学报, 2010,30(3):817-823. Wang Q, Dai J L, Wu D Q, et al. Statistic alanalysis of data from BIOLOG method in the study of microbial ecology[J]. Acta Ecologica Sinica, 2010,30(3):817-823.
[25] Ge Z W, Du H J, Gao Y L, et al. Analysis on metabolic functions of stored rice microbial communities by BIOLOG-ECO Microplates[J]. Frontiers in microbiology, 2018,9:1375-1383.
[26] 王楠楠,韩冬雪,孙雪,等.降水变化对红松阔叶林土壤微生物功能多样性的影响[J]. 生态学报, 2017,37(3):868-876. Wang N N, Han D X, Sun X, et al. Effects of precipitation change on soil microbial functional diversity in the primitive Korean pine and broadleaved forests[J]. Acta Ecologica Sinica, 2017,37(3):868-876.
[27] 张超,刘国彬,薛萐,等.黄土丘陵区不同植被类型根际微生物群落功能多样性研究[J]. 草地学报, 2015,23(4):710-717. Zhang C, Liu G B, Xue S, et al. Functional diversity of rhizosphere microbial community of different vegetation types in the hilly-gully region of Loess Plateau[J]. Acta Agrestia Sinica, 2015,23(4):710-717.
[28] Wang Y, Li F C, Zhao J, et al. Microbial community diversity and vertical distribution in a columnar sediment of Maluku Strait[J]. Journal of Atmospheric Science Research, 2019,2:51-58.
[29] 吴则焰,林文雄,陈志芳,等.武夷山国家自然保护区不同植被类型土壤微生物群落特征[J]. 应用生态学报, 2013,24(8):2301-2309. Wu Z Y, Lin W X, Chen Z F, et al. Characteristics of soil microbial community under different vegetation types in Wuyishan National Nature Reserve, East China[J]. Chinese Journal of Applied Ecology, 2013,24(8):2301-2309.
[30] 李飞,刘振恒,贾甜华,等.高寒湿地和草甸退化及恢复对土壤微生物碳代谢功能多样性的影响[J]. 生态学报, 2018,38(17):6006-6015. Li F, Liu Z H, Jia T H, et al. Functional diversity of soil microbial community carbon metabolism with the degradation and restoration of alpine wetlands and meadows[J]. Acta Ecologica Sinica, 2018,38(17):6006-6015.
[31] Piotrowska-Długosz A, Breza-Boruta B, Długosz J. Spatio-temporal heterogeneity of soil microbial properties in a conventionally managed arable field[J]. Journal of Soils & Sediments, 2019,19(1):345-355.
[32] 刘怡萱,曹鹏熙,马红梅,等.青藏高原土壤微生物多样性及其影响因素研究进展[J]. 环境生态学, 2019,1(6):1-7. Liu Y X, Cao P X, Ma H M, et al. Research progress on soil microbial diversity and its influencing factors in Qinghai-Tibet Plateau[J]. Environmental Ecology, 2019,1(6):1-7.