Effect of biochar on metabolic activity of soil microbial carbon
ZHOU Feng, XU Chen-yang, JIN Yong-liang, FU Zi-teng, YAN Heng-nian, CHEN Wen-yi, CHEN Si-mo, GENG Zeng-chao
Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture, College of Natural Resources and Environment, Northwest A & F University, Yangling 712100, China
Field experiment was conducted to investigate the effect of biochar application on the functional diversity of microbial community in rhizosphere and bulk soils. The average well color development (AWCD) during microbial carbon metabolism, Richness index, Shannon-Wiener index, Evenness index, Simpson index of microorganisms in rhizosphere and bulk soils were analyzed by the biolog-ECO method under application of different amounts of biochar. Results showed that the metabolic activity of microbial carbon in rhizosphere soil was increased due to biochar application. When the biochar addition was equal or above 40t/hm2, the metabolic activity of microbial carbon was increased significantly. Biochar application had no significant effect on the metabolic activity of microbial carbon in bulk soil. Meanwhile, the Richness index, Shannon-Wiener index, Evenness index of microbial communities in both rhizosphere and bulk soils were not significantly affected. Biochar application enhanced the ability of soil organisms in rhizosphere to utilize carbohydrates, amino acids and carboxylic acids as carbon sources, while it did not significantly influence the carbon utilization ability of soil organisms in bulk soil. Organic carbon was the main factor that controlled the metabolic activity of microbial carbon. Biochar addition of 40t/hm2 was the most suitable application amount.
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