Damage to human corneal epithelial cells caused by indoor dust in plateau city
BI Jue1,2, ZHANG Zhen-ning2, LIU Yu-jia2, YANG Dan-lei2, ZHENG Qin-xiang3, CUI Dao-lei2, XIANG Ping2
1. National Plateau Wetland Research Center, College of Wetland, Southwest Forestry University, Kunming 650224, China; 2. Institute of Environment Remediation and Human Health, School of Ecology and Environment, Southwest Forestry University, Kunming 650224, China; 3. Eye Hospital of Wenzhou Medical University, Wenzhou 325027, China
Abstract:The indoor dust samples were collected from houses, office, and dormitory in a typical high-altitude city Kunming. Each sample was separated into 4size fractions (F1~F4: 100~2000, 75~100, 63~75, 45~63μm), and the concentrations of nine heavy metals (Pb, Cu, Cd, Zn, Cr, Ni, Co, As, and Mn) in indoor dust were detected using ICP-OES. In addition, the solubility rates (bioaccessibility) of the target heavy metals in artificial tears extraction and their cytotoxicity on human corneal epithelial cells (HCECs) were determined. The results showed that Ni, Mn, and Zn in office dust were significantly higher than those in residence and dormitory dust (P<0.01), and the contents of Cd, Ni, and Zn were 39.3, 9.5 and 12.3times higher than the background values of Kunming soils. The solubility rates of Co in residence dust, As in office dust and Mn in dormitory dust were the highest at 10.6%, 16.1%, and 36%, respectively. Heavy metals were highly susceptible to accumulate in office and dormitory dust with particle size ranging from 63 to 100μm. Moreover, the typical polygonal and cobblestone appearance of HCECs became spindle shape after artificial tear extracts of office dust exposure. Cell proliferation was enhanced at 20μg/mL whereas inhibited at higher concentration (P<0.05). The elevated mRNA expression of pro-inflammatory mediators (IL-1β and TNF-α) suggested that indoor dust from Kunming may pose a risk to inducing human corneal inflammation.
毕珏, 张振宁, 刘雨佳, 杨丹蕾, 郑钦象, 崔道雷, 向萍. 高原城市室内灰尘对人角膜上皮细胞损伤研究[J]. 中国环境科学, 2021, 41(7): 3360-3370.
BI Jue, ZHANG Zhen-ning, LIU Yu-jia, YANG Dan-lei, ZHENG Qin-xiang, CUI Dao-lei, XIANG Ping. Damage to human corneal epithelial cells caused by indoor dust in plateau city. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(7): 3360-3370.
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