采用超声波-UV-LED联合工艺以期实现对污水中抗生素耐药菌(ARB)的去除,通过逐一分析不同频率超声波(33, 120, 200kHz)、不同波长UV-LED(255, 275nm)对耐四环素大肠杆菌的灭活以及膜损伤效能,从而确定超声波联合UV的工艺参数.结果表明,单独超声波作用时,33kHz超声波效果最佳,在输入功率为50W、输入剂量为1080kJ/L时,大肠杆菌的灭活率为2.30log,细胞膜损伤百分比为61.09%;单独UV-LED作用下,辐照剂量为19.92mJ/cm2的255nm UV-LED对细菌的灭活率为5.32log,细胞膜的损伤百分比为2.52%;275nm UV-LED对细菌的灭活率为4.63log,膜损伤百分比为34.95%;但是二者联合使用未产生协同效应.超声波联合UV-LED可使消毒效能显著提升至7.45log, 细胞膜损伤提高至68.74%,且能够有效抑制大肠杆菌的复活,复活率降低至0.4%,超声波联合UV-LED在细菌耐药性控制方面显现出巨大的潜力.
Abstract
In this study, the inactivation efficiency as well as membrane damage of tetracycline-resistant Escherichia coli under ultrasound at different frequencies (33, 120, 200kHz) and different UV-LED wavelengths (255, 275nm) were evaluated respectively, and furthermore, the optimized operation parameter were selected to form combined ultrasound and UV-LED process to control ARB in sewage effectively. The results showed that 33 kHz ultrasound alone was effective in ARB inactivation, and with an input power of 50 W accumulated to an input dose of 1080 KJ/L, the inactivation rate of Escherichia coli can reach to 2.30 log, while the percentage of cell membrane damage was 61.09%. For UV-LED alone, when 255nm UV-LED irradiated at dosage of 19.92mJ/cm2, the inactivation rate of bacteria and the percentage of cell membrane damage were 5.32log and 2.52% respectively. While the inactivation rate and the percentage of cell membrane damage were 4.63 log and 34.95% respectively for 275nm UV-LED irradiation, but dual-wavelength UV-LED irradiation did not show synergistic effect in bacteria inactivation. However, ultrasound combined with UV-LED can obviously improve the disinfection efficiency, the inactivation rate increased to be 7.45log, the cell membrane damage increased to be 68.74%. Meanwhile, the photoreactivation rate of bacteria after disinfection was reduced to 0.4%. The combination of ultrasound and UV-LED shows great potential in ARB control during wastewater treatment.
关键词
UV-LED /
超声波 /
光复活 /
抗性细菌 /
灭活效能
Key words
inactivation efficiency /
photoreactivation /
resistant bacteria /
ultrasound /
UV-LED
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基金
国家重点研发计划(2021YFC3201305);广东省环境污染控制与修复技术重点实验室开放基金资助项目(2020B1212060022)
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