Reduction of ARB and ARGs by ultraviolet, ozone and combined disinfection technology
LI Shu-ming1, WANG Jin1, WANG Hai-chao1, LI Jin-zhao2, HUANG Xue1, WANG Xiao-yue1
1. Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Civil Engineering, Beijing Jiaotong University, Beijing 100044, China;
2. China Shenhua Overseas Development & Investment Co. Limited, Beijing 100011, China
Four kinds of resistant bacterium Enterococcus, Brevibacillus, Pseudomonas, and Escherichia coli were isolated from the secondary effluent of a sewage treatment plant. The reduction effect of three disinfection methods of UV, O3 and UV/O3 on ARB and ARGs was evaluated by colony counting method and qPCR. The study showed that the three methods had obvious inactivation of ARB, and the effect on inactivation of Gram-positive bacteria was worse than that of Gram-negative bacteria. The reduction of ARGs varied by the means of disinfection and the types of ARGs, and the result indicated that Brevibacillus showed stronger resistance during the reduction process of ARB and ARGs. In addition, combined with photoreactivation experiments and scanning electron microscopy, it was found that the photoreactivation rate of ARB after UV disinfection for 72h was between 6.93% and 11.60%. There was no photoreactivation phenomenon by O3 due to the destruction of cell structure, which might lead intracellular ARGs to be released into the environment. Meanwhile, the ARB damage caused by UV/O3 was also irreversible.
李树铭, 王锦, 王海潮, 李今朝, 黄雪, 王晓月. UV、O3及UV/O3削减耐药菌和抗性基因性能[J]. 中国环境科学, 2019, 39(12): 5145-5153.
LI Shu-ming, WANG Jin, WANG Hai-chao, LI Jin-zhao, HUANG Xue, WANG Xiao-yue. Reduction of ARB and ARGs by ultraviolet, ozone and combined disinfection technology. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(12): 5145-5153.
Pruden A, Pei R, Storteboom H, et al. Antibiotic resistance genes as emerging contaminants:Studies in northern Colorado[J]. Environmental Science & Technology, 2006,40(23):7445-7450.
罗义,周启星.抗生素抗性基因(ARGs)——一种新型环境污染物[J]. 环境科学学报, 2008,28(8):1499-1505. Luo Y, Zhou Q X. Antibiotic resistance genes (ARGs) as emerging pollutants[J]. Acta Scientiae Circumstantiae, 2008,28(8):1499-1505.
罗晓,袁立霞,张文丽,等.制药废水厂抗性基因和微生物群落相关性研究[J]. 中国环境科学, 2019,39(2):831-838. Luo X, Yuan L X, Zhang W L, et al. Correlation study between resistance genes and microbial communities in pharmaceutical wastewater treatment plants[J]. China Environmental Science, 2019, 39(2):831-838.
张宁,李淼,刘翔.土壤中抗生素抗性基因的分布及迁移转化[J]. 中国环境科学, 2018,38(7):2609-2617. Zhang N, Li M, Liu X. Distribution and transformation of antibiotic resistance genes in soil[J]. China Environmental Science, 2018,38(7):2609-2617.
World Health Organization, Antimicrobial Resistance, (2015) Fact Sheet n 194.2014.
Mao D, Yu S, Rysz M, et al. Prevalence and proliferation of antibiotic resistance genes in two municipal wastewater treatment plants[J]. Water Research. 2015,85:458-466.
马奔,黄雅梦,王若楠,等.城市污水厂MCR-1基因及其携带菌的污染[J]. 中国环境科学, 2018,38(4):1433-1440. Ma B, Huang Y M, Wang R N, et al. The pollution of MCR-1and MCR-1hosting bacteria in municipal wastewater treatment plants[J]. China Environmental Science, 2018,38(4):1433-1440.
Yoon Y, Chung H J, Wen Di D Y, et al. Inactivation efficiency of plasmid-encoded antibiotic resistance genes during water treatment with chlorine, UV, and UV/H2O2[J]. Water Research, 2017,123:783-793.
Huang J, Hu H, Tang F, et al. Inactivation and reactivation of antibiotic-resistant bacteria by chlorination in secondary effluents of a municipal wastewater treatment plant[J]. Water Research, 2011,45(9):2775-2781.
Mckinney C W, Pruden A. Ultraviolet disinfection of antibiotic resistant bacteria and their antibiotic resistance genes in water and wastewater[J]. Environmental Science & Technology, 2012,46(24):13393-13400.
Sullivan B A, Vance C C, Gentry T J, et al. Effects of chlorination and ultraviolet light on environmental tetracycline-resistant bacteria and tet(W) in water[J]. Journal of Environmental Chemical Engineering, 2017,5(1):777-784.
Pak G, Salcedo D E, Lee H, et al. Comparison of antibiotic resistance removal efficiencies using ozone disinfection under different pH and suspended solids and humic substance concentrations[J]. Environmental Science & Technology, 2016,50(14):7590-7600.
曾萍,刘诗月,张俊珂,等.芬顿法深度处理生物处理排水中的四环素抗性基因[J]. 中国环境科学, 2017,37(9):3315-3323. Zeng P, Liu S Y, Zhang J K, et al. Advanced Fenton oxidation treatment of tetracycline resistance genes in effluent discharged from biological wastewater treatment[J]. China Environmental Science, 2017,37(9):3315-3323.
Fang J, Liu H, Shang C, et al. E.coli and bacteriophage MS2 disinfection by UV, ozone and the combined UV and ozone processes[J]. Frontiers of Environmental Science & Engineering. 2014,8(4):547-552.
Alleron L, Khemiri A, Koubar M, et al. VBNC Legionella pneumophila cells are still able to produce virulence proteins[J]. Water Research, 2013,47(17):6606-6617.
Lin Y, Li D, Gu A Z, et al. Bacterial regrowth in water reclamation and distribution systems revealed by viable bacterial detection assays[J]. Chemosphere, 2016,144:2165-2174.
钱雪琴,张军,沈芳.Chelex-100法和碱性裂解法提取细菌DNA的比较[J]. 中国卫生检验杂志, 2008,18(8):1565-1566. Qian X Q, Zhang J, Shen F. Comparison of methods for extraction of DNA by chelex-100 and alkali split[J]. Chinese Journal of Health Laboratory Technology, 2008,18(8):1565-1566.
胡晓红,彭惠民,刘昕,等. PCR及Real-time PCR评价细菌DNA提取方法[J]. 重庆医科大学学报, 2008,33(2):155-158. Hu X H, Peng H M, Liu X, et al. Methods of DNA extraction from bacteria for PCR and Real-time PCR[J]. Journal of Chongqing Medical University. 2008,33(2):155-158.
Singh U A, Kumari M, Iyengar S. Method for improving the quality of genomic DNA obtained from minute quantities of tissue and blood samples using Chelex 100resin[J]. Biological Procedures Online, 2018,20(12):1-8.
Zhang X, Zhang T. Occurrence, abundance, and diversity of tetracycline resistance genes in 15sewage treatment plants across China and other global locations[J]. Environmental Science & Technology, 2011,45(7):2598-2604.
Luo Y, Mao D, Rysz M, et al. Trends in antibiotic resistance genes occurrence in the Haihe River, China[J]. Environmental Science & Technology, 2010,44(19):7220-7225.
Zhu Y, Johnson T A, Su J, et al. Diverse and abundant antibiotic resistance genes in Chinese swine farms[J]. Proceedings of the National Academy of Sciences, 2013,110(9):3435-3440.
Goldstein C, Lee M D, Sanchez S, et al. Incidence of class 1and 2integrases in clinical and commensal bacteria from livestock, companion animals, and exotics[J]. Antimicrobial Agents and Chemotherapy, 2001,45(3):723-726.
郑吉,周振超,陈芳,等.3种常规消毒方法对磺胺类抗性基因削减效果的比较[J]. 环境科学, 2017,38(4):1497-1505. Zheng J, Zhou Z C, Chen F, et al. Reducing effect of three disinfection technologies for sulfonamides resistance genes[J]. Environmental Science, 2017,38(4):1497-1505.
Nicholson W L. Photoreactivation in the genus Bacillus[J]. Curr Microbiol. 1995,31(6):361-364.
Sousa J M, Macedo G, Pedrosa M, et al. Ozonation and UV254nm radiation for the removal of microorganisms and antibiotic resistance genes from urban wastewater[J]. Journal of Hazardous Materials, 2017,323:434-441.
Lee O, Kim H Y, Park W, et al. A comparative study of disinfection efficiency and regrowth control of microorganism in secondary wastewater effluent using UV, ozone, and ionizing irradiation process[J]. Journal of Hazardous Materials, 2015,295:201-208.
Bustos-Terrones Y, Rangel-Peraza J G, Sanhouse A, et al. Degradation of organic matter from wastewater using advanced primary treatment by O3 and O3/UV in a pilot plant[J]. Physics and Chemistry of the Earth, Parts A/B/C. 2016,91:61-67.
Zheng J, Su C, Zhou J, et al. Effects and mechanisms of ultraviolet, chlorination, and ozone disinfection on antibiotic resistance genes in secondary effluents of municipal wastewater treatment plants[J]. Chemical Engineering Journal, 2017,317:309-316.
Hall R M, Collis C M, Kim M J, et al. Mobile gene cassettes and integrons in evolution[J]. Annals of the New York Academy of Sciences, 1999,870:68-80.
Li G, Wang W, Huo Z, et al. Comparison of UV-LED and low pressure UV for water disinfection:Photoreactivation and dark repair of Escherichia coli[J]. Water Research, 2017,126:134-143.
Guo M, Hu H, Bolton J R, et al. Comparison of low-and medium-pressure ultraviolet lamps:Photoreactivation of Escherichia coli and total coliforms in secondary effluents of municipal wastewater treatment plants[J]. Water Research, 2009,43(3):815-821.
Xu L, Zhang C, Xu P, et al. Mechanisms of ultraviolet disinfection and chlorination of Escherichia coli:Culturability, membrane permeability, metabolism, and genetic damage[J]. Journal of Environmental Sciences, 2018,65:356-366.
Cho M, Kim J, Kim J Y, et al. Mechanisms of Escherichia coli inactivation by several disinfectants[J]. Water Research, 2010,44(11):3410-3418.
张崇淼,庄凯,巨欣,等.紫外线消毒对3种大肠杆菌的灭活效果和耐药性影响[J]. 环境工程学报, 2015,9(9):4097-4101. Zhang C M, Zhuang K, Ju X, et al. Inactivation and antibiotic resistance variation of three Escherichia coli under ultraviolet disinfection[J]. Chinese Journal of Environmental Engineering, 2015, 9(9):4097-4101.