A<sup>2</sup>/O工艺中溶解性有机氮的分子转化与生物有效性特征

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摘要本研究探究了厌氧/缺氧/好氧(A²/O)工艺沿程溶解性有机氮(DON)浓度分布及其分子转化规律,并运用多种方法表征了各单元污水DON的生物有效性特征.结果表明,DON浓度由进水(5.3±0.3)mg/L沿厌氧池、缺氧池分别下降至(2.0±0.1),(1.8±0.2) mg/L,而有意思的是好氧池中DON浓度反而升高至(1.9±0.1)mg/L,去除率竟为(-5.6±0.6)%.傅里叶变换离子回旋共振质谱(FT-ICR-MS)分析DON分子组成特征显示,各单元活性DON分子(H/C≥1.5)占比为:厌氧池(38%)<缺氧池(41%)<好氧池(42%),表明好氧池出水DON最不稳定,易被受纳水体中藻类微生物摄取利用.同时在各单元产生的DON分子中,以蛋白/氨基糖类化合物为特征的微生物源DON占比沿程上升,由厌氧池24.77%分别上升至缺氧池29.12%、好氧池33.11%,表明微生物源DON分子是导致好氧池出水DON生物有效性升高的原因.进一步地,采用藻类生物测定厌氧池、缺氧池和好氧池沿程DON生物有效性,分别为(33.2±3.0)%、(34.9±7.0)%和(42.1±4.0)%,该结果证实了FT-ICR-MS的测定结论.此外,主成分分析表明,相比于厌氧池和缺氧池,温度对好氧池DON分子组成具有显著影响,且冬季好氧池出水DON生物有效性(49.3%±2%)高于夏季(42.1%±4.0%).因此,冬季好氧池出水DON具有更高的富营养化潜在风险.

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	韩成龙
	赵凌云
	段冰
	崔贤
	刘彩风
	胡海冬
	任洪强

关键词 ：溶解性有机氮(DON), 生物有效性, A/A/O工艺, 傅里叶变换离子回旋共振质谱(FT-ICR-MS), 分子转化

Abstract：The concentration distribution, molecular transformation, and bioavailability characteristics of DON along the anaerobic/anoxic/oxic (A²/O) process were investigated by various methods. The results showed that the concentration of DON decreased from (5.3±0.3)mg/L in the influent to (2.0±0.1)mg/L and (1.8±0.2)mg/L along the anaerobic and anoxic tanks respectively. Interestingly, the concentration of DON in the oxic unit increased to (1.9±0.1)mg/L, and the removal rate was (-5.6±0.6)%. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) analysis of the molecular composition of DON showed that the proportion of active DON molecules (H/C≥1.5) in each unit was: anaerobic unit (38%)

Key words： dissolved organic nitrogen (DON) bioavailability anaerobic/anoxic/oxic process Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) molecular transformation

收稿日期: 2022-08-26

PACS:

X703

基金资助:国家自然科学基金资助项目(51808281);国家重点实验室导向性合作研究基金项目(PCRR-ZZ-202104)

通讯作者: 胡海冬,副教授,hdhu@nju.edu.cn E-mail: hdhu@nju.edu.cn

作者简介: 韩成龙(2000-),男,陕西商洛人,南京大学硕士研究生,主要研究方向为废水深度脱氮.181830065@smail.nju.edu.cn.

引用本文:

韩成龙, 赵凌云, 段冰, 崔贤, 刘彩风, 胡海冬, 任洪强. A²/O工艺中溶解性有机氮的分子转化与生物有效性特征[J]. 中国环境科学, 2023, 43(4): 1610-1619. HAN Cheng-long, ZHAO Ling-yun, DUAN Bing, CUI Xian, LIU Cai-feng, HU Hai-dong, REN Hong-qiang. Characterization of molecular transformation and bioavailability of dissolved organic nitrogen in the A/A/O process. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1610-1619.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I4/1610

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