C/N对HN-AD菌藻颗粒污泥体系处理农村污水的影响

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摘要通过在传统菌-藻共生好氧颗粒污泥体系(ABGS)中引入异养硝化-好氧反硝化(HN-AD)菌构建H-ABGS体系,以合成农村污水为研究对象,考察碳氮比(C/N=1,2,4,6,8,10)对H-ABGS系统中污染物去除性能和微生物特性影响.结果表明,C/N为4时,H-ABGS系统的总氮去除率高于ABGS系统(12.05%)和好氧颗粒污泥(AGS)系统(44.86%).微生物群落分析显示,适应低碳环境的Thauera菌属和脱氨关键菌属norank_f__A4b可能是确保H-ABGS系统在低碳环境下具备高脱氮性能的关键.微生物群落的共生模式和相关性分析表明,引入HN-AD菌有助于最小化生态位,与藻共同形成稳定的共生体系,从而确保系统的稳定性.此外, C/N为4时,高表达的硝酸还原酶可能有助于确保H-ABGS系统在低碳环境下具备卓越的脱氮性能.

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关键词 ：菌藻共生, HN-AD菌, 微生物群落, 脱氮

Abstract：The H-ABGS system was proposed by introducing heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria into the traditional algal-bacterial aerobic granular sludge (ABGS) system. The effects of carbon to nitrogen (C/N) ratios (C/N=1,2,4,6,8,10) on the pollutant removal performance and microbial characteristics of the H-ABGS system for synthetic rural wastewater treatment were investigated. The results showed that the total nitrogen (TN) removal rate of the H-ABGS system was higher than that of the ABGS system (12.05%) and AGS system (44.86%) at a C/N ratio of 4. The microbial community analysis showed that Thauera bacteria and norank_f__A4b bacteria, which were adapted to the low carbon environment, might be the key to ensuring the high nitrogen removal performance of the H-ABGS system under low carbon condition. The symbiosis pattern and correlation analysis of microbial communities showed that the inoculation of HN-AD bacteria could help to minimize the ecological niche and form a stable symbiotic system with algae, thus ensuring the stability of the system. In addition, the high expression of nitrate reductase at a C/N ratio of 4might help to ensure the excellent nitrogen removal performance of the H-ABGS system.

Key words： algae-bacteria symbiosis heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria microbial community nitrogen removal

收稿日期: 2023-07-14

PACS:

X703.5

基金资助:重庆市科技创新与应用示范项目(cstc2018jscx-mszdX0005);重庆市教育委员会科技研究计划项目(KJQN202001114);重庆市巴南区项目(2020QC368);重庆市自然科学基金项目(CSTB2023NSCQ- MSX0412);重庆理工大学研究生教育高质量发展项目(gzltd202304);重庆理工大学研究与创新团队培育计划项目(2023TDZ009)

作者简介: 刘欢(1999-),男,重庆江津人,重庆理工大学硕士研究生,主要从事HN-AD菌的应用与机理研究.发表论文2篇.15223242847@163.com.

引用本文:

刘欢, 杨晨曦, 秦树敏, 龙昆, 赵婷婷, 张千. C/N对HN-AD菌藻颗粒污泥体系处理农村污水的影响[J]. 中国环境科学, 2024, 44(3): 1296-1306. LIU Huan, YANG Chen-xi, QIN Shu-min, LONG Kun, ZHAO Ting-ting, ZHANG Qian. Effects of C/N on the treatment of rural wastewater by HN-AD bacterial-algal granular sludge system. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(3): 1296-1306.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2024/V44/I3/1296

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