不同补料方式对好氧颗粒污泥合成PHA的影响

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摘要为提高好氧颗粒污泥(AGS)合成聚羟基脂肪酸酯(PHA)的产量,探究了4种不同补料方式(未补料、好氧0h补料、好氧0.5h补料和好氧1h补料)对AGS合成PHA的影响,同时考察了此过程中AGS的碳氮磷去除效能以及微生物群落结构变化.结果表明,补料能够提高AGS合成PHA的含量,在好氧1h补料时AGS合成PHA的含量最高,为125.06mg/g;同时补料时间会影响AGS的脱氮除磷性能,当补料时间由好氧0h延迟至好氧1h时,AGS的总氮去除率由(87.49±5.49)%降至(67.60±16.24)%,溶解性正磷酸盐(SOP)去除率由(94.08±1.42)%降至(62.91±15.33)%.且不同补料时间条件下,AGS的微生物群落结构变化较大,合成PHA的优势菌群差异较大,在未补料和好氧0h补料条件下,优势菌为既能合成PHA同时能够脱氮或除磷的不动杆菌属(Acinetobacter);在好氧1h补料条件下中,优势菌为能合成PHA的噬氢菌属(Hydrogenophaga)和黄杆菌属(Flavobacterium).

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关键词 ：好氧颗粒污泥, 聚羟基脂肪酸酯(PHA), 脱氮除磷, 补料时间, 影响

Abstract：To enhance the production of polyhydroxyalkanoates (PHA) by aerobic granular sludge (AGS), the impact of four different extra dosing strategies (no-dosing, aerobic 0h-dosing, aerobic 0.5h-dosing, and aerobic 1h-dosing) on the synthesis of PHA by AGS were investigated. Simultaneously, the removal efficiencies of chemical oxygen demand (COD), total nitrogen (TN) and solubility orthophosphate (SOP) by AGS, along with changes in the microbial community composition were examined. The results indicate that extra dosing of organic carbon positively influenced PHA production, with the highest yield (125.06mg/g) observed under aerobic 1h-dosing condition. Additionally, the nutrient removal efficiency of AGS was influenced by dosing time. When dosing was postponed from aerobic 0h to 1h, TN removal decreased from (87.49 ± 5.49)% to (67.60 ± 16.24)%, and the removal efficiency of SOP decreased from (94.08 ± 1.42)% to (62.91 ± 15.33)%. Notably, under various dosing conditions, the microbial community structure of AGS exhibited significant variation. The dominant bacteria under no-dosing and aerobic 0h-dosing conditions were Acinetobacter, capable of both PHA production and nutrients removal. Conversely, while under aerobic 1h-dosing conditions, Hydrogenophaga and Flavobacterium were the dominant bacteria, specializing in PHA production.

Key words： aerobic granular sludge PHA nitrogen and phosphorus removal dosing time effect

收稿日期: 2023-07-19

PACS:

X703.5

基金资助:自然科学基金委资助项目(22278007)

作者简介: 陈淞(2000-),女,河北唐山人,北京工商大学硕士研究生,主要从事好氧颗粒污泥合成PHA的研究.chensong0630@hotmail.com.

引用本文:

陈淞, 苑泉, 娄雨晴, 张艳萍, 孙迎雪. 不同补料方式对好氧颗粒污泥合成PHA的影响[J]. 中国环境科学, 2024, 44(3): 1286-1295. CHEN Song, YUAN Quan, LOU Yu-qing, ZHANG Yan-ping, SUN Ying-xue. Effect of different extra dosing methods on the production of PHA from aerobic granular sludge. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(3): 1286-1295.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2024/V44/I3/1286

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