以预脱碳污水和市政污水作为目标污水,建立了好氧段分段进水的新模式,通过调整进水分配比来优化好氧段C/N,以期实现短程硝化-厌氧氨氧化(PN/A)工艺在实际工况下的稳定运行.采用4组以好氧/缺氧/好氧/缺氧(O/A/O/A)模式运行的序批式反应器(SBR),R1、R2、R3及R4以进水分配比(预脱碳污水:市政污水)分别为1:0、2:1、1:1及1:2的分段进水模式运行.结果表明,R1未能实现PN/A工艺,总氮去除率(NRE)仅为60.2%.随着进水分配比不断下降,R2、R3、R4分别在第34,30及36d实现PN/A工艺,NRE分别达到71.8%、80.3%及74.1%.其中R3系统处理性能最好且最稳定,厌氧氨氧化贡献率达到83.9%.污泥沉降性与粒径最佳.此外,高通量结果显示氨氧化菌(Nitrosomonas)和厌氧氨氧化菌(Candidatus_Kuenenia)丰度分别为2.74%和12.17%,且与好氧异养菌(AHB)形成了良好的协同作用.因此,分段进水模式在进水分配比为1:1时有利于实现稳定的PN/A工艺,此时各功能微生物可得到较好富集与平衡.
Abstract
In this work, a novel model was established to optimize the C/N in the aerobic section based on the regulation of the influent distribution ratio of each aerobic section, using pre-decarbonized wastewater and municipal wastewater as target wastewater. This work was aiming at achieving the stable operation of the partial nitrification-anammox (PN/A) process under realistic conditions. Four groups of sequential batch reactors (SBR) with the same specifications run in oxic/anoxic/oxic/anoxic (O/A/O/A) mode, R1, R2, R3, and R4 operated in the step-feed mode with the influent distribution ratios (pre-decarbonized wastewater: municipal wastewater) of 1:0, 2:1, 1:1, and 1:2. The results illustrated that R1failed to realize the PN/A process and total nitrogen removal efficiency (NRE) was only 60.2%. Along with the decreasing influent distribution ratio, R2, R3, and R4 completed the PN/A process on the 34th, 30th, and 36th days, which NRE reaching 71.8%, 80.3%, and 74.1%. Among them, R3 had the optimized performance with best stability, and the contribution of anammox reaching 83.9%. It was revealed that sludge exhibited excellent settling characteristics and optimal particle size. Moreover, high-throughput data also demonstrated the abundance of ammonia-oxidizing bacteria (Nitrosomonas) and anammox bacteria (Candidatus_Kuenenia) at 2.74% and 12.17%. These microbial populations synergized effectively with aerobic heterotrophic bacteria (AHB). Hence, the step-feed model could be conductive to achieve a stable PN/A process. The influent distribution ratio of 1:1was suggested as a suitable reference value, fostering better enrichment and balance of functional microorganisms.
关键词
PN/A工艺 /
分段进水 /
进水分配比 /
竞争性抑制
Key words
competitive inhibition /
influent distribution ratio /
PN/A process /
step-feed
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基金
北京高校卓越青年科学家计划项目(BJJWZYJH 01201910005019)
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