The effect and mechanism of dielectric barrier discharge combined with pulse corona discharge to inactivate of Aeromonas veronii in water
ZHU Zhen-fei, YAN Peng-cheng, ZHANG Rong, ZHANG Min, QU Guang-zhou
ZHU Zhen-fei, YAN Peng-cheng, ZHANG Rong, ZHANG Min, QU Guang-zhou* (College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China
Abstract：In this study, the DBD combined with PCD (DBD/PCD) was used to inactivate Aeromonas veronii in water. The synergistic effect of DBD and PCD was analyzed, and the effects of voltage, pH, carrier gas type and flow rate on the inactivation efficiency of Aeromonas veronii were investigated. The inactivation mechanism of Aeromonas veronii was explored, The types and effects of active substances were analyzed, and the safety of the DBD/PCD was verified through the experiment of zebrafish challenge. The results showed that the inactivation effect of DBD/PCD to Aeromonas veronii was significantly higher than that of DBD and PCD alone, and their synergistic coefficients were both greater than 1. After 420s of treatment, DBD/PCD can reduce the concentration of Aeromonas veronii by 7 orders of magnitude; Increasing the discharge voltage can significantly improve the inactivation efficiency of Aeromonas veronii; Under alkaline conditions, oxygen as a carrier gas is beneficial for the inactivation of Aeromonas veronii; The effect of air flow rate on inactivation efficiency is not obvious in the range of 0.5~1.5L/min. The active substances produced in the DBD/PCD system first act on the outer membrane of Aeromonas veronii cells, causing them to rupture, and then enter the cells to cause damage to intracellular proteins and the virulence genes, ultimately leading to the inactivation of Aeromonas veronii. Active substances (·OH, 1O2 and O2-) produced by DBD/PCD system play an important role in the inactivation of Aeromonas veronii. The experiment of zebrafish challenge showed that the DBD/PCD is a safe and efficient method to inactivate pathogenic microorganisms in water bodies.
[1] 倪盈,陈永昌,王明连,等.低温等离子体杀菌的实验研究[J]. 环境工程学报, 2009,3(11):1951-1955. Ni Y, Chen YC, Wang ML, et al. Experimental study on low-temperature plasma sterilization [J]. Journal of Environmental Engineering, 2009,3(11):1951-1955. [2] 马可可,周律,辛怡颖,等.低温等离子体技术用于废水处理的研究进展[J]. 应用化工, 2019,48(1):145-150. Ma KK, Zhou L, Xin YY, et al. Research progress of low-temperature plasma technology for wastewater treatment [J]. Applied Chemical Industry, 2019,48(1):145-150. [3] 张禾,陈烨芝,孙翠,等.低温等离子体对杨梅采后致病菌——桔青霉的抑制作用[J]. 中国食品学报, 2022,22(7):183-192. Zhang H, Chen Y Z, Sun C, et al. The inhibitory effect of low-temperature plasma on the postharvest pathogenic bacteria of Yangmei, Penicillium citri [J]. Chinese Journal of Food Science, 2022, 22(7):183-192. [4] 方志,邱毓昌,王辉,等.介质阻挡放电的电荷传输特性研究[J]. 高压电, 2004,40(6):401-403. Fang Z, Qiu YC, Wang H, et al. Study on Charge Transport Characteristics of Dielectric Barrier Discharge [J]. High Voltage Electricity, 2004,40(6):401-403. [5] 王伟,王永刚,吴忠航,等.脉冲驱动氩气真空介质阻挡放电的光谱特性研究[J]. 光谱学与光谱分析, 2023,43(2):455-459. Wang W, Wang YG, Wu ZH, et al. Spectral characteristics of pulse driven argon vacuum dielectric barrier discharge [J]. Spectroscopy and Spectral Analysis, 2023,43(2):455-459. [6] 欧阳吉庭,张晨阳,张宇,等.电晕放电的杀菌机理研究[J]. 北京理工大学学报, 2020,40:908-913. Ou Y J T, Zhang C Y, Zhang Y, et al. Research on the bactericidal mechanism of corona discharge [J]. Journal of Beijing Institute of Technology, 2020,40:908-913. [7] 董晓宇.大气压冷等离子体对Klebsiella pneumoniae灭菌机制和诱变研究[D]. 大连:大连理工大学, 2010.Dong X Y. Study on the sterilization mechanism and mutagenesis of Klebsiella pneumoniae by atmospheric pressure-cooled plasma [D]. Dalian: Dalian University of Technology, 2010. [8] Joubert V, Cheype C, Bonnet J, et al. Inactivation of Bacillus subtilis var. niger of both spore and vegetative forms by means of corona discharges applied in water [J]. Water Research, 2013,47(3):1381-1389. [9] Wang H, Qu GZ, Gan YS, et al. Elimination of Microcystis aeruginosa in water via dielectric barrier discharge plasma: Efficacy, mechanism and toxin release [J]. Journal of Hazardous Materials, 2022,422: 126956. [10] Song KG, Wang H, Jiao Z, et al. Inactivation efficacy and mechanism of pulsed corona discharge plasma on virus in water [J]. Journal of Hazardous Materials, 2022,422:126906. [11] Zhang H, Ma J, Shen J, et al. Roles of membrane protein damage and intracellular protein damage in death of bacteria induced by atmospheric-pressure air discharge plasmas [J]. RSC Advances, 2018,8(38):21139-21149. [12] 杨德敏,张烨,袁建梅,等.功能陶瓷膜催化臭氧氧化处理页岩气压裂废水[J]. 水处理技术, 2023,49(10):122-125,140. Yang DM, Zhang Y, Yuan JM, et al. Functional Ceramic Membrane Catalytic Ozone Oxidation for Shale Gas Fracturing Wastewater [J]. Water Treatment Technology, 2023,49(10):122-125,140. [13] Toyokawa Y, Yagyu Y, Misawa T, et al. A new roller conveyer system of non-thermal gas plasma as a potential control measure of plant pathogenic bacteria in primary food production [J]. Food Control, 2017,72:62-72. [14] 吴伟全,王思捷,李元歌,等.激光扫描共聚焦显微镜及透射电镜观察肺癌A549细胞亚细胞结构溶酶体的对比分析[J]. 临床医学工程, 2013,20(6):666-668. Wu WQ, Wang SJ, Li YG, et al. Comparative analysis of subcellular lysosomes in lung cancer A549cells using laser scanning confocal microscopy and transmission electron microscopy [J]. Clinical Medical Engineering, 2013,20(6):666-668. [15] He X S, Fan Q D. Investigating the effect of landfill leachates on the characteristics of dissolved organic matter in groundwater using excitation–emission matrix fluorescence spectra coupled with fluorescence regional integration and self-organizing map [J]. Environmental Science and Pollution Research, 2016,23:21229-21237. [16] Liu Y, Zhang WP, Wang Y, et al. Oxidative stress, intestinal damage, and cell apoptosis: Toxicity induced by fluopyram in Caenorhabditis elegans [J]. Chemosphere, 2022,286(P3):131830. [17] Murakawa M, Jung SK, Iijima K, et al. Apoptosis-inducing protein, AIP, from parasite-infected fish induces apoptosis in mammalian cells by two different molecular mechanisms [J]. Cell Death and Differentiation, 2001,8(3):298-307. [18] Zhang Y Z, Zhou B, Liu Y X, et al. Fluorescence study on the interaction of bovine serum albumin with p-aminoazobenzene [J]. Journal of Fluorescence, 2008,18(1):109-118. [19] Shi W X, Zhuang W E, Hur J, et al. Monitoring dissolved organic matter in wastewater and drinking water treatments using spectroscopic analysis and ultra-high resolution mass spectrometry [J]. Water Research, 2020,188:116406. [20] Fernández-Bravo A, Figueras M J. An update on the Genus Aeromonas: Taxonomy, epidemiology, and pathogenicity [J]. Microorganisms, 2020,8(1):129. [21] 齐燕玲,方琼亚,丁妍,等.鲫鱼致病性维氏气单胞菌的分离鉴定及生物学特性分析[J]. 黑龙江畜牧兽医, 2023,4:64-71,130-131. Qi Y L, Fang Q Y, Ding Y, et al. Isolation, identification, and biological characteristics analysis of pathogenic Pseudomonas aeruginosa in crucian carp [J]. Heilongjiang Animal Husbandry and Veterinary Medicine, 2023,4:64-71,130-131.
