莫莫格自然保护区水体DOM组成及其水质关联

郑珊珊; 刘东萍; 谢晓琳; 钱锋; 于会彬; 宋永会

PDF(2957 KB)

中国环境科学 ›› 2025, Vol. 45 ›› Issue (2) : 1016-1026.

环境生态

莫莫格自然保护区水体DOM组成及其水质关联

郑珊珊^1,2, 刘东萍², 谢晓琳², 钱锋², 于会彬², 宋永会²

作者信息 +

The composition of DOM and its correlation with water quality in Momoge Nature Reserve

ZHENG Shan-shan^1,2, LIU Dong-ping², XIE Xiao-lin², QIAN Feng², YU Hui-bin², SONG Yong-hui²

Author information +

文章历史 +

摘要

针对莫莫格自然保护区,选择3个水体,利用三维荧光光谱结合平行因子分析(PARAFAC)探究溶解性有机质(DOM)的组成、光谱特征和来源;采用二维相关光谱结合结构方程模型分析DOM组分变化及其与水质指标的响应关系.结果表明,水体DOM受内源和外源共同作用,腐殖化特征明显,蛋白质组分含量较低.PARAFAC共识别出微生物代谢产物(C1)、类富里酸(C2和C4)、类胡敏酸(C3)和类色氨酸(C5)等5种组分,其中C1组分含量最高(41.37%);DOM来源、组成、转化与保护区内水生动、植物生长环境下的微生物代谢过程紧密相关,采用2D-COS表征沿水流方向DOM组分的变化顺序为:C4→C2→C3→C1→C5,类富里酸(C2和C4)含量的变化幅度大于类色氨酸,类色氨酸的含量相对比较稳定.C1、C5组分与COD_Mn呈显著正相关,表明其具有同源性,主要来源于藻类生长死亡过程中产生的大量有机物.DOM来源和组成对水环境因子有直接或间接的影响,DOM主要通过腐殖化程度直接影响水质状态,影响权重为46.17%;DOM组分C2和C3间接影响水质状态,影响权重为17.59%.莫莫格自然保护区水体DOM组成与水环境因子响应机制的研究,可为自然保护区水生态保护提供理论依据.

Abstract

In Momoge Nature Reserve, three distinct water bodies were selected for the investigation of the composition, spectral characteristics, and sources of dissolved organic matter (DOM) utilizing three-dimensional excitation emission matrix spectroscopy in conjunction with parallel factor analysis (PARAFAC). Additionally, two-dimensional correlation spectroscopy combined with structural equation modeling was employed to analyze the variations of DOM components and their relationships with water quality parameters. The results indicated that the DOM in the water bodies originated from both endogenous and exogenous sources, which has obvious humification characteristics. Five components were identified as microbial metabolites (C1), fulvic acid-like (C2 and C4), humic acid-like (C3) and tryptophan-like (C5) through three-dimensional fluorescence coupled with PARAFAC. Of five components, C1content was the highest (41.37%). The changing sequence of DOM components along the direction of water flow was characterized by 2D-COS as follows: C4→C2→C3→C1→C5, with humic-like substances showing greater variation than tryptophan-like substances, and the content of tryptophan-like substances being relatively stable. The humification degree of DOM directly affected the water quality status with influence weight of 46.17%. The water quality was indirectly impacted by DOM components C2 and C3 with a 17.59% influence weight. Insight into the response mechanism of DOM properties to water quality in Momoge Nature Reserve could provide a theoretical basis for the ecological protection of nature reserves.

导出引用

郑珊珊, 刘东萍, 谢晓琳, 钱锋, 于会彬, 宋永会. 莫莫格自然保护区水体DOM组成及其水质关联[J]. 中国环境科学. 2025, 45(2): 1016-1026

ZHENG Shan-shan, LIU Dong-ping, XIE Xiao-lin, QIAN Feng, YU Hui-bin, SONG Yong-hui. The composition of DOM and its correlation with water quality in Momoge Nature Reserve[J]. China Environmental Science. 2025, 45(2): 1016-1026

中图分类号： X524

参考文献

[1] 于会彬,宋永会,杨楠,等.三维荧光与神经网络研究城市河流沉积物孔隙水有机物组成与结构特征 [J]. 光谱学与光谱分析, 2015, 35(4):934-939.Yu H B, Song Y H, Yang N, et al. Characterizing structural composition of dissolved and particulate organic matter from sediment pore water in a urban river using excitation-emission matrix fluorescence with self-organizing map [J]. Spectroscopy and Spectral Analysis, 2015,35(4):934-939.
[2] Herzsprung P, Tuempling W V, Hertkorn N, et al. Variations of DOM quality in inflows of a drinking water reservoir: linking of van Krevelen diagrams with EEMF spectra by rank correlation [J]. Environmental Science & Technology, 2012,46(10):5511-5518.
[3] Mladenov N, Sommaruga R, Morales-Baquero R, et al. Dust inputs and bacteria influence dissolved organic matter in clear alpine lakes [J]. Nature Communications, 2011,2:405.
[4] He Y, Song N, Jiang H L. Effects of dissolved organic matter leaching from macrophyte litter on black water events in shallow lakes [J]. Environmental Science and Pollution Research, 2018,25(10):9928-9939.
[5] Liu D, Du Y, Yu S, et al. Human activities determine quantity and composition of dissolved organic matter in lakes along the Yangtze River [J]. Water Research, 2020,168:115132.
[6] Borisover M, Laor Y, Parparov A, et al. Spatial and seasonal patterns of fluorescent organic matter in Lake Kinneret (Sea of Galilee) and its catchment basin [J]. Water Research, 2009,43(12):3104-3116.
[7] Xia X, Rabearisoa A H, Jiang X, et al. Bioaccumulation of perfluoroalkyl substances by Daphnia magna in water with different types and concentrations of protein [J]. Environmental Science & Technology, 2013,47(19):10955-10963.
[8] Hudson N, Baker A, Ward D, et al. Can fluorescence spectrometry be used as a surrogate for the Biochemical Oxygen Demand (BOD) test in water quality assessment? An example from South West England [J]. Science of the Total Environment, 2008,391(1):149-158.
[9] Osburn C L, Handsel L T, Mikan M P, et al. Fluorescence tracking of dissolved and particulate organic matter quality in a river-dominated estuary [J]. Environmental Science & Technology, 2012,46(16):8628-8636.
[10] Yu H B, Song Y H, Gao H J, et al. Applying fluorescence spectroscopy and multivariable analysis to characterize structural composition of dissolved organic matter and its correlation with water quality in an urban river [J]. Environmental Earth Sciences, 2015,73(9):5163-5171.
[11] Gao X T, Tan W B, Zhao Y, et al. Diversity in the mechanisms of humin formation during composting with different materials [J]. Environmental Science & Technology, 2019,53(7):3653-3662.
[12] Lee B M, Hur J. Adsorption behavior of extracellular polymeric substances on graphene materials explored by fluorescence spectroscopy and two-dimensional fourier transform infrared correlation spectroscopy [J]. Environmental Science & Technology, 2016,50(14):7364-7372.
[13] Node I. Two-dimensional correlation spectroscopy (2DCOS) analysis of polynomials [J]. Journal of Molecular Structure, 2016,1124:53-60.
[14] Zhu L J, Zhao Y, Bai S C, et al. New insights into the variation of dissolved organic matter components in different latitudinal lakes of northeast China [J]. Limnology and Oceanography, 2020,65(3):471-481.
[15] 邓远东,冶雪艳,吴亚敏,等.松嫩平原西部地下水氟和砷的富集机理与动态变化特征 [J]. 中国环境科学, 2023,43(10):5277-5290.Deng Y D, Ye X Y, Wu Y M, et al. Enrichment mechanism and dynamic variation characteristics of fluorine and arsenic in groundwater of western Songnen Plain [J]. China Environmental Science, 2023,43(10):5277-5290.
[16] 张洺也,齐清,佟守正,等.不同水文条件下莫莫格薹草草丘湿地土壤种子库特征及其与土壤环境的关系 [J]. 湿地科学, 2023,21(2): 239-247.Zhang M Y, Qi Q, Tong S Z, et al. Characteristics of soil seed banks and their relationships with soil factors in tussock wetland of the Momoge National Nature Reserve under different hydrological conditions [J]. Wetland Science, 2023,21(2):239-247.
[17] 倪鹤珊,李鸿雁,佟守正,等.基于生态环境需水量的莫莫格国家级自然保护区湿地生态服务功能评价 [J]. 湿地科学, 2024,22(3):437-444.Ni H S, Li H Y, Tong S Z, et al. Evaluation of wetland ecological service function in Momoge National Nature Reserve based on ecological water demand [J]. Wetland Science, 2024,22(3):437-444.
[18] 高纪超,朱平,李强,等.松嫩平原不同耕地土壤剖面的有机质及腐殖物质分布特征对比研究 [J]. 土壤与作物, 2024,13(2):165-172.Gao J C, Zhu P, Li Q, et al. Comparative study on the distribution of soil organic matter and humic substances in the profile of different cultivated soils in Songnen Plain [J]. Soils and Crops, 2024,13(2): 165-172.
[19] 杨富亿,文波龙,李晓宇,等.吉林莫莫格国家级自然保护区河流湿地的鱼类栖息地修复效果评价 [J]. 湿地科学, 2024,22(1):1-15.Yang F Y, Wen B L, Li X Y, et al. Restoration effect evaluation of fish habitats in river wetlands in Jilin Momoge National Nature Reserve [J]. Wetland Science, 2024,22(1):1-15.
[20] 国家环境保护总局.水和废水监测分析方法 [M]. 4版.北京:中国环境科学出版社, 2002.State Environmental Protection Admistration. Water and Wastewater Emonitoring and Xanalysis methods [M] 4th ed.Beijing: China Environmental Publishing Press, 2002.
[21] Zepp R G, Sheldon W M, Moran M A. Dissolved organic fluorophores in southeastern US coastal waters: correction method for eliminating Rayleigh and Raman scattering peaks in excitation-emission matrices [J]. Marine Chemistry, 2004,89(1):15-36.
[22] Murphy K R, Stedmon C A, Graeber D, et al. Fluorescence spectroscopy and multi-way techniques. PARAFAC [J]. Analytical Methods, 2013,5(23):6557-6566.
[23] Liu C, Li Z W, Berhe A A, et al. Characterizing dissolved organic matter in eroded sediments from a loess hilly catchment using fluorescence EEM-PARAFAC and UV-Visible absorption: Insights from source identification and carbon cycling [J]. Geoderma, 2019, 334:37-48.
[24] Yu G H, Tang Z, Xu Y C, et al. Multiple fluorescence labeling and two dimensional FTIR-13C NMR heterospectral correlation spectroscopy to characterize extracellular polymeric substances in biofilms produced during composting [J]. Environmental Science & Technology, 2011,45(21):9224-9231.
[25] Yu H B, Song Y H, Pan H W, et al. Synchronous fluorescence spectroscopy combined with two-dimensional correlation and principle component analysis to characterize dissolved organic matter in an urban river [J]. Environmental Monitoring and Assessment, 2016, 188(10):579.
[26] 金喆,孙晨,孔令昊,等.松嫩平原典型高氟区水库周边浅层地下水化学特征及高氟成因 [J]. 环境科学学报, 2023,43(12):250-258.Jin Z, Sun C, Kong L H, et al. Chemical characteristics and high-fluoride origins of shallow groundwater around typical high fluorine reservoir in Songnen Plain [J]. Acta Scientiae Circumstantiae, 2023, 43(12):250-258.
[27] Chen B F, Huang W, Ma S Z, et al. Characterization of chromophoric dissolved organic matter in the littoral zones of eutrophic Lakes Taihu and Hongze during the algal bloom season [J]. Water, 2018,10(7):861.
[28] Chen W, Westerhoff P, Leenheer J A, et al. Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter [J]. Environmental Science & Technology, 2003,37(24): 5701-5710.
[29] Coble P G. Characterization of marine and terrestrial DOM in seawater using excitation-emission matrix spectroscopy [J]. Marine Chemistry, 1996,51(4):325-346.
[30] Li W T, Xu Z X, Li A M, et al. HPLC/HPSEC-FLD with multi-excitation/emission scan for EEM interpretation and dissolved organic matter analysis [J]. Water Research, 2013,47(3):1246-1256.
[31] Zhou X, Chen Z, Li Z, et al. Impacts of aeration and biochar addition on extracellular polymeric substances and microbial communities in constructed wetlands for low C/N wastewater treatment: Implications for clogging [J]. Chemical Engineering Journal, 2020,396:125349.
[32] Yamin G, Borisover M, Cohen E, et al. Accumulation of humic-like and proteinaceous dissolved organic matter in zero-discharge aquaculture systems as revealed by fluorescence EEM spectroscopy [J]. Water Research, 2017,108:412-421.
[33] McKnight D M, Boyer E W, Westerhoff P K, et al. Spectrofluorometric characterization of dissolved organic matter for indication of precursor organic material and aromaticity [J]. Limnology and Oceanography, 2001,46(1):38-48.
[34] 蔡文良,许晓毅,罗固源,等.长江重庆段溶解性有机物的荧光特性分析 [J]. 环境化学, 2012,31(7):1003-1008.Cai W L, Xu X Y, Luo G Y, et al. Fluorescence characteristics of dissolved organic matter in the Chongqing section of Yangtze River [J]. Environmental Chemistry, 2012,31(7):1003-1008.
[35] 闫晓寒,韩璐,文威,等.辽河保护区水体溶解性有机质空间分布与来源解析 [J]. 环境科学学报, 2021,41(4):1419-1427.Yan X H, Han L, Wen W, et al. Spectral characteristics and spatial distribution of DOM in surface water of Liaohe reservation zone [J]. Acta Scientiae Circumstantiae, 2021,41(4):1419-1427.
[36] 张文浩,赵铎霖,王晓毓,等.太白山自然保护区水体CDOM吸收与三维荧光特征 [J]. 环境科学, 2020,41(11):4958-4969.Zhang W H, Zhao D L, Wang X Y, et al. Absorption and three dimensional fluorescence spectra of CDOM in the water of the Taibaishan Nature Reserve [J]. Environmental Science, 2020,41(11): 4958-4969.
[37] Fouché J, Christiansen C T, Lafrenière M J, et al. Canadian permafrost stores large pools of ammonium and optically distinct dissolved organic matter [J]. Nature Communications, 2020,11(1):4500.
[38] Stedmon C A, Markager S, Tranvik L, et al. Photochemical production of ammonium and transformation of dissolved organic matter in the Baltic Sea [J]. Marine Chemistry, 2007,104(3):227-240.
[39] Xu H, Guan D X, Zou L, et al. Contrasting effects of photochemical and microbial degradation on Cu(II) binding with fluorescent DOM from different origins [J]. Environmental Pollution, 2018,239:205-214.
[40] 庄奔,李冰,郭燕妮,等.洞庭湖DOM组成与有机碳收支对江湖水力连通的响应 [J]. 中国环境科学, 2024,44(3):1466-1475.Zhuang B, Li B, Guo Y N, et al. Response of DOM composition and organic carbon balance to the hydraulic connectivity of the Lake Dongting watershed [J]. China Environmental Science, 2024,44(3): 1466-1475.
[41] Gao Z Y, Gueguen C. Distribution of thiol, humic substances and colored dissolved organic matter during the 2015 Canadian Arctic GEOTRACES cruises [J]. Marine Chemistry, 2018,203:1-9.
[42] Murphy K R, Stedmon C A, Waite T D, et al. Distinguishing between terrestrial and autochthonous organic matter sources in marine environments using fluorescence spectroscopy [J]. Marine Chemistry, 2008,108(1):40-58.
[43] Wunsch U J, Geuer J K, Lechtenfeld O J, et al. Quantifying the impact of solid-phase extraction on chromophoric dissolved organic matter composition [J]. Marine Chemistry, 2018,207:33-41.
[44] Chen M L, Kim S H, Jung H J, et al. Dynamics of dissolved organic matter in riverine sediments affected by weir impoundments: Production, benthic flux, and environmental implications [J]. Water Research, 2017,121:150-161.
[45] Meilleur C, Kamula M, Kuzyk Z A, et al. Insights into surface circulation and mixing in James Bay and Hudson Bay from dissolved organic matter optical properties [J]. Journal of Marine Systems, 2023,238:103841.
[46] 朱爱菊,孙东耀,谭季,等.亚热带河口陆基养虾塘水体CDOM三维荧光光谱平行因子分析 [J]. 环境科学, 2019,40(1):164-171.Zhu A J, Sun D Y, Tan J, et al. Parallel factor analysis of fluorescence excitation emission matrix spectroscopy of CDOM from the mid-culture period of shrimp ponds in a subtropical estuary [J]. Environmental Science, 2019,40(1):164-171.
[47] Zepp R G, Sheldon W M, Moran M A. Dissolved organic fluorophores in southeastern US coastal waters: correction method for eliminating Rayleigh and Raman scattering peaks in excitation-emission matrices [J]. Marine Chemistry, 2004,89(1-4):15-36.
[48] 杨毅,董承璇,朱裕强,等.枯水期西安水体中DOM的组成、性质和来源 [J]. 中国环境科学, 2024,44(2):953-960.Yang Y, Dong C X, Zhu Y Q, et al. Components, properties and sources of DOM in Xi'an water bodies in dry season [J]. China Environmental Science, 2024,44(2):953-960.
[49] 孟永霞,程艳,李琳,等.匹里青河夏季有色溶解性有机质(CDOM)分布特征及来源分析 [J]. 环境化学, 2020,39(11):3213-3222.Meng Y X, Cheng Y, Li L, et al. Distribution characteristics and source analysis of chromophoric dissolved organic matter(CDOM) in Piliqing River in summer [J]. Environmental Chemistry, 2020,39(11):3213-3222.
[50] 冯可心,李永峰,姜霞,等.丹江口水库表层沉积物有色可溶性有机物空间分布特征及其来源分析 [J]. 环境化学, 2016,35(2):373-382.Feng K X, Li Y F, Jiang X, et al. Distribution and source analysis of chromophoric dissolved organic matter in the surface sediments of the Danjiangkou Reservoir [J]. Environmental Chemistry, 2016,35(2): 373-382.
[51] 陶勇,张瑾,张亚辉,等.临江河水体溶解性有机质荧光光谱特征解析 [J]. 农业环境科学学报, 2023,42(12):2711-2720.Tao Y, Zhang J, Zhang Y H, et al. Analysis of the fluorescence spectral characteristics of dissolved organic matter in the Linjiang River watershed [J]. Journal of Agro-Environment Science, 2023,42(12): 2711-2720.
[52] Lyu L L, Liu G, Shang Y X, et al. Characterization of dissolved organic matter (DOM) in an urbanized watershed using spectroscopic analysis [J]. Chemosphere, 2021,277:130210.