三峡水库消落带土壤反硝化及DOM的影响

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (3079 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要在重庆主城与涪陵选取了3个典型点位采集土壤样品,并对土壤的理化性质、原位反硝化速率、溶解性有机质(DOM)驱动的反硝化以及库区土壤的微生物进行了考察与分析.结果显示在海拔145~155m区间,淹水期镇安镇、涪陵区和鱼嘴镇最高氨氮(NH₄⁺-N)平均浓度分别为31.32,28.63和19.23mg/kg,均高于落干期.在淹水期,与高海拔区域土壤中硝酸盐氮(NO₃^--N)平均浓度相比,低海拔土壤中NO₃^--N平均浓度分别增大了46.91%(镇安镇)、37.89%(涪陵区)和29.69%(鱼嘴镇).在淹水期,土壤有机质(SOM)平均浓度随着海拔高程的降低而降低,镇安镇、涪陵区和鱼嘴镇分别从109.16,80.93和82.61mg/kg降至65.63,64.53,53.41mg/kg.土壤中NH₄⁺-N和NO₃^--N的含量与现场反硝化速率呈显著正相关(P<0.05),在提供充足的碳(C)和氮(N)营养元素后,土壤潜在反硝化速率表现出较大的时间和空间差异性.在由DOM驱动的反硝化实验中,DOM降解符合一级动力学模型(R²>0.93),N₂O累积量符合Logistic模型(R²>0.97).在三峡水库消落带土壤中,反硝化功能微生物主要是Bacillus和Comamonadaceae,其丰度变化与土壤潜在反硝化速率呈现显著的正相关性(P<0.05).

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	朱砚涛
	苏培兴
	张代钧
	袁淑培
	张峻通
	刘寅飞

关键词 ：三峡水库消落带, 土壤反硝化, 溶解性有机质, 微生物群落

Abstract：Soil samples were collected from three typical sites in the main city of Chongqing and Fuling, and the physical and chemical properties of soil, in-situ denitrification rate, DOM-driven denitrification process and soil micro-organisms community in the sampled sites were investigated and analyzed. The results showed that in the flooding period(145~155m), the average concentrations of the highest ammonia nitrogen (NH₄⁺-N) in Zhen'an, Fuling and Yuzui were 31.32, 28.63 and 19.23mg/kg, respectively, which were higher than those in the drying period. During the flooding period, compared with the average concentration of nitrate nitrogen (NO₃^--N) in the soil at high altitude, the average concentration of NO₃^--N in the soil at low altitude increased by 46.91 % (Zhen'an), 37.89 % (Fuling) and 29.69 % (Yuzui), respectively. During the flooding period, the average concentration of soil organic matter (SOM) decreased with the decrease of altitude, from 109.16,80.93 and 82.61mg/kg in Zhen'an, Fuling and Yuzui to 65.63,64.53 and 53.41mg/kg, respectively. The contents of NH₄⁺-N and NO₃^--N in the soil were significantly positively correlated with the in-situ denitrification rate (P<0.05). It was found that the potential denitrification rate of soil showed great temporal and spatial differences after given sufficient carbon (C) and nitrogen (N) nutrients. In the experiment of denitrification by using DOM as carbon source, the degradation of DOM conformed to the first-order kinetic model (R²>0.93), and the accumulation of N₂O conformed to the Logistic model (R²>0.97). The denitrifying microorganisms in the soil of WLFZ are mainly Bacillus and Comamonadaceae, and their abundance changes have a significant positive correlation with the potential denitrification rate of the soil (P<0.05).

Key words： water-level-fluctuation-zone of the Three Gorges Reservoir denitrification of soil dissolved organic matter microbial community

收稿日期: 2023-11-16

PACS:

X524

基金资助:重庆市自然科学基金资助重点项目(cstc2017jcyjBX0042)

通讯作者: 张代钧,教授,dzhang@cqu.edu.cn E-mail: dzhang@cqu.edu.cn

作者简介: 朱砚涛(1998-),男,云南建水人,重庆大学硕士研究生,主要研究方向为水污染控制.1711532972@qq.com.

引用本文:

朱砚涛, 苏培兴, 张代钧, 袁淑培, 张峻通, 刘寅飞. 三峡水库消落带土壤反硝化及DOM的影响[J]. 中国环境科学, 2024, 44(6): 3270-3279. ZHU Yan-tao, SU Pei-xing, ZHANG Dai-jun, YUAN Shu-pei, ZHANG Jun-tong, LIU Yin-fei. The characteristics of denitrification in soil and the effect of dissolved organic matter for the typical area of water-level-fluctuating zone of the Three Gorges Reservoir. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(6): 3270-3279.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2024/V44/I6/3270

[1] 徐建霞,王建柱.三峡库区香溪河消落带植被群落特征与土壤环境相关性[J].生态学杂志, 2018,37(12):3661-3669. Xu J X, Wang J Z. Correlation between vegetation community characteristics and soil physical-chemical factors in water-level fluctuation zone of Xiangxi River of the Three Gorges Reservoir area[J]. Chinese Journal of Ecology, 2018,37(12):3661-3669.
[2] Urbanič G, Politti E, Rodríguez-González P M, et al. Riparian Zones-from policy neglected to policy integrated[J]. Frontiers in environmental science, 2022,10:868527.
[3] Liu D, Nie Q, Xiong C M, et al. Sediment particle size composition in the Riparian Zone of the Three Gorges Reservoir[J]. Frontiers in Environmental Science, 2022,10.
[4] Pisani O, Bosch D D, Coffin A W, et al. Riparian land cover and hydrology influence stream dissolved organic matter composition in an agricultural watershed[J]. The Science of the Total Environment, 2020,717(May15):137161-137165.
[5] Nishisaka C S, Youngerman C, Meredith L K, et al. Differences in N₂O fluxes and denitrification gene abundance in the wet and dry seasons through soil and plant residue characteristics of tropical tree crops[J]. Frontiers in Environmental Science, 2019,doi:10.3389/fenvs. 2019.00011.
[6] Tatariw C, Chapman E L, Sponseller R A, et al. Denitrification in a large river:Consideration of geomorphic controls on microbial activity and community structure[J]. Ecology, 2013,94(10):2249-2262.
[7] Xiong Z, Li S, Yao L, et al. Topography and land use effects on spatial variability of soil denitrification and related soil properties in riparian wetlands[J]. Ecological Engineering, 2015,83:437-443.
[8] 韩晓丽,黄春国,张芸香,等.文峪河上游河岸带不同植被类型土壤nirS反硝化菌群结构及功能[J].生态学报, 2020,40(6):1977-1989. Han X L, Huang G C, Zhang Y X, et al. nirS-type denitrifiers community composition and function in different riparian vegetation types in upper Wenyuhe watershed[J]. Acta Ecologica Sinica, 2020, 40(6):1977-1989.
[9] Tomasek A A, Hondzo M, Kozarek J L, et al. Intermittent flooding of organic-rich soil promotes the formation of denitrification hot moments and hot spots[J]. Ecosphere, 2019,10(1):e02549.
[10] 徐硕,李玉双,魏建兵,等.河岸带土壤反硝化作用研究进展[J].生态科学, 2021,40(4):229-236. Xu S, Li Y S, Wei J B, et al. Research progress of riparian soil denitrification[J]. Ecological Science, 2021,40(4):229-236.
[11] Wang J, Fu B J, Qiu Y, et al. Soil nutrients in relation to land use and landscape position in the semi-arid small catchment on the loess plateau in China[J]. Journal of Arid Environments, 2001,48(4):537-550.
[12] Yao L, Gong Y, Ye C, et al. Soil denitrification rates are more sensitive to hydrological changes than restoration approaches in a unique riparian zone[J]. Functional Ecology, 2022,36(8):2056-2068.
[13] Ye C, Butler O M, Chen C R, et al. Shifts in characteristics of the plant-soil system associated with flooding and revegetation in the riparian zone of Three Gorges Reservoir, China[J]. Geoderma:An International Journal of Soil Science, 2020,361.
[14] Wang H L, Shu D T, Liu D, et al. Passive and active ecological restoration strategies for abandoned farmland leads to shifts in potential soil nitrogen loss by denitrification and soil denitrifying microbes[J]. Land Degradation and Development, 2020,31(9):1086-1098.
[15] Shiau Y J, Dham V, Tian G L, et al. Factors influencing removal of sewage nitrogen through denitrification in mangrove soils[J]. Wetlands:The Journal of the Society of Wetland Scientists, 2016, 36(4):621-630.
[16] Liu W Z, Xiong Z Q, Liu H, et al. Catchment agriculture and local environment affecting the soil denitrification potential and nitrous oxide production of riparian zones in the Han River Basin, China[J]. Agriculture Ecosystems&Environment, 2016,216:147-154.
[17] Wang X, Bai J, Xie T, et al. Effects of biological nitrification inhibitors on nitrogen use efficiency and greenhouse gas emissions in agricultural soils:A review[J]. Ecotoxicology and Environmental Safety, 2021,220:112338.
[18] Seitzinger S, Harrison J A, Bohlke J K, et al. Denitrification across landscapes and waterscapes:A synthesis[J]. Ecological Applications, 2006,16(6):2064-2090.
[19] Wang S, Wang W, Zhao S, et al. Anammox and denitrification separately dominate microbial N-loss in water saturated and unsaturated soils horizons of riparian zones[J]. Water Research, 2019,162:139-150.
[20] Helms J R, Mao J, Schmidt-Rohr K, et al. Photochemical flocculation of terrestrial dissolved organic matter and iron[J]. Geochimica Et Cosmochimica Acta, 2013,121:398-413.
[21] Huang S F, Chen M, Diao Y M, et al. Dissolved organic matter acting as a microbial photosensitizer drives photoelectrotrophic denitrification[J]. Environmental Science&Technology, 2022, 56(7):10.
[22] Yao L, Gong Y, Ye C, et al. Soil denitrification rates are more sensitive to hydrological changes than restoration approaches in a unique riparian zone[J]. Functional Ecology, 2022,36(8):2056-2068.
[23] Ye C, Butler O M, Chen C R, et al. Shifts in characteristics of the plant-soil system associated with flooding and revegetation in the riparian zone of Three Gorges Reservoir, China[J]. Geoderma:An International Journal of Soil Science, 2020,361.
[24] 高洁,江韬,李璐璐,等.三峡库区消落带土壤中溶解性有机质(DOM)吸收及荧光光谱特征[J].环境科学, 2015,36(1):151-162. Gao J, Jiang T, Li L L, et al. Ultraviolet-visible (UV-Vis) and fluorescence spectral characteristics of dissolved organic matter (DOM) in soils of water-level fluctuation zones of the Three Gorges Reservoir Region[J]. Environmental Science, 2015,36(1):151-162.
[25] Siemens J, Haas M, Kaupenjohann M. Dissolved organic matter induced denitrification in subsoils and aquifers?[J]. Geoderma, 2003,113(3/4):253-271.
[26] 张曾宇,李哲,方芳,等.三峡支流澎溪河消落带落干初期N₂O释放与反硝化特征初探[J].湖泊科学, 2014,26(6):8. Zhang Z Y, Li Z, Fang F, et al. Preliminary study of N₂O emission and denitrification at early exposure period in PengXi River drawdown area, Three Gorges Reservoir[J]. Journal of Lake Sciences, 2014, 26(6):8.
[27] 熊梓茜.汉江流域河-库岸带湿地植被和土壤反硝化特征[D].武汉:中国科学院大学(中国科学院武汉植物园), 2019. Xiong Z Q. Vegetation characteristics and soil denitrification of reservoir shorelines and riparian wetlands in the Han River, China[D]. Wuhan:University of Chinese Academy of Sciences (Wuhan Botanical Garden, Chinese Academy of Sciences), 2019.
[28] Yu G H, Wu M J, Wei G R, et al. Binding of organic ligands with Al (III) in dissolved organic matter from soil:Implications for soil organic carbon storage[J]. Environmental Science&Technology, 2012,46(11):6102-6109.
[29] Liu J, Liang J, Bravo A G, et al. Anaerobic and aerobic biodegradation of soil-extracted dissolved organic matter from the water-level-fluctuation zone of the three gorges reservoir region, China[J]. Science of The Total Environment, 2021,764:142857.
[30] Walters W, Hyde E R, Berg-Lyons D, et al. Improved bacterial 16S rRNA Gene (V4and V4-5) and fungal internal transcribed spacer marker gene primers for microbial community surveys[J]. mSystems, 2016,1(1):e00009-15.
[31] Shen Y F, Cheng R M, Xiao W F, et al. Temporal dynamics of soil nutrients in the riparian zone:Effects of water fluctuations after construction of the Three Gorges Dam[J]. Ecological Indicators, 2022, 139:108865.
[32] Six J, Bossuyt H, Degryze S, et al. A history of research on the link between (micro) aggregates, soil biota, and soil organic matter dynamics[J]. Soil&Tillage Research, 2004,79(1):7-31.
[33] 简尊吉,裴顺祥,郭泉水,等.三峡水库峡谷地貌区消落带土壤氮磷钾、有机质含量和pH值的时空动态[J].应用生态学报, 2017,28(9):2778-2786. Jian Z J, Pei S X, Guo Q S, et al. Temporal and spatial dynamics of soil nitrogen, phosphorus, potassium, organic matter and pH in the water level fluctuation zone of canyon landform area of the Three Gorges Reservoir, China[J]. Chinese Journal of Applied Ecology, 2017,28(9):2778-2786.
[34] 郭泉水,康义,赵玉娟,等.三峡库区消落带土壤氮磷钾、pH值和有机质变化[J].林业科学, 2012,48(3):7-10. Guo Q S, Kang Y, Zhao Y J, et al. Changes in the contents of N, P, K, pH and organic matter of the soil which experienced the hydro-fluctuation in the Three Gorges Reservoir[J]. Scientia Silvae Sinicae, 2012,48(3):7-10.
[35] Ye C, Chen C R, Butler O M, et al. Spatial and temporal dynamics of nutrients in riparian soils after nine years of operation of the Three Gorges Reservoir, China[J]. The Science of the Total Environment, 2019,664:841-850.
[36] Liu W Z, Yao L, Jiang X L, et al. Sediment denitrification in Yangtze lakes is mainly influenced by environmental conditions but not biological communities[J]. Science of The Total Environment, 2018, 616-617:978-987.
[37] Scaroni A E, Lindau C W, Nyman J A. Spatial variability of sediment denitrification across the Atchafalaya River basin, Louisiana, USA[J]. Wetlands, 2010,30:949-955.
[38] Ma L, Xiong Z Q, Yao L G, et al. Soil properties alter plant and microbial communities to modulate denitrification rates in subtropical riparian wetlands[J]. Land Degradation&Development, 2020,31(14):1792-1802.
[39] Yan Z S, Jiang H L, Cai H Y, et al. Complex interactions between the macrophyte acorus calamus and microbial fuel cells during pyrene and benzo[a]Pyrene Degradation in Sediments[J]. Scientific Reports, 2015,5:10709.
[40] Peng S C, Zhang L L, Zhang D J, et al. Denitrification synergized with ANAMMOX for the anaerobic degradation of benzene:performance and microbial community structure[J]. Applied Microbiology and Biotechnology, 2017,101(10):4315-4325.
[41] Jones C M, Allana W, Throbäck Ingela N, et al. Phenotypic and genotypic heterogeneity among closely related soil-borne N₂ and N₂O-producing Bacillus isolates harboring the nosZ gene[J]. Fems Microbiology Ecology, 2011,76(3):541-552.
[42] 王春明.三峡库区消落带土壤N₂O排放及微生物群落结构研究[D].重庆:重庆大学, 2017. Wang C M. N₂O emissions and microbial community structure of WLFZ in the Three Gorges Reservoir Area[D]. Chongqing:Chongqing University, 2017.
[43] Nuccio E E, Hodge A, Pett-Ridge J, et al. An arbuscular mycorrhizal fungus significantly modifies the soil bacterial community and nitrogen cycling during litter decomposition[J]. Environmental Microbiology, 2013,15(6):1870-1881.
[44] Wang S, Hou W J, Jiang H C, et al. Microbial diversity accumulates in a downstream direction in the Three Gorges Reservoir[J]. Journal of Environmental Sciences, 2021,101(3):156-167.