铜绿假单胞菌螯铁蛋白催化降解石油烃的机理

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摘要探讨了铜绿假单胞菌Pseudomonas aeruginosa NY3分泌的螯铁蛋白(Pyochelin,PCH)与铁形成复合物(Ferripyochelin,FerriPCH)催化降解石油烃的作用机理.结果表明,菌株NY3分泌的2种PCH为手性对映体.当纯化后的PCH与Fe³⁺鳌合时,可降解石油烃中的烷烃和多环芳烃等污染物.当PCH与Fe³⁺的比例为2:1~1:1时,对十六烷,蒽和菲有一定的降解效果,且生物利用度低的芳香烃降解效率显著高于烷烃.研究发现,Fe³⁺与PCH鳌合会产生•OH和•O₂^-自由基,这些具有强氧化性能的自由基可将石油烃逐步降解为二氧化碳和水.

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	黄芷若
	郭镝妮
	赵涛
	聂红云
	聂麦茜
	王磊
	赵碧洁

关键词 ：螯铁蛋白, FerriPCH, 催化降解, 石油烃, 自由基

Abstract：The catalytic degradation mechanism of petroleum hydrocarbons by Ferripyochelin (FerriPCH), formed by the combination of pyochelin (PCH) from the extracellular fluid of P. aeruginosa NY3 and Ferri, was investigated. The results showed that two chiral PCHs were produced by strain NY3. The purified PCH could degrade both alkanes and polycyclic aromatic compounds of petroleum hydrocarbon after combining with Ferri. A certain ratio of hexadecane, anthracene, as well as phenanthrene could be degraded by FerriPCH which formed by PCH and Ferri in the ratio from 2:1 to 1:1. In addition, degradation efficiency of aromatic hydrocarbons with low bio-availability was significantly higher than that of alkanes. Further studies showed that strongly oxidizing free radicals of •OH and •O₂^- produced after the binding of Ferri and PCH degraded petroleum hydrocarbons into carbon dioxide and water step by step.

Key words： pyochelin FerriPCH catalytic degradation petroleum hydrocarbons free radicals

收稿日期: 2024-09-11

PACS:

X172

基金资助:陕西省教育厅重点实验室项目(23JS040);陕西省秦岭生态安全重点实验室开放基金(QLES202501);陕西省科技创新团队计划(2024RS-CXTD-51)

作者简介: 黄芷若(2000-),女,湖北宜昌人,西安建筑科技大学硕士研究生,主要研究方向为难降解有机污染物的微生物处理技术.2391964534@qq.com.

引用本文:

黄芷若, 郭镝妮, 赵涛, 聂红云, 聂麦茜, 王磊, 赵碧洁. 铜绿假单胞菌螯铁蛋白催化降解石油烃的机理[J]. 中国环境科学, 2025, 45(4): 2298-2304. HUANG Zhi-ruo, GUO Di-ni, ZHAO Tao, NIE Hong-yun, NIE Mai-qian, WANG Lei, ZHAO Bi-jie. The catalytic degradation mechanism of petroleum hydrocarbons by Ferripyochelin produced from the extracellular fluid of Pseudomonas aeruginosa NY3. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(4): 2298-2304.

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