1. College of Resources Environment and Tourism, Capital Normal University, Beijing 100048, China;
2. Urban Environmental Processes and Digital Modeling Laboratory, Beijing 100048, China
Water reclamation and reuse have been actively promoted in Beijing, but the potential influences of reclaimed water on the microbial community structures are still poorly understood. Therefore, bacterial community structures in cattail rhizosphere between the samples of reclaimed water outfall and far from the reclaimed water outfall in the Mayu Wetland of Yongding River, Beijing were compared. Terminal restriction fragment length polymorphism (T-RFLP) was conducted to quantitatively detect the changes of bacterial community structures. Several statistical methods including one-way analysis of variance (ANOVA), spearman's correlation analysis and canonical correspondence analysis (CCA) were united to find out which were the key environmental factors to drive the bacterial community structure shifts. The result showed that microbial richness, evenness and diversity decreased with the increase of the reclaimed water interference intensity. The diversity of Gammaproteobacteria, Deltaproteobacteria, Chloroflexi, Epsilonproteobacteria and Actinobacteria were decreased significantly near the reclaimed water outfall. Spearman's correlation analysis indicated that pH, DO, TDS, ORP, Sal and NH4+-N play an important role in the diversity spatial variation of plant rhizosphere microbial community. CCA indicated that TN, TOC, and Cr、Ni、Cu were significantly correlated with microbial communities structures of the upstream of reclaimed water outfall. Plant rhizosphere bacterial communities near the outfall were significantly different due to the reclaimed water quality difference. Group IIa and IIb were mainly affected by pH and T、ORP、NH4+-N, respectively. While the bacterial communities in the downstream were also significantly correlated with TOC and some heavy metals due to water internal impurities and human activity influence.
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