Community structure and influencing factors of bacterioplankton during low water periods in Danjiangkou Reservoir
CHEN Zhao-jin1,2, DING Chuan-yu1,2, ZHU Jing-ya1,2, LI Bing2,3, HUANG Jin2,3, DU Zong-ming2,3, WANG Ya2,3, LI Yu-ying1,2
1. Key Laboratory of Ecological Security for Water Source Region of Mid-line Project of South-to-North Diversion Project of Henan Province, College of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China;
2. Henan Collaborative Innovation Center of Water Security for Water Source Region of Mid-line Project of South to North Diversion Project, Nanyang 473061, China;
3. Emergency Centre for Environmental Monitoring of Mid-line Project of South to North Water Division, Xichuan 474475, China
The Danjiangkou reservoir is the main source of water for the "middle-route" of the South to North Water Diversion Project in China. Its water quality directly affects the safety of drinking water for residents along this route. In this study, 15surface-water samples were collected from the reservoir at low-water period (January) in 2016, and 12 essential environmental factors were investigated. The results showed that the water quality in the reservoir was strongly influenced by total nitrogen (TN) and the permanganate index, with the water quality being ranked at IV or Ⅱ for all locations. The water in this reservoir was in the mesotrophic state. The high-throughput sequencing approach was adopted to analyze the distribution characteristics of the community structure and diversity of bacterioplankton and their relationship to environmental factors. Phylogenetic analysis based on 16S rDNA sequences showed that bacterioplankton could be divided into 22 major phylogenetic groups. The dominant phylogenetic groups included Actinobacteria, Proteobacteria, Cyanobacteria, Bacteroidetes, Verrucomicrobia, Firmicutes, and Acidobacteria. At the genus level, the dominant phylogenetic groups included CL500-29 marine group, hgcI clade, Fluviicola, Sediminibacterium, Flavobacterium, LD28 freshwater group, Limnohabitans, Synechococcus, Bacillus, and Cyanobacteria norank. Diversity of the bacterioplankton community in the Danjiangkou reservoir can be ranked in the following order:Heijizui > Taizishan > Kuxin> Songgang > Qushou. The results of canonical correspondence analysis (CCA) for the relationship between the 22 phyla of bacterioplankton and 12environmental factors showed that the T, pH, NH4+-N, and SD were the main environmental factors affecting the distribution characteristics of the bacterioplankton community.
陈兆进, 丁传雨, 朱静亚, 李冰, 黄进, 杜宗明, 王亚, 李玉英. 丹江口水库枯水期浮游细菌群落组成及影响因素研究[J]. 中国环境科学, 2017, 37(1): 336-344.
CHEN Zhao-jin, DING Chuan-yu, ZHU Jing-ya, LI Bing, HUANG Jin, DU Zong-ming, WANG Ya, LI Yu-ying. Community structure and influencing factors of bacterioplankton during low water periods in Danjiangkou Reservoir. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(1): 336-344.
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