Abstract：The community H and single-strain DYC-1 was isolated by timing quantitative culture and domestication method of adding the concentration of PAHs gradatim from Tianjin coastal wetland petroleum contaminated soil under the low temperature condition. The community H and single-strain DYC-1used pyrene as solo carbon source for growth. The bacteria were identified as Rhodococcus sp. based on the BLAST sequence analysis of 16s rDNA and its morphological and physio-biochemcial characteristics. Analysis results showed that the degrading ability of single-strain DYC-1 was similar to community H under the low temperature condition. The degradation rate of them was above 35% at high concentrations of pyrene. The single-strain DYC-1 had more salt-resisting ability and wide substrate degradation ability. The optimal degradation conditions of single-strain DYC-1 was 2% salinity, pH8, 10℃, 110r/min rotate speed and 5% capacity of bacteria. The degradation rate of pyrene was high with 20mg/L initial concentration in the optimal degradation conditions.
Hua F, Wang H. Uptake modes of octadecane by Pseudomonas, sp. DG17 and synthesis of biosurfactant[J]. Journal of Applied Microbiology, 2012,112(1):25-37.
[2]
Mackay D, Shiu W Y, Ma K C. Illustrated handbook of physical-chemical properties and environmental fate for organic chemicals. Volume 5:pesticide chemicals.[M]//Illustrated handbook of physical-chemical properties and environmental fate for organic chemicals/. Lewis Publishers, 1992:551-552.
Whyte L G, Greer C W, Inniss W E. Assessment of the biodegradation potential of psychrotrophic microorganisms.[J]. Canadian Journal of Microbiology, 1996,42(2):99-106.
[6]
Deppe U, Richnow H H, Michaelis W, et al. Degradation of crude oil by an arctic microbial consortium[J]. Extremophiles, 2005, 9(6):461-70.
Patel J G, Kumar J I N, Kumar R N, et al. Enhancement of pyrene degradation efficacy of Synechocystis sp. by construction of an artificial microalgal-bacterial consortium[J]. Cogent Chemistry, 2015,1(1).
