1. 北京工业大学, 北京市水质科学与水环境恢复工程重点实验室, 北京 100124;
2. Dalhousie University, Department of Civil and Resource Engineering, Halifax Canada
Way of nitrogen and phosphorus removal and microbial community characteristics for SCSC-S/Fe
FAN Jun-hui1, HAO Rui-xia1, LIU Lei2, ZHU Xiao-xia1, WANG Wei-dong1, WAN Jing-jing1
1. Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100124, China;
2. Department of Civil and Resource Engineering, Dalhousie University, Halifax, Canada
In order to improve the quality of reclaimed water, a coupling process with solid carbon source of corncob cellulose and sulfur/sponge iron composite fillers was integrated for the further deep removal of nitrogen and phosphorus for municipal wastewater treatment plant (WWTP) effluent that usually contains little organic carbon for heterotrophic denitrifying bacteria to utilize. Based on the bacteria 16S rRNA gene clone library analysis by adopting the high-throughput sequencing technologies, both the effect and mechanism of the system (abbreviated as SCSC-S/Fe) on nitrogen (N) and phosphorus (P) simultaneous removal from simulated WWTP effluent were investigated. The results indicated that the TN removal efficiency of the system increased gradually with the increase of temperature, but the removal rate of TP did not raised obviously. Under the condition of 30℃temperature and 9h HRT, the average removal rates of NO3--N, TN and TP were 99.86%, 92.70% and 89.15%, respectively. There were two main classes of bacteria including cellulose degradation and denitrification bacteria with the total bacteria of 41.37% and 54.02% separately in the solid carbon source of corncob cellulose unit. And there were simultaneous existence of heterotrophic, hydrogen and sulfur autotrophic denitrifying bacteria accounted for 91.53% of the total number of bacteria in the sulfur/sponge iron composite fillers unit. X-ray diffraction (XRD) analysis showed that PO43- was removed mainly by the form of FePO4, Fe3(PO4)2·χH2O and Fe3 (PO4)3(OH)2. In the system, the nitrogen was removed mainly by heterotrophic denitrification with auxiliary hydrogen and sulfur autotrophic denitrification, and the phosphorus was removed by main chemical reaction and a little biological uptake of phosphate. SCSC-S/Fe system represents a promising method for simultaneous and improved N and P removal from WWTP effluent.
范军辉, 郝瑞霞, LIU Lei, 朱晓霞, 王卫东, 万京京. SCSC-S/Fe复合系统脱氮除磷途径及微生物群落特性[J]. 中国环境科学, 2017, 37(4): 1358-1365.
FAN Jun-hui, HAO Rui-xia, LIU Lei, ZHU Xiao-xia, WANG Wei-dong, WAN Jing-jing. Way of nitrogen and phosphorus removal and microbial community characteristics for SCSC-S/Fe. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(4): 1358-1365.
Ovez B. Batch biological denitrification using Arundo donax, Glycyrrhiza glabra, and Gracilaria verrucosa as carbon source[J]. Process Biochemistry, 2006,41(6):1289-1295.
[2]
Ovez B, Ozgen S, Yuksel M. Biological denitrification in drinking water using Glycyrrhiza glabra and Arunda donax as the carbon source[J]. Process Biochemistry, 2006,41(7):1539-1544.
[3]
Volokita M, Belkin S, Abeliovich A, et al. Biological denitrification of drinking water using newspaper[J]. Water Research, 1996,30(4):965-971.
Till B A, Weathers L J, Alvarez P J J. Fe(0)-Supported Autotrophic Denitrification[J]. Environmental Science & Technology, 1998,32(5):634-639.
[10]
Shin K H, Cha D K. Microbial reduction of nitrate in the presence of nanoscale zero-valent iron[J]. Chemosphere, 2008,72(2):257- 262.
[11]
Kielemoes J, And P D B, Verstraete W. Influence of Denitrification on the Corrosion of Iron and Stainless Steel Powder[J]. Environmental Science & Technology, 2000,34(4):663-671.
[12]
Batchelor B, Lawrence A W. Autotrophic Denitrification Using Elemental Sulfur[J]. Journal, 1978,50(8):1986-2001.
[13]
闵 乐.催化铁内电解法生产性试验和使用镀铜电极的研究[D]. 上海:同济大学, 2006.
[14]
Chitrakar R, Makita Y, Hirotsu T, et al. Selective Uptake by Akaganeite (β-FeOOH) of Phosphite from Hypophosphite and Phosphite Solutions[J]. Chemistry Letters, 2012,41(12):1694- 1696.
Naumoff D G, Dedysh S N. Lateral gene transfer between the Bacteroidetes, and Acidobacteria:The case of α-l-rhamnosidases[J]. Febs Letters, 2012,586(21):3843-3851.
[17]
Feng C J, Zhang Z J, Wang S M, et al. Characterization of microbial community structure in a hybrid biofilm-activated sludge reactor for simultaneous nitrogen and phosphorus removal[J]. Journal of Environmental Biology, 2013,34(2):489-499.
[18]
Shen Z, Zhou Y, Hu J, et al. Denitrification performance and microbial diversity in a packed-bed bioreactor using biodegradable polymer as carbon source and biofilm support[J]. Journal of Hazardous Materials, 2013,250-251(8):431-438.
[19]
Knowles R. Denitrification[J]. Microbiological Reviews, 1982, 46(1):43-70.
Wang H Y, Zhou Y X, Yuan Q, et al. Bacteria morphology and diversity of the combined autotrophic nitritation and sulfur- carbon three-dimensional-electrode denitrification process[J]. Journal of Environmental Science and Health, Part A-Toxic/Hazardous Substances & Environmental Engineering. 2014,49(1):39-51.
[22]
Jean M R, Gonzalez-Rizzo S, Gauffre-Autelin P, et al. Two new Beggiatoa species inhabiting marine mangrove sediments in the Caribbean.[J]. Plos One, 2015,10(2):1-16.
[23]
Hao R X, Li S M, Li J B, et al. Denitrification of simulated municipal wastewater treatment plant effluent using a three- dimensional biofilm-electrode reactor:Operating performance and bacterial community[J]. BioresourceTechnology, 2013, 143(6):178-186.
[24]
Shinoda Y, Sakai Y, Uenishi H, et al. Aerobic and anaerobic toluene degradation by a newly isolated denitrifying bacterium, Thauerasp strain DNT-1[J]. Applied And Environmental Microbiology, 2004,70(3):1385-1392.
[25]
Bucher B, Poupard J, Parra J P, et al. Borrelia burgdorferi and tropical spastic paraparesis[J]. Lancet, 1986,336(8723):1135- 1135.
[26]
Cardinali-Rezende J, Araújo J C, Almeida P G S, et al. Organic loading rate and food-to-microorganism ratio shape prokaryotic diversity in a demo-scale up-flow anaerobic sludge blanket reactor treating domestic wastewater[J]. Antonie Van Leeuwenhoek, 2013,104(6):993-1003.
[27]
Shen R, Liu Z, He Y, et al. Microbial electrolysis cell to treat hydrothermal liquefied wastewater from cornstalk and recover hydrogen:Degradation of organic compounds and characterization of microbial community[J]. International Journal of Hydrogen Energy, 2016,41(7):4132-4142.
[28]
Shao M F, Zhang T, Fang H P. Sulfur-driven autotrophic denitrification:diversity, biochemistry, and engineering applications[J]. Applied Microbiology & Biotechnology, 2010, 88(5):1027-1042.
[29]
Wang H Y, Zhou Y X, Yuan Q, et al. Bacteria morphology and diversity of the combined autotrophic nitritation and sulfur- carbon three-dimensional-electrode denitrification process[J]. Journal of Environmental Science and Health Part A-Toxic/hazardous Substances & Environmental Engineering, 2014,49(1):39-51.
[30]
PengL, Wei X, Zuo J, et al. Hydrogenotrophic denitrification for tertiary nitrogen removal from municipal wastewater using membrane diffusion packed-bed bioreactor[J]. Bioresource Technology, 2013,144(5):452-459.
[31]
Vasiliadou I, Siozios S, Papadas I, et al. Kinetics of pure cultures of hydrogen-oxidizing denitrifying bacteria and modeling of the interactions among them in mixed cultures[J]. Biotechnology & Bioengineering, 2006,95(3):513-525.
Hao R, Meng C, Li J. An integrated process of three-dimensional biofilm-electrode with sulfur autotrophic denitrification (3DBER-SAD) for wastewater reclamation.[J]. Applied Microbiology and Biotechnology, 2016,100(16):7339-7348.
Ding X, Song X, Boily J F. Identification of Fluoride and Phosphate Binding Sites at FeOOH Surfaces[J]. Journal of Physical Chemistry C, 2012,116(41):21939-21947.
