1. Key laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China;
2. College of Environment, Nanjing Tech University, Nanjing 210009, China
In the present study, the changes of plant height, root length, plant fresh weight, amylase activity, AKP activity, GSH content and MDA content of rice (Oryza sativa L.) were investigated under the exposure to a series of concentrations of MC-LR (0.1μg/L, 1.0μg/L and 10.0μg/L) and Microcystis aeruginosa crude extracts (0.002lysate, 0.02lysate and 0.2lysate) for 21d. Results showed that the plant height, root length and amylase activities of rice were decreased under the exposure of pure MC-LR. Root length was a sensitive indicator in rice for its quick response to MC-LR exposure in 0.1μg/LMC-LR treatment group. However, Microcystis aeruginosa crude extracts with high concentration of MC-LR had positive effects on growth and development of rice except for root length. GSH content of rice was significantly induced in 0.1μg/L MC-LR group, but no significant changes of AKP activity was observed in all pure MC-LR treatment groups. In contrast, GSH content, MDA content and AKP activity could be significant inhibited in rice exposed to Microcystis aeruginosa crude extracts. These results suggested that the toxicity mechanism of MC-LR to rice might be different from Microcystis aeruginosa crude extracts and other toxic components existed in Microcystis aeruginosa crude extracts may exert great influence on rice.
Wiegand C. Pflugmacher S. Ecotoxicological effects of selected cyanobacterial secondary metabolites a short review[J]. Toxicology and Applied Pharmacology, 2005,203(3):201-218.
[2]
Spoof L, Neffling M R, Meriluoto J. Fast separation of microcystins and nodularins on narrow-bore reversed-phase columns coupled to a conventional HPLC system[J]. Toxicon, 2010,55(5):954-964.
Lahti K, Rapala J, Frdig M, et al. Persistence of cyanobacterial hepatotoxin, microcystin-LR in particulate material and dissolved in lake water[J]. Water Research, 1997,31(5):1005-1012.
[5]
Jones G J, Orr P T. Release and degradation of microcystin following algicide treatment of a Microcystis aeruginosa bloom in a recreational lake, as determined by HPLC and protein phosphatase inhibition assay[J]. Water Research, 1994,28(4):871-876.
Ozawa K, Yokoyama A, Ishikawa K, et al. Accumulation and depuration of microcystin produced by the cyanobacterium Microcystis in freshwater snail[J]. Limnology, 2003,4:131-138.
Chen J, Zhang D W, Xie P, et al. Simultaneous determination of microcystin contaminations in various vertebrate (fish, turtle, duck and water bird) from a large eutrophic Chinese lake, Lake Taihu, with toxic Microcystis blooms[J]. Science of the Total Environment, 2009,407(10):3317-3322.
Corbel S, Mougin C, Bouaïcha N. Cyanobacterial toxins:modes of actions, fate in aquatic and soil ecosystems, phytotoxicity and bioaccumulation in agricultural crops[J]. Chemosphere, 2014, 96:1-15.
[12]
Romero C S, Contardo V, Block T, et al. Accumulation of microcystin congeners in different aquatic plants and crops-A case study from lake Amatitlán, Guatemala[J]. Ecotoxicology and Environmental Safety, 2014,102(1):121-128.
[13]
Chen J, Han F X, Wang F, et al. Accumulation and phytotoxicity of microcystin-LR in rice(Oryza sativa)[J]. Ecotoxicology and Environmental Safety, 2012,76(2):193-199.
Chen J, Song L, Dai J, et al. Effects of microcystins on the growth and the activity of superoxide dismutase and peroxidase of rape (Brassica napus L.) and rice (Oryza sativa L.)[J]. Toxicon, 2004,43(4):393-400.
Craig M, Luu H A, McCready T L, et al. Molecular mechanisms underlying the interaction of motuporin and microcystins with type-1and type-2A protein phosphatases[J]. Biochemistry and cell biology, 1996,74(4):569-578.
EL Khalloufi F, EL Ghazali I, Saqrane S, et al. Phytotoxic effects of a natural bloom extract containing microcystins on Lycopersicon esculentum[J]. Ecotoxicology and Environmental Safety, 2012,79:199-205.
Pflugmacher S. Promotion of oxidative stress in the aquatic macrophyte Ceratophyllum demersum during biotransformation of the cyanobacterial toxin microcystin-LR[J]. Aquatic Toxicology, 2004,70(3):169-178.
