Bio-reduction of perchlorate with low concentration in water by sulfur packed reactor and microbial community spacial distribution analysis
LIU Yong-de1, WANG Yi-yi1, WAN Dong-jin1, XIAO Shu-hu2
1. School of Chemical Engineering and Environment, Henan University of Technology, Zhengzhou 450001, China;
2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
Removal of perchlorate with low concentration[(468.74±6.80)μg/L] in water was investigated by an up-flow sulfur autotrophic reduction reactor. And bacterial community spatial distribution was analyzed by High-throughput sequencing method. The reactor could be operated at a hydraulic retention time (HRT) ranging in 4.00~0.75h with a remarkable removal efficiency greater than 97%. 1/2-order kinetics model fit the experimental data well; and 1/2K1/2v was 39.59[μg1/2/(L1/2·h)]. When HRT shortened from 4.00h to 0.75h, the generated SO42- decreased from 173.37 to 90.07mg/L. Sulfur (S) disproportionation was accompanied with perchlorate reduction; the proportion of SO42- generated by S-disproportionation was in range of 90.75%~93.91%. Meanwhile, S-disproportionation was the main reason for excess consumption of alkalinity, thus leading to pH decreases in effluent. The sequencing results showed that the α-biodiversity was decreased along the height of reactor. The Proteobacteria and Chlorobi was observed as the major bacteria, and the Chlorobaculum was the dominant bacteria associated with S-disproportionation.
刘永德, 王依依, 万东锦, 肖书虎. 硫自养微生物降解水中低浓度高氯酸盐的研究——反应器效能及微生物种群空间分布[J]. 中国环境科学, 2017, 37(8): 3142-3150.
LIU Yong-de, WANG Yi-yi, WAN Dong-jin, XIAO Shu-hu. Bio-reduction of perchlorate with low concentration in water by sulfur packed reactor and microbial community spacial distribution analysis. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(8): 3142-3150.
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