High-throughput sequencing sevealed soil fungal communities under three terrace agrotypes on the loess plateau
XIAO Li, HUANG Yi-mei, ZHAO Jun-feng, ZHOU Jun-ying, GUO Ze-hui, LIU Yang
Key Laboratory of Plant Nutrition and The Agri-environment in Northwest China, Ministry of Agriculture, College of Natural Resource and Environment, Northwest A & F University, Yangling 712100, China
Deep understanding of soil fungal communities and their relationships with soil physicochemical properties is of great importance for assessing soil fertility and environment effect, especially for terraces on the Loess Plateau. Soil fungal community composition and diversity was analyzed using the Hiseq high-throughput sequencing technology. Three typical crops soil (Corn field (CF), Apple field (AF) and Intercropping (apple and potatoes, IC) with similar terrace construction were chosen as experimental sites on Loess Plateau. The results showed among three sites, the number of the identified OTU ranged from 688 to 862 including 13 phylum, 87 class, 164 order, and the diversity index of the Chao1, ACE, Shannon and Simpson were all high in AF and IC than in CF. Three most abundant fungal taxa were Ascomycota, Basidiomycota and Zygomycota, with a highest relative abundance of Ascomycota found in IC, which mainly affected by the content of soil TN and AK. Composition of soil fungi was different for application of organic manure under terrace crop systems shifts soil fungi community composition by increased soil nutrient, and thus has potential to promote soil fertility in terrace agricultural ecosystem.
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