Denitrification performance of the bacterium Diaphorobacter polyhydroxybutyrativorans SL-205
ZHANG Shu-song1,2, FAN Yue-ting1,2, SUN Xing-bin1, QIU Tian-lei2, GAO Min2, WANG Xu-ming2,3
1. College of Life Science, Northeast Forestry University, Harbin 150040, China;
2. Beijing Agro-Biotechnology Research Center, Beijng Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
3. Key Laboratory of Urban Agriculture(North), Ministry of Agriculture, Beijing 100097, China
In order to enhance nitrogen removal from nitrate-contaminated water, a novel bacterial strain, Diaphorobacter polyhydroxybutyrativorans SL-205T, was investigated to use different carbon sources for aNOxic denitrification. Additionally, the aerobic denitrification performance for strain SL-205T was studied using poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) as the sole carbon source. The results showed that the removal efficiencies of nitrate reached up to over 99% by strain SL-205T using sodium acetate, sodium succinate and PHBV as carbon sources under the aNOxic conditions. The optimum addition dosage of PHBV was 2.0g/L with 315mg/L of the initial nitrate nitrogen. Strain SL-205T had the capability to fully remove as much as 97.4% of nitrate nitrogen, and the average removal rate for nitrate nitrogen was 8.69mg/(L·h) after 36h under the aerobic conditions. Meanwhile, both nitrite and nitrous oxide were not accumulated. The results above-mentioned would lay an experimental foundation for nitrogen removal from wastewater by strain SL-205T.
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