Comparison to the characteristics and archaea communites under different temperature stages of thermophilic, mesophilic and psychrophilic of fixed bed anaerobic reactor
ZHAO Hong-yan1, LI Xue1, LI Nan1, LIU Hong-dou1, PIAO Ren-zhe1, XU Guang-bo1, LI Yan-ru1, WANG Wei-dong2, CUI Zong-jun3
1. Agricultural College, Yanbian University, Yanji 133002, China; 2. Life Sciences and Biotechnology, Heilongjiang August First Land Reclamation University, Daqing 163000, China; 3. Center of Biomass Engineering, Agricultural University, Beijing 100091, China
Abstract：In order to investigate the change of characteristics and methanogenic microorganisms under three temperature stages. Comparison to the dynamic of methane character and archaea communites under three temperature stages of thermophilic (50℃), mesophilic(35℃) and psychrophilic(4℃). The result showed that the order of absolute biogas production from high to low was thermophilic (50℃), mesophilic(35℃) and psychrophilic(4℃). The order of biogas production mesophilic, psychrophilic and thermophilic was 2.84, 2.5 and 1.8L/OLR, respectively. The order of methane content psychrophilic, mesophilic and thermophilic was 74.5, 63.5 and 57.3%, respectively. The volatile organic acids content was a big influence under different temperature stage. Clone library analysis that the diversity of microbiology was significant difference within different temperature stage. Quantitative PCR analysis that the methanosaetaceae was the dominant methanogens within 50℃ and 35℃reactors, the methanomicrobiales was the dominant methanogens in the adhering sludge at 4℃. From energy consumption an economic benefit analysis that the biogas fermentation was more suitable under low temperature, and the main metabolic pathways was hydrogenotrophic methanogenesis.
赵洪颜, 李雪, 李楠, 刘虹豆, 朴仁哲, 许广波, 李艳茹, 王伟东, 崔宗均. 温度对FBAR反应器的运行特性及古菌微生物群落影响[J]. 中国环境科学, 2018, 38(1): 169-178.
ZHAO Hong-yan, LI Xue, LI Nan, LIU Hong-dou, PIAO Ren-zhe, XU Guang-bo, LI Yan-ru, WANG Wei-dong, CUI Zong-jun. Comparison to the characteristics and archaea communites under different temperature stages of thermophilic, mesophilic and psychrophilic of fixed bed anaerobic reactor. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(1): 169-178.
El-Mashad H M, Zeeman G, van Loon W K, et al. Effect of temperature and temperature fluctuation on thermophilic anaerobic digestion of cattle manure[J]. Bioresource Technology, 2004,95:191-201.
[2]
Akuzawa M, Hori T, Haruta S, et al. Distinctive responses of metabolically active microbiota to acidification in a thermophilic anaerobic digester[J]. Microbial Ecology, 2011,61:595-605.
[3]
Lettinga G, Rebac S, Zeeman G. Challenge of psychrophilic anaerobic wastewater treatment, TRENDS in Biotechnology[J]. 2001,19:363-370.
Hori T, Haruta S, Ueno Y, et al. Dynamic transition of a methanogenic population in response to the concentration of volatile fatty acids in a thermophilic anaerobic digester[J]. Appl. Environ. Microbiol., 2006,72:1623-1630.
[6]
O'Reilly J, Lee C, Collins G, et al. Quantitative and qualitative analysis of methanogenic communities in mesophilically and psychrophilically cultivated anaerobic granular biofilims[J]. Water Res., 2009,43:3365-3374.
[7]
Zhang D, Li J, Guo P, et al. Dynamic transition of microbial communities in response to acidification in fixed-bed anaerobic baffled reactors (FABR) of two different flow directions[J]. Bioresource Technology, 2011,102:4703-4711.
[8]
Bialek K, Kim J, Lee C, et al. Quantitative and qualitative analyses of methanogenic community development in high-rate anaerobic bioreactors[J]. Water Research, 2011,45:1298-1308.
[9]
贺延龄.废水的厌氧生物处理[M]. 北京:中国轻工业出版社, 1998.
[10]
Wang X, Haruta S, Wang P, et al. Diversity of a stable enrichment culture which is useful for silage inoculant and its succession in alfalfa silage[J]. FEMS Microbiology Ecology, 2006,57:106-115.
[11]
Lueders T, Friedrich M W. Effects of Amendment with Ferrihydrite and Gypsum on the Structure and Activity of Methanogenic Populations in Rice Field Soil[J]. Applied and Environmental Microbiology, 2002,68:2484-2494.
[12]
Gao L J, Yang H Y, Wang X F, et al. Rice straw fermentation using lactic acid bacteria[J]. Bioresource Technology, 2008,99:2742-2748.
[13]
Yu Y, Lee C, Kim J, et al. Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction[J]. Biotechnol. Bioeng., 2005,89:670-679.
[14]
Enright A M, Collins G, O'Flaherty V. Temporal microbial diversity changes in solvent-degrading anaerobic granular sludge from low-temperature (15degrees C) wastewater treatment bioreactors[J]. Syst. Appl. Microbiol., 2007,30:471-482.
McKeown R M, Scully C, Mahony T, et al. Long-term (1,243days), low-temperature (4-15degrees C), anaerobic biotreatment of acidified wastewaters:bioprocess performance and physiological characteristics[J]. Water Res., 2009,43:1611-1620.
[17]
Kalyuzhnyl S V, Skylar M A, Kizimenko V I, et al. One-and Two-Stage Upflow Anaerobic Sludge-Bed Reactor Pretreatment of Winery Wastewater at 4-10℃[J]. Appl. Biochem. Biotechnol., 2001,90:107-124.
[18]
Zhang D D, Zhu W B, Tang C,et al. Bioreactor performance and methanogenic population dynamics in a low-temperature (5-18degrees C) anaerobic fixed-bed reactor[J]. Bioresource Technology, 2012,104:136-143.
[19]
Dhaked R K, Singh P, Singh L. Biomethanation under psychrophilic conditions[J]. Waste Management, 2010,30:2490-2496.
[20]
Xia Y, Wang Y, Fang H H, et al. Thermophilic microbial cellulose decomposition and methanogenesis pathways recharacterized by metatranscriptomic and metagenomic analysis[J]. Sci. Rep., 2014,(4):6708.
Ahring B K. Methanogenesis in thermophilic biogas reactors[J]. Antonie van Leeuwenhoek, 1995,67:91-102.
[23]
Petersen S P, Ahring B K. Acetate oxidation in a thermophilic anaerobic sewage-sludge digestor:the importance of nonaceticlastic methanogenesis from acetate[J]. FEMS Microbiology Letters, 1991,86:149-152.
[24]
Sakai S, Imachi H, Sekiguchi Y,et al. Cultivation of methanogens under low-hydrogen conditions by using the coculture method[J]. Applied and Environmental Microbiology, 2009,75:4892-4896.
[25]
McKeown R M, Hughes D, Collins G, et al. Low-temperature anaerobic digestion for wastewater treatment[J]. Current Opinion in Biotechnology, 2012,23:444-451.
[26]
O'Reilly J, Lee C, Chinalia F, et al. Microbial community dynamics associated with biomass granulation in lowtemperature (15degrees C) anaerobic wastewater treatment bioreactors[J]. Bioresour. Technol., 2010,101:6336-6344.
[27]
Dolfing J, Larter S R, Head I M. Thermodynamic constraints on methanogenic crude oil biodegradation[J]. The ISME Journal, 2007,2:442-452.
[28]
Conrad R, Wetter B. Influence of temperature on energetics of hydrogen metabolism in homoacetogenic, methanogenic, and other anaerobic bacteria[J]. Archives of Microbiology, 1990, 155:94-98.
[29]
McHugh S, Collins G, Flaherty V O. Long-term, high-rate anaerobic biological treatment of whey wastewaters at psychrophilic temperatures[J]. Bioresour. Technol., 2006,97:1669-1678.
McHugh S, Carton M, Mahony T, et al. Methanogenic population structure in a variety of anaerobic bioreactors[J]. FEMS Microbiology Letters, 2003,219:297-304.
