Advanced treatment of digested sludge using an aerobic bacterium
HE Pin-jing1,2, WANG Yue1, HU Jie1, SHAO Li-ming2, LÜ Fan1
1. Institute of Waste Treatment and Reclamation, Tongji University, Shanghai 200092, China; 2. Centre for the Technology Research and Training on Household Waste in Small Towns & Rural Area, Ministry of Housing Urban-Rural Development, Shanghai 200092, China
Abstract:In order to evaluate the feasibility of applying Bacillus licheniformis as an aerobic bacterium on advanced treatment of digested sludge, dissolved organic carbon, dissolved total nitrogen, ammonia nitrogen concentration in the liquid phase of digested sludge and the protein, ammonia nitrogen concentration and modeled CST value in the digested sludge flocs, were measured at different inoculation ratios (respectively 2.7×10-3, 2.7×10-2, 2.7×10-1, total solid ratio), while the effects of mechanical disintegration method and bacterial treatment method on secondary digestion and gas production of digested sludge were also compared. The results showed that the addition of Bacillus licheniformis required a higher inoculation ratio to significantly promote the dissolution of digested sludge into the phase and to improve the biodegradability of digested sludge. When the inoculation ratio of Bacillus licheniformis was 2.7×10-1, DOC, DN, ammonia nitrogen concentration in the liquid phase and ammonia nitrogen concentration in the digested sludge flocs reached the maximum accumulative values during the bacterial treatment, which was respectively 6.23, 2.83, 5.93 and 4.94 times of that of the control group. The maximum degradation rate of protein was also observed in the digested sludge flocs. At this inoculation ratio 2.7×10-1, the biochemical methane potential of the digested sludge was superior to that of other inoculation ratio and was 5.96 times of that of the digested sludge treated by mechanical disintegration. Therefore, this method of bacterial treatment was beneficial to further deepen the utilization of residual refractory organic matter in digested sludge and to improve its biodegradability and final stability after digestion. However, after the bacterial treatment, the modeled CST value of digested sludge was 2.69 times of that of the control group, which deteriorated the dewaterability of the digested sludge.
何品晶, 王玥, 胡洁, 邵立明, 吕凡. 应用好氧生物菌剂深度处理消化污泥[J]. 中国环境科学, 2018, 38(1): 222-233.
HE Pin-jing, WANG Yue, HU Jie, SHAO Li-ming, LÜ Fan. Advanced treatment of digested sludge using an aerobic bacterium. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(1): 222-233.
Wei Y, van Houten R T, Borger A R, et al. Comparison performances of membrane bioreactor and conventional activated sludge prcesses on sludge reduction induced by Oligochaete[J]. Environmental Science & Technology, 2003,37(14):3171-3180.
[3]
Zhang D, Chen Y, Zhao Y, et al. New Sludge Pretreatment, Method to Improve Methane Production in Waste Activated Sludge Digestion[J]. Environmental Science & Technology. 2010,44(12):4802-4808.
[4]
Appels L, Lauwers J, Degrève J, et al. Anaerobic digestion in global bio-energy production:Potential and research challenges[J]. Renewable & Sustainable Energy Reviews, 2011,15(9):4295-4301.
[5]
Astals S, Esteban-Gutiérrez M, Fernández-Arévalo T, et al. Anaerobic digestion of seven different sewage sludges:a biodegradability and modelling study[J]. Water Research, 2013, 47(16):6033-6043.
[6]
Yang G, Zhang G, Zhuan R, et al. A critical review on sludge anaerobic digestion progress from 2012 to 2015[J]. Current Organic Chemistry, 2016,20(999):1.
[7]
Fan L, Li T, Wang T, et al. Improvement of sludge digestate biodegradability by thermophilic bioaugmentation[J]. Applied Microbiology and Biotechnology, 2014,98(2):969-977.
[8]
Novak J T, Sadler M E, Murthy S N. Mechanisms of floc destruction during anaerobic and aerobic digestion and the effect on conditioning and dewatering of biosolids[J]. Water Research, 2003,37(13):3136-3144.
[9]
Batstone D J, Tait S, Starrenburg D. Estimation of hydrolysis parameters in full-scale anerobic digesters[J]. Biotechnology and Bioengineering, 2009,102(5):1513-1520.
[10]
Suhartini S, Heaven S, Banks C J. Comparison of mesophilic and thermophilic anaerobic digestion of sugar beet pulp:performance, dewaterability and foam control[J]. Bioresource Technology, 2014,152(1):202-211.
[11]
Wang T, Shao L, Li T, et al. Digestion and dewatering characteristics of waste activated sludge treated by an anaerobic biofilm system[J]. Bioresource Technology, 2014,153(2):131-136.
[12]
Zhen G, Lu X, Kato H, et al. Overview of pretreatment strategies for enhancing sewage sludge disintegration and subsequent anaerobic digestion:Current advances, full-scale application and future perspectives[J]. Renewable & Sustainable Energy Reviews, 2017,69:559-577.
Onyeche T I. Economic benefits of low pressure sludge homogenization for wastewater treatment plants[Z]. 2007.
[15]
Ayol A. Enzymatic treatment effects on dewaterability of anaerobically digested biosolids-I:performance evaluations[J]. Process Biochemistry, 2005,40(7):2427-2434.
[16]
Hasegawa S, Shiota N, Katsura K, et al. Solubilization of organic sludge by thermophilic aerobic bacteria as a pretreatment for anaerobic digestion[J]. Water Science & Technology, 2000,41(3):163-169.
[17]
Miah M S, Tada C, Yang Y, et al. Aerobic thermophilic bacteria enhance biogas production[J]. Journal of Material Cycles and Waste Management, 2005,7(1):48-54.
[18]
Shao L, Wang X, Xu H, et al. Enhanced anaerobic digestion and sludge dewaterability by alkaline pretreatment and its mechanism[J]. Journal of Environmental Sciences, 2012,24(10):1731-1738.
Kim Y, Cho J Y, Kuk J H, et al. Identification and Antimicrobial Activity of Phenylacetic Acid Produced by Bacillus licheniformis Isolated from Fermented Soybean, Chungkook-Jang[J]. Current Microbiology, 2004,48(4):312-317.
[23]
Tamehiro N, Okamotohosoya Y, Okamoto S, et al. Bacilysocin, a novel phospholipid antibiotic produced by Bacillus subtilis 168[J]. Antimicrobial Agents & Chemotherapy, 2002,46(2):315-320.
Lin Y, Lü F, Shao L, et al. Influence of bicarbonate buffer on the methanogenetic pathway during thermophilic anaerobic digestion[J]. Bioresource Technology, 2013,137C(6):245-253.
[28]
Merlo R P, Trussell R S, Hermanowicz S W, et al. A comparison of the physical, chemical, and biological properties of sludges from a complete-mix activated sludge reactor and a submerged membrane bioreactor[J]. Water Environment Research A Research Publication of the Water Environment Federation, 2007,79(3):320-328.
[29]
Zepp R G, Sheldon W M, Moran M A. Dissolved organic fluorophores in southeastern US coastal waters:correction method for eliminating Rayleigh and Raman scattering peaks in excitation-emission matrices[J]. Journal of Environmental Sciences, 2004,21(6):795-800.
[30]
Lu F, Chang C, Lee D, et al. Dissolved organic matter with multi-peak fluorophores in landfill leachate[J]. Chemosphere, 2009,74(4):575-582.
[31]
Chen W, Westerhoff P, Leenheer J A, et al. Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter[J]. Environmental Science & Technology, 2003,37(24):5701.