Effects of sludge concentration on microbial community structure in A/O process
LUO Xiao1,2, ZHENG Xiang-yang2, ZHAO Cong-cong2, ZHANG Li-guo3, ZHONG Wei-zhang1
1. School of Environmental Science and Engineering, Hebei University of Science & Technology, Shijiazhuang 050018, China; 2. School of Civil Engineering, Hebei University of Science & Technology, Shijiazhuang 050018, China; 3. College of Environment and Resource, Shanxi University, Taiyuan 030006, China
Abstract：The effect of different sludge concentration (MLSS) on denitrification efficiency and microbial community structure in A/O process for starch industry wastewater treatment were investigated. The 16S rDNA Miseq high throughput sequencing technique was used for bacterial commnutiy analysis. The results showed that the average removal rate of ammonia nitrogen (NH4+-N) were respectively 90.2% and 67.2% when the concentration of sludge were 4066mg/L and 2985mg/L. The dominant bacteria were Proteobacteria, Bacteroidetes, Chloroflexi and Firmicutes in both of the pools; while the average abundance ratio of Proteobacteria and Bacteroides were 40.65% and 30.28% in the pool No. 1,25.25% and 33.27% in the pool No. 2respectively. In the functional gene level, all of the eight categories (KEGG, Kyoto Encyclopedia of Genes and Genomes) had a same order. The content of nitrogen metabolism related genes, such as, nitrifying ferments, denitrifying enzymes and ammonifying enzymes genes were higher in the pool No. 1. In this study, the sludge concentration has a significant effect on the bacterial structure, and the high sludge concentration is beneficial to the formation of high efficiency denitrifying bacterial community.
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