Alkaline phosphatase activity of Jialing River estuary
WANG Min1, YUAN Shao-chun1, MENG Cai-xia1, XU Wei1, ZHANG Teng-can2
1. Key Laboratory of Hydraulic and Waterway Engineering of the Ministry of Education, Chongqing Jiaotong University, Chongqing 400074, China;
2. Chengdu Municipal Engineering Design Institute, Chengdu 610015, China
This paper investigated the interrelationship between phosphorus status, change of total alkaline phosphatase activities (TAPA), and algae bloom of the Jialing River Chongqing section from 2011 to 2016. The changes of TAPA, ALGAE and SRP were summarized through in-situ experiments. The activity parameters of alkaline phosphatase were determined, and the ecological significance of enzyme activity in algal bloom was discussed. The results showed that Soluble Reactive Phosphorus (SRP) was the main source of phosphorus for algae growth. The lowest SRP detected as 0.012~0.021mg/L was observed during the critical period of algae bloom, and the highest TAPA was 10.503~11.587nmol/L/min. The variations of characteristic parameters Vm and the Michaelis~Menten constant Km validated that alkaline phosphatase had a higher affinity for substrate and the hydrolysis rate of the enzyme increased significantly during the critical period of algae bloom. It is also found that alkaline phosphatase significantly promoted the transformation of other forms of phosphorus into SRP, thus supplying adequate SRP to the growth of microorganisms such as algae.
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