A novel heterotrophic combining sulfur-based autotrophic ABR reactor was constructed to treatthe high concentration of perchlorate (ClO4-) wastewater.The ClO4- removal, the sulfate (SO42-) production, the biomass and EPS changes under different ClO4- concentrationswereexplored. The results showed that the ClO4- removalefficiency reached to 99.60% at HRT of 12h and the influent ClO4- concentration of 300mg/L, the effluent SO42- concentration was approximately stabilized at 150mg/L.The influent pH was approximately 7.8~8.0. With ClO4- concentration increasing, the effluent pH of the heterotrophic unit was increased to 8.0~8.3, while the effluent pH of the autotrophic unit was decreased to 6.6~6.9.Therefore, the combined heterotrophic and sulfur-based autotrophic process can achieve an acid-base balance.Moreover, the more EPScontent wasproduced by microorganism when influent perchlorate concentration increased gradually in the process.And the contentof EPS in the first compartment of the heterotrophic unit was maximum, which reachedto 102.46mg/(g·vss). The secretion of EPS can not only form a protective layer to resist external pressure, but also play a role in the reserve of carbon sources and energy.
李昆, 李海波, 李瑶峰, 林心仪, 李宝鑫, 宋圆圆, 郭建博. 异养协同硫自养ABR系统还原高浓度高氯酸盐[J]. 中国环境科学, 2018, 38(11): 4153-4158.
LI Kun, LI Hai-bo, LI Yao-feng, LIN Xin-yi, LI Bao-xin, SONG Yuan-yuan, GUO Jian-bo. Heterotrophic combiningsulfur-based autotrophic process reduction of high concentration perchlorate using ananaerobic baffle reactor. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(11): 4153-4158.
Atikovic E, Suidan M T, Maloney S W. Anaerobic treatment of army ammunition production wastewater containing perchlorate and RDX[J]. Chemosphere, 2008,72(11):1643-1648.
Zhu Y, Gao N, Chu W, et al. Bacterial reduction of highly concentrated perchlorate:Kinetics and influence of co-existing electron acceptors, temperature, pH and electron donors[J]. Chemosphere, 2016,148(1):188-194.
Nor S J, Lee S H, Cho K S, et al. Microbial treatment of high-strength perchlorate wastewater[J]. Bioresource Technology, 2011,102(2):835-841.
Srinivasan R, Sorial G A, Srinivasan R. Treatment of perchlorate in drinking water:A critical review[J]. Separation & Purification Technology, 2009,69(1):7-21.
Baidas S, Gao B, Meng X. Perchlorate removal by quaternary amine modified reed[J]. Journal of Hazardous Materials, 2011,189(1/2):54.
Sahinkaya E, Dursun N. Sulfur-oxidizing autotrophic and mixotrophic denitrification processes for drinking water treatment:elimination of excess sulfate production and alkalinity requirement[J]. Chemosphere, 2012,89(2):144-149.
Guerrero L, Aguirre J P, Muñoz M A, et al. Autotrophic and heterotrophic denitrification for simultaneous removal of nitrogen, sulfur and organic matter[J]. Environmental Letters, 2016,51(8):1-6.
Liu H, Jiang W, Wan D, et al. Study of a combined heterotrophic and sulfur autotrophic denitrification technology for removal of nitrate in water[J]. Journal of Hazardous Materials, 2009,169(1):23-28.
Sahinkaya E, Dursun N, Kilic A, et al. Simultaneous heterotrophic and sulfur-oxidizing autotrophic denitrification process for drinking water treatment:control of sulfate production[J]. Water Research, 2011, 45(20):6661-6667.
Wan D, Liu Y, Wang Y, et al. Simultaneous bio-autotrophic reduction of perchlorate and nitrate in a sulfur packed bed reactor:Kinetics and bacterial community structure[J]. Water Research, 2017,108:280-292.
Capua F D, Milone I, Lakaniemi A M, et al. High-rate autotrophic denitrification in a fluidized-bed reactor at psychrophilic temperatures[J]. Chemical Engineering Journal, 2017,313(C):591-598.
Oh S E, Yoo Y B, Young J C, et al. Effect of organics on sulfur-utilizing autotrophic denitrification under mixotrophic conditions[J]. Journal of Biotechnology, 2001,92(1):1-8.
Chang Y J, Chang Y T, Hung C H, et al. Microbial community analysis of anaerobic bio-corrosion in different ORP profiles[J]. International Biodeterioration & Biodegradation, 2014,95:93-101.
Lackner S, Lindenblatt C, Horn H. ‘Swinging ORP’ as operation strategy for stable reject water treatment by nitritation-anammox in sequencing batch reactors[J]. Chemical Engineering Journal, 2012, 180(7):190-196.
You G, Hou J, Yi X, et al. Effects of CeO2, nanoparticles on production and physicochemical characteristics of extracellular polymeric substances in biofilms in sequencing batch biofilm reactor[J]. Bioresource Technology, 2015,194:91-98.
Wang Z, Gao M, Wei J, et al. Extracellular polymeric substances, microbial activity and microbial community of biofilm and suspended sludge at different divalent cadmium concentrations[J]. Bioresource Technology, 2016,205:213-221.
He J, Yang P, Zhang W, et al. Characterization of changes in floc morphology, extracellular polymeric substances and heavy metals speciation of anaerobically digested biosolid under treatment with a novel chelated-Fe2+ catalyzed Fenton process[J]. Bioresource Technology, 2017,243:641-651.
Shi Y, Huang J, Zeng G, et al. Exploiting extracellular polymeric substances (EPS) controlling strategies for performance enhancement of biological wastewater treatments:An overview[J]. Chemosphere, 2017,180:396-411.