浒苔生长-衰亡过程中氮形态的迁移转化过程

摘要
图/表
参考文献(52)
相关文章 (8)

全文: PDF (536 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要

针对浒苔氮吸收代谢机制，通过连续培养实验研究浒苔生长-衰亡过程中不同形态氮在藻体和培养液中的分布和迁移.结果表明，浒苔在生长期吸收NO₃^--N（17.37μmol/（g·d））而衰亡期向胞外释放NH₄⁺-N（0.84μmol/（g·d））；在生长期胞内NO₃^--N占无机氮（DIN）73.75%~92.15%，在衰亡期NH₄⁺-N占无机氮60.87%~92.13%；培养液中溶解有机氮（DON）浓度持续增加，其中<1kDa组分占64%~98%，> 1kDa组分占2%~36%.在培养期藻体细胞吸收DIN速率平均为8.96μmol/（g·d），释放DON的平均迁移速率约为59.57μmol/（g·d）.通过收支模型计算，浒苔将近60% DIN转化为以<1kDa组分占优的DON并分泌到胞外.由此可以推测，近海浒苔爆发可以在月际尺度上对水体生源要素结构产生显著影响.

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	张鹏燕
	严振伟
	钟晓松
	金月梅
	晏茂军
	于继凯
	辛宇
	刘涛

关键词 ：浒苔, 氮形态, 切向超滤

Abstract：

To investigate the mechanism of nitrogen uptake and metabolism in U. prolifera, the distribution and migration of different forms of nitrogen in culture media and algea cells during the growth-decay process was studied with continuous culture experiments. U. prolifera absorbed NO₃^--N (17.37μmol/(g·d)) into the cell during the growth phase while released NH₄⁺-N (0.84μmol/(g·d)) to the extracellular during the decay phase. In algae cell, up to 73.75%~92.15% of dissolved inorganic nitrogen (DIN) was NO₃^--N during the growth phase, while 60.87%~92.13% was NH₄⁺-N during the decay period. The concentration of dissolved organic nitrogen (DON) in the culture media increased continually during the culture period, with < 1kDa component accounting for 64%~98% and > 1kDa component accounting for 2%~36%. The average migration rate of DIN was about 8.96μmol/(g·d), and the average release rate of DON was about 59.57μmol/(g·d). Nearly 60% DIN was converted into DON and secreted extracellularly, most of which was < 1kDa component. It can be speculated that the outburst of U. prolifera impacts the structure of biological elements significantly on the monthly scale.

Key words： Ulva prolifera nitrogen forms tangential flow ultrafiltration

收稿日期: 2018-10-08

PACS:

X142

基金资助:

国家自然科学基金资助面上项目（41576082）；青岛海洋科学与技术国家实验室"鳌山人才"计划项目（2015ASTP-OS08）；国家重点研发计划项目（2016YFC1402101）；中央高校基本科研业务费专项（201762032）

通讯作者: 辛宇,讲师,xinyu312@ouc.edu.cn;刘涛,教授,liutao@ouc.edu.cn E-mail: xinyu312@ouc.edu.cn;liutao@ouc.edu.cn

作者简介: 张鹏燕(1989-),女,山东青岛人,中国海洋大学海洋生命学院博士,主要从事浮游植物氮循环的研究.发表论文2篇.

引用本文:

张鹏燕, 严振伟, 钟晓松, 金月梅, 晏茂军, 于继凯, 辛宇, 刘涛. 浒苔生长-衰亡过程中氮形态的迁移转化过程[J]. 中国环境科学, 2019, 39(5): 1967-1976. ZHAGN Peng-yan, YAN Zhen-wei, ZHONG Xiao-song, JIN Yue-mei, YAN Mao-jun, YU Ji-kai, XIN Yu, LIU Tao. Transformation and migration of nitrogen forms during the growth-decay of Ulva Prolifera. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(5): 1967-1976.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2019/V39/I5/1967

[1]	林雨霏,杨阳,王国善,等.浒苔绿潮灾害损失调查与评估方法构建[J]. 海洋环境科学, 2018,37(3):452-456 Lin Y F, Yang Y, Wang G S, et al. Establishment of the losses investigation and assessment method of green tide (Ulva prolifera) disaster[J]. Marine Environmental Science, 2018,37(3):452-456.
[2]	国家海洋局.《2009年中国海洋灾害公报》[J]. 海洋开发与管理, 2010,27(2):70. State Oceanic Administration. 2009 China marine disaster bulletin[J]. Ocean Development and Management, 2010,27(2):70.
[3]	Liu D, Keesing J K, He P, et al. The world's largest macroalgal bloom in the Yellow Sea, China:formation and implications[J]. Estuarine, Coastal and Shelf Science, 2013,129:2-10.
[4]	Wang C, Yu R C, Zhou M J. Effects of the decomposing green macroalga Ulva (Enteromorpha) prolifera on the growth of four red-tide species[J]. Harmful Algae, 2012,16:12-19.
[5]	王婷,石晓勇,张传松,等.2008年黄海浒苔绿潮爆发区营养盐浓度变化及分布特征[J]. 海洋通报(中文版), 2011,30(5):578-582. Wang T, Shi X Y, Zhang C S, et al. Variations in nutrient concentration and distribution for green tide of Enteromorpha prolifera occurrence areas of the Yellow Sea in 2008[J]. Marine Science Bulletin (Chinese version), 2011,30(5):578-582.
[6]	高嵩,范士亮,韩秀荣,等.浒苔绿潮与南黄海近岸海域水质的关系[J]. 中国环境科学, 2014,34(1):213-218. Gao S, Fan S L, Han X R, et al. Relations of Enteromorpha prolifera blooms with temperature, salinity, dissolved oxygen and pH in the Southern Yellow Sea[J]. China Environmental Science, 2014,34(1):213-218.
[7]	李瑞香,吴晓文,韦钦胜,等.不同营养盐条件下浒苔的生长[J]. 海洋科学进展, 2009,27(2):211-216. Li R X, Wu X W, Wei Q S, et al. Growth of Enteromorpha Prolifera under different uutrient conditions[J]. Advances in Marine Science, 2009,27(2):211-216.
[8]	Li H, Zhang Y, Han X, et al. Growth responses of Ulva prolifera to inorganic and organic nutrients:Implications for macroalgal blooms in the southern Yellow Sea, China[J]. Scientific reports, 2016,6:26498.
[9]	吴晓文,李瑞香,徐宗军,等.营养盐对浒苔生长影响的围隔生态实验[J]. 海洋科学进展, 2010,28(4):538-544. Wu X W, Li R X, Xu Z J, et al. Mesocosm experiments of nutrient effects on enteromorpha prolifera growth[J]. Advances in Marine Science, 2010,28(4):538-544.
[10]	吴婷,赵乐毅,刘浩涤,等.浒苔对营养盐影响的初步研究[J]. 海洋环境科学, 2013,32(3):347-352. Wu T, Zhao LY, Liu H D, et al. Preliminary study on the influence of Enteromorpha prolifera on nutrients[J]. Marine Environmental Science, 2013,32(3):347-352.
[11]	史华明.浒苔对氮磷吸收和释放及与中肋骨条藻竞争关系的初步研究[D]. 青岛:中国海洋大学, 2009. Shi H M. The preliminary study of Enteromorpha absorption and release properties of nitrogen and phosphorus and the competition with Skeletonema costatum[D]. Qing Dao:Ocean University of China, 2009.
[12]	Guidone M, Thornber C S. Examination of Ulva bloom species richness and relative abundance reveals two cryptically co-occurring bloom species in Narragansett Bay, Rhode Island[J]. Harmful Algae, 2013,24:1-9.
[13]	刘湘庆,王宗灵,辛明,等.浒苔衰亡过程中营养盐的释放过程及规律[J]. 海洋环境科学, 2016,35(6):801-805+813. Liu X Q, Wang Z L, Xin M, et al. Study on process of nutrient release during the decay of Ulva Prolifera[J]. Marine Environmental Science, 2016,35(6):801-805+813.
[14]	李鸿妹.营养盐与黄海浒苔绿潮暴发关系的探究[D]. 青岛:中国海洋大学, 2015. Li H M. Study on the relationship between nutrient salt and yellow sea margin green tide[D]. Qingdao:Ocean University of China,2015.
[15]	李俭平.浒苔对氮营养盐的响应及其氮营养盐吸收动力学和生理生态研究[D]. 青岛:中国科学院研究生院(海洋研究所), 2011. Li J P. The response to nitrogen nutrient, and the uptake kinetics mechanism of nitrogen and ecophysiological analysis of Enteromorpha prolifera[D]. Qingdao:Graduate school of Chinese academy of sciences (institute of oceanography), 2011.
[16]	高嵩,石晓勇,王婷.浒苔绿潮与苏北近岸海域营养盐浓度的关系研究[J]. 环境科学, 2012,33(7):2204-2209. Gao S, Shi X Y, Wang T. Variation of nutrient concentrations at the inshore coastal area of northern Jiangsu Province and the occurrence of Green Tide caused by Enteromorpha prolifera[J]. Environmental Science, 2012,33(7):2204-2209.
[17]	Shi X, Qi M, Tang H, et al. Spatial and temporal nutrient variations in the Yellow Sea and their effects on Ulva prolifera blooms[J]. Estuarine, Coastal and Shelf Science, 2015,163:36-43.
[18]	叶静,张喆,李富超,等.大型绿藻浒苔转化表达系统选择标记的筛选[J]. 生物技术通报, 2006,3:63-67. Ye J, Zhang J, Li F C, et al. Screening of selectable marker for transformation and expression system of green seaweed Enteromorpha Clathrata[J]. Biotechnology Bulletin, 2006,3:63-67.
[19]	Zhu M, Liu Z, Shao H, et al. Effects of nitrogen and phosphate enrichment on the activity of nitrate reductase of Ulva prolifera in coastal zone[J]. Acta Physiologiae Plantarum, 2016,38(7):169.
[20]	Guillard R R L, Ryther J H. Studies of marine planktonic diatoms:I. Cyclotella nana Hustedt, and Detonula confervacea (Cleve) Gran[J]. Canadian journal of microbiology, 1962,8(2):229-239.
[21]	Liu S M, Zhang J, Chen H T, et al. Factors influencing nutrient dynamics in the eutrophic Jiaozhou Bay, North China[J]. Progress in Oceanography, 2005,66(1):66-85.
[22]	Xu Z, Liu S, Xiang R, et al. Live benthic foraminifera in the Yellow Sea and the East China Sea:vertical distribution, nitrate storage, and potential denitrification[J]. Marine Ecology Progress Series, 2017, 571:65-81.
[23]	Corzo A, Niell F X. Inorganic nitrogen metabolism inUlva rigida illuminated with blue light[J]. Marine Biology, 1992,112(2):223-228.
[24]	Benner R, Pakulski J D, McCarthy M, et al. Bulk chemical characteristics of dissolved organic matter in the ocean[J]. Science, 1992,255(5051):1561-1564.
[25]	Martin J M, Dai M H, Cauwet G. Significance of colloids in the biogeochemical cycling of organic carbon and trace metals in the Venice Lagoon (Italy)[J]. Limnology and Oceanography, 1995,40(1):119-131.
[26]	Fong P, Fong J J, Fong C R. Growth, nutrient storage, and release of dissolved organic nitrogen by Enteromorpha intestinalis in response to pulses of nitrogen and phosphorus[J]. Aquatic Botany, 2004,78(1):83-95.
[27]	Zhang T, Wang X. Release and microbial degradation of dissolved organic matter (DOM) from the macroalgae Ulva prolifera[J]. Marine pollution bulletin, 2017,125(1/2):192-198.
[28]	Lin H, Jiang P, Zhang J, et al. Genetic and marine cyclonic eddy analyses on the largest macroalgal bloom in the world[J]. Environmental science & technology, 2011,45(14):5996-6002.
[29]	Wang Z, Xiao J, Fan S, et al. Who made the world's largest green tide in China?-an integrated study on the initiation and early development of the green tide in Yellow Sea[J]. Limnology and Oceanography, 2015,60(4):1105-1117.
[30]	Li H, Zhang Y, Tang H, et al. Spatiotemporal variations of inorganic nutrients along the Jiangsu coast, China, and the occurrence of macroalgal blooms (green tides) in the southern Yellow Sea[J]. Harmful algae, 2017,63:164-172.
[31]	Solomonson L P, Barber M J. Assimilatory nitrate reductase:functional properties and regulation[J]. Annual review of plant biology, 1990,41(1):225-253.
[32]	Suzuki A, Gadal P. Glutamate synthase from rice leaves[J]. Plant Physiology, 1982,69(4):848-852.
[33]	Koch G W, Schulze E D, Percival F, et al. The nitrogen balance of Raphanus sativus x raphanistrum plants. Ⅱ. Growth, nitrogen redistribution and photosynthesis under NO3- deprivation[J]. Plant, Cell & Environment, 1988,11(8):755-767.
[34]	Chimney M J, Pietro K C. Decomposition of macrophyte litter in a subtropical constructed wetland in south Florida (USA)[J]. ecological engineering, 2006,27(4):301-321.
[35]	Vaquer-Sunyer R, Conley D J, Muthusamy S, et al. Dissolved organic nitrogen inputs from wastewater treatment plant effluents increase responses of planktonic metabolic rates to warming[J]. Environmental science & technology, 2015,49(19):11411-11420.
[36]	李文朝,陈开宁,吴庆龙,等.东太湖水生植物生物质腐烂分解实验[J]. 湖泊科学, 2001,(4):331-336. Li W C, Chen K N, Wu Q L, et al. Expeimental studies on decomposition process of aquatic plant material from East Taihu Lake[J]. Journal of Lake Scicences, 2001,(4):331-336.
[37]	Tyler A C, McGlathery K J, Anderson I C. Macroalgae mediation of dissolved organic nitrogen fluxes in a temperate coastal lagoon[J]. Estuarine, Coastal and Shelf Science, 2001,53(2):155-168.
[38]	Thornton D C O. Dissolved organic matter (DOM) release by phytoplankton in the contemporary and future ocean[J]. European Journal of Phycology, 2014,49(1):20-46.
[39]	Sharp J H. Excretion of organic matter by marine phytoplankton:Do healthy cells do it?[J]. Limnology and Oceanography, 1977,22(3):381-399.
[40]	Nagata T. Production mechanisms of dissolved organic matter[M]. New York, Microbial ecology of the oceans, 2000:121-152.
[41]	Veldhuis M J W, Kraay G W, Timmermans K R. Cell death in phytoplankton:correlation between changes in membrane permeability, photosynthetic activity, pigmentation and growth[J]. European Journal of Phycology, 2001,36(2):167-177.
[42]	Berman F I, Rosenberg G, Levitan O, et al. Coupling between autocatalytic cell death and transparent exopolymeric particle production in the marine cyanobacterium Trichodesmium[J]. Environmental microbiology, 2007,9(6):1415-1422.
[43]	Timmermans K R, Veldhuis M J W, Brussaard C P D. Cell death in three marine diatom species in response to different irradiance levels, silicate, or iron concentrations[J]. Aquatic microbial ecology, 2007, 46(3):253-261.
[44]	Franklin D J, Airs R L, Fernandes M, et al. Identification of senescence and death in Emiliania huxleyi and Thalassiosira pseudonana:Cell staining, chlorophyll alterations, and dimethylsulfoniopropionate (DMSP) metabolism[J]. Limnology and Oceanography, 2012,57(1):305-317.
[45]	Lee C, Howarth R W, Howes B L. Sterols in decomposing Spartina alterniflora and the use of ergosterol in estimating the contribution of fungi to detrital nitrogen 1[J]. Limnology and Oceanography, 1980, 25(2):290-303.
[46]	Valiela I, Teal J M, Allen S D, et al. Decomposition in salt marsh ecosystems:the phases and major factors affecting disappearance of above-ground organic matter[J]. Journal of Experimental Marine Biology and Ecology, 1985,89(1):29-54.
[47]	Hicks R E, Lee C, Marinucci A C. Loss and recycling of amino acids and protein from smooth cordgrass (Spartina alterniflora) litter[J]. Estuaries, 1991,14(4):430-439.
[48]	White D S, Howes B L. Long-term 15N-nitrogen retention in the vegetated sediments of a New England salt marsh[J]. Limnology and Oceanography, 1994,39(8):1878-1892.
[49]	Lee D Y, Rhee G Y. Kinetics of cell death in the cyanobacterium anabaena flos-aquae and the production of dissolved organic carbon[J]. Journal of Phycology, 1997,33(6):991-998.
[50]	丁月旻.黄海浒苔绿潮中生源要素的迁移转化及对生态环境的影响[D]. 青岛:中国科学院研究生院(海洋研究所), 2014. Ding Y M. Impacts of Ulva(enteromorpha) prolifera in the green tide on the Yellow Sea ecological environmen-implications from migration and tranformation of biogenic elements[D]. Qingdao:Graduate school of Chinese Academy of Sciences (Institute of Oceanography), 2015.
[51]	Zhou Y, Tan L, Pang Q, et al. Influence of nutrients pollution on the growth and organic matter output of Ulva prolifera in the southern Yellow Sea, China[J]. Marine Pollution bulletin, 2015,95(1):107-114.
[52]	Liu X, Li Y, Wang Z, et al. Cruise observation of Ulva prolifera bloom in the southern Yellow Sea, China[J]. Estuarine, Coastal and Shelf Science, 2015,163:17-22.