Effect of FNA on microorganism community structures of partial nitrification sludge
GAO Chun-di, ZHAO Nan, AN Ran, HAN Hui, ZHANG Na, PENG Yong-zhen
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China
The long-term effects on ammonia-oxidizing bacteria (AOB), filamentous bacteria and microorganism community structures in partial nitrification sludge was investigated by adding a pretreatment unit of free nitrous acid (FNA) in the sequence bath reactor (SBR) for three days at 1.2mg HNO2-N/L for 4.5. The results showed that FNA had the short-time effect on AOB, Candidatus_Microthrix and Cytophagaceae of the dominant filamentous bacteria were also decreased from 5.1% and 1.1% to 0.78% and almost invisible. Sludge volume index (SVI) maintained at 110mL/g dropping from 281mL/g, and the nitrite accumulation rate (NAR) was kept at around 90%, indicating the partial nitrification was not undermined. Furthermore, High-throughput sequencing results showed that the diversity and uniformity of microorganism community decreased. However, the proliferation of Thauera and Ottowia increased to 5.58% and 7.82%. Simultaneous nitrification and denitrification (SND) had significant effects, and the total nitrogen removal rate was nevertheless maintained more than 60% even only with partial nitrification.
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