Neurotoxic effect of environmental persistent free radicals in rice biochar to Caenorhabditis elegans
ZHANG Xu-chao1,2, CHEN Yi1,2, HU Die1, ZHAO Li1,2, WANG Lin1,2, WU Min1,2
1. Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China;
2. Yunnan Provincial Key Lab of Carbon Sequestration and Pollution Control in Soils, Kunming 650500, China
As an effective soil amendment, biochars have attracted increasing research attention, while their environmental risks have not been fully explored. Studies on the effects of biochars on ecosystem, especially on soil invertebrates, are still scarce. The model organism, Caenorhabditis elegans (C. elegans, nematode), was used to assess the potential neurotoxicity of biochar. The neurobehaviors of C. elegans, including their body bends, relative move length, defecation, touch response and chemical index, were investigated after 24h-exposed in biochars, the washed biochars and biochar supernatants. The contents of metallic and non-metallic elements, biochar physicochemical properties and the environmentally persistent free radicals (EPFRs) were characterized. The results showed a hormesis behavior of C. elegans when exposed in biochar particles with high EPFRs intensity. No significant effect to C. elegans was observed when exposed in supernatants. Compared to the other endogenous pollutants in biochars, EPFRs have a potential neurotoxic effects to nematodes. This study provides a new angle to assess the potential environmental risks of biochar application.
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