Effects of multiple factors on the process of ANAMMOX bacteria strengthening nitrate reduction by zero-valent iron
SONG Ge1, ZHANG Wen-jing1,2,3, BI Zhen1,2,3, HUANG Yong1,2,3, DONG Shi-yu1,3
1. School of Environment Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; 2. National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou 215009, China; 3. Institute of Environmental Biotechnology, Suzhou University of Science and Technology, Suzhou 215009, China
Abstract:Effects of iron form, pH, temperature and Fe/N on the process of ANAMMOX bacteria strengthening nitrate reduction by zero-valent iron (Fe0) were studied by single factor control experiment combined with Central Composite Design (CCD) methodology. The results demonstrated that the test with nano-Fe0 addition showed the highest nitrate removal efficiency compared with the iron powder and iron filings addition. According to the CCD analysis, the temperature and Fe/N had significant effects on the nitrate removal efficiency, while the effect of pH was weak. The optimum reaction conditions predicted based on CCD analysis were initial pH of 4.00, temperature of 35.00℃, and Fe/N of 38.23. The predictive nitrate removal efficiency was 94.70%, which was closed to the actual experimental value of 88.99%.
宋歌, 张文静, 毕贞, 黄勇, 董石语. 多因素对ANAMMOX菌利用零价铁还原硝酸盐过程影响[J]. 中国环境科学, 2019, 39(11): 4666-4672.
SONG Ge, ZHANG Wen-jing, BI Zhen, HUANG Yong, DONG Shi-yu. Effects of multiple factors on the process of ANAMMOX bacteria strengthening nitrate reduction by zero-valent iron. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(11): 4666-4672.
Oshiki M, Satoh H, Okabe S, et al. Ecology and physiology of anaerobic ammonium oxidizing bacteria[J]. Environmental Microbiology, 2016,18(9):2784-2796.
[2]
Feng Y, Zhao Y P, Guo Y Z, et al. Microbial transcript and metabolome analysis uncover discrepant metabolic pathways in autotrophic and mixotrophic anammox consortia[J]. Water Research, 2018,128:402-411.
[3]
周正,王凡,林兴,等.中试一体式部分亚硝化-厌氧氨氧化反应器的启动与区域特性[J]. 环境科学, 2018,39(3):1301-1308. Zhou Z, Wang F, Lin X, et al. Start-up and regional characteristics of a pilot-scale integrated PN-ANAMMOX reactor[J]. Environmental Science, 2018,39(3):1301-1308.
[4]
Lackner S, Gilbert E M, Vlaeminck S E, et al. Full-scale partial nitritation/anammox experiences-An application survey[J]. Water Research, 2014,55(10):292-303.
[5]
Soliman M, Eldyasti A. Ammonia-oxidizing bacteria (AOB):opportunities and applications-a review[J]. Reviews in Environmental Science and Bio/Technology, 2018,17(2):285-321.
[6]
Cao Y, Hong K B, van Loosdrecht M C, et al. Mainstream partial nitritation and anammox in a 200,000m3/day activated sludge process in Singapore:scale-down by using laboratory fed-batch reactor[J]. Water Science and Technology, 2016,74(1):48-56.
[7]
Cao Y, Hong K B, Zhou Y, et al. Mainstream partial nitritation/anammox nitrogen removal process in the largest water reclamation plant in Singapore[J]. Journal of Beijing University of Technology, 2015,41(10):1441-1454.
[8]
付昆明,付巢,李慧,等.主流厌氧氨氧化工艺的运行优化及微生物的群落变迁[J]. 环境科学, 2018,39(12):5596-5604. Fu K M, Fu C, Li H, et al. Optimization of the mainstream anaerobic ammonia oxidation process and its changes of the microbial community[J]. Environmental Science, 2018,39(12):5596-5604.
[9]
Oshiki M, Ishii S, Yoshida K, et al. Nitrate-dependent ferrous iron oxidation by anaerobic ammonia oxidation (ANAMMOX) bacteria[J]. Applied and Environmental Microbiology, 2013,79(13):4087-4093.
[10]
周健,黄勇,袁怡,等. ANAMMOX菌利用零价铁转化氨和硝酸盐实验[J]. 环境科学, 2015,36(12):4546-4552. Zhou J, Huang Y, Yuan Y, et al. Simultaneous biotransformation of ammonium and nitrate via zero-valent iron on anaerobic conditions[J]. Environmental Science, 2015,36(12):4546-4552.
[11]
国家环境保护总局.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社, 2002.
[12]
Su J F, Shao S C, Huang T L, et al. Anaerobic nitrate-dependent iron(II) oxidation by a novel autotrophic bacterium, Pseudomonas sp. SZF15[J]. Journal of Environmental Chemical Engineering, 2015,3:2187-2193.
[13]
Zhang Q, Ji F F, Xu X Y, et al. Optimization of nitrate removal from wastewater with a low C/N ratio using solid-phase denitrification[J]. Environmental Science and Pollution Research, 2016,23:698-708.
[14]
Wang H B, Hu C, Shen Y, et al. Response of microorganisms in biofilm to sulfadiazine and ciprofloxacin in drinking water distribution systems[J]. Chemosphere, 2019,218:197-204.
[15]
Wang S S, Zhou Y X, Gao B, et al. The sorptive and reductive capacities of biochar supported nanoscaled zero-valent iron(nZVI) in relation to its crystallite size[J]. Chemosphere, 2017,186:495-500.
[16]
Xu J, Cao Z, Wang Y, et al. Distributing sulfidized nanoscale zero-valent iron onto phosphorus-functionalized biochar for enhanced removal of antibiotic florfenicol[J]. Chemical Engineering Journal, 2019,359:713-722.
[17]
Zhang Y P, Douglas G B, Pu L, et al. Zero-valent iron-facilitated reduction of nitrate:Chemical kinetics and reaction pathways[J]. Science of the Total Environment, 2017,598:1140-1150.
[18]
Sun Y K, Li J X, Huang T L, et al. The influences of iron characteristics, operating conditions and solution chemistry on contaminants removal by zero-valent iron:A review[J]. Water Research, 2016,100:277-295.
[19]
Han L C, Yang L, Wang H B, et al. Sustaining reactivity of Fe0 for nitrate reduction via electron transfer between dissolved Fe2+ and surface iron oxides[J]. Journal of Hazaradous Materials, 2016,308:208-215.
[20]
Liu H B, Chen Z H, Guan Y N, et al. Role and application of iron in water treatment for nitrogen removal:A review[J]. Chemosphere, 2018,204:51-62.
[21]
李海玲,李东,张杰,等.调控温度和沉降时间实现ANAMMOX颗粒快速启动及其稳定运行[J]. 环境科学, 2019,2:1-11. Li H L, Li D, Zhang J, et al. Adjusting temperature and settling time to achieve ANAMMOX particles rapid start-up and stable operation[J]. Environmental Science, 2019,2:1-11.
[22]
周同,于德爽,李津,等.温度对海洋厌氧氨氧化菌脱氮效能的影响[J]. 环境科学, 2017,38(5):2044-2051. Zhou T, Yu D S, Li J, et al. Effect of temperature on nitrogen removal performance of marine anaerobic ammonium axidizing bacteria[J]. Environmental Science, 2017,38(5):2044-2051.
[23]
陈方敏,金润,袁砚,等.温度和pH值对铁盐型氨氧化过程氮素转化的影响[J]. 环境科学, 2018,39(9):4289-4293. Chen F M, Jin R, Yuan Y, et al. Effect of temperature and pH on nitrogen conversion in Feammox process[J]. Environmental Science, 2018,39(9):4289-4293.
[24]
Zhang Y P, Douglas G B, Pu L, et al. Zero-valent iron-facilitated reduction of nitrate:Chemical kinetics and reaction pathways[J]. Science of the Total Environment, 2017,598:1140-1150.
[25]
Sun Y K, Li J X, Huang T L, et al. The influences of iron characteristics, operating conditions and solution chemistry on contaminants removal by zero-valent iron:A review[J]. Water Research, 2016,100:277-295.