Different effect of EPS in layers and its components on settleability of heterogenic bulking activated sludge with condition of nutritional imbalance
HU Xiao-bing1,2, WEI Jing-yun1, LIN Rui1, WANG Kun1, CHANG Jing1, GU Xian-jing1, ZHANG Lin1, ZHONG Mei-ying1
1. College of Architectural Engineering, Anhui University of Technology, Ma'anshan 243032, China;
2. Engineering Research Center of Water Purification and Utilization Technology Based on Biofilm Process, Ministry of Education, Ma'anshan 243032, China
To explore the different effects of extracellular polymeric substance (EPS) on settleability of bulking sludge, the influent conditions changed with shortage of elements P and N for culture different bulking sludges, the EPS in different layers and their components in bulking sludges were analyzed. The results showed that no matter in the non-filamentous viscous bulking sludge or in the filamentous bulking sludge, the contents of Total-PS, of EPS in the outer layer(S-PS、S-PN), and of EPS in the middle layer(L-PS) were higher than those in the normal activated sludge. Polysaccharide content in EPS of all layers in non-filamentous viscous bulking sludge was more than that in filamentous bulking sludge, while the contents of PN of all layers in non-filamentous viscous bulking sludge were lower than those in filamentous bulking sludge. Total-EPS ((229±94) mg/g MLSS) in non-filamentous viscous bulking sludge was higher than that in normal activated sludge ((86±16) mg/g MLSS) and filamentous bulking sludge ((108±30) mg/g MLSS). The higher the contents of Total-P, Total-PN in non-filamentous viscous bulking sludge were, the bigger SVI value was and the worse sludge settleability was. Protein had more great effect on the sludge settleability than polysaccharide did. In the view of EPS in layers, the EPS in incompact outer layer(layer S) was the key to the formation of non-filamentous viscous bulking sludge(value of S-PN and SVI had the greatest correlation, r=0.881, P<0.05). Total contents of EPS components, the EPS contents in the different layers but tightly bond T-PN were all weak-negative correlated with SVI.
胡小兵, 韦京云, 林睿, 汪坤, 常静, 顾娴静, 张琳, 钟梅英. 营养失衡下的分层分质EPS对异型膨胀污泥及其沉降性差异影响[J]. 中国环境科学, 2019, 39(12): 5110-5118.
HU Xiao-bing, WEI Jing-yun, LIN Rui, WANG Kun, CHANG Jing, GU Xian-jing, ZHANG Lin, ZHONG Mei-ying. Different effect of EPS in layers and its components on settleability of heterogenic bulking activated sludge with condition of nutritional imbalance. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(12): 5110-5118.
田园.SBR反应器中氮和磷含量对污泥膨胀现象影响研究[D]. 吉林:吉林建筑大学, 2013. Tian Y. Experimental study on the effects of nitrogen and phosphorus content of SBR reactor for sludge bulking[D]. Jilin:Jilin university architecture and civil engineering, 2013.
[2]
梅哈古丽·艾尼瓦尔,姚俊芹,宋文娟,等.膨胀污泥的胞外聚合物组分及其三维荧光光谱分析[J]. 中国给水排水, 2017,33(7):109-117. Mehaguli·A, Yao J, Song W, et al. Components and three-dimension excitation-emission matrix fluorescence spectral characteristics of EPS from bulking Sludge[J]. China Water & Wastewater, 2017,33(7):109-117.
[3]
Liu R, Qi R, Wang J, et al. Phage-host associations in a full-scale activated sludge plant during sludge bulking[J]. Applied Microbiology and Biotechnology, 2017,101(16):6495-6504.
[4]
张振宇,费学宁,焦秀梅,等.微丝菌培养分离、特性标记及调控研究进展[J]. 工业水处理, 2017,37(10):1-5. Zhang Z Y, Fei X N, Jiao X M, et al. Research progress in the characteristics, labelling and regulation of microthrix parvicella[J]. Industrial Water Treatment, 2017,37(10):1-5.
[5]
彭永臻,郭建华.活性污泥膨胀机理、成因及控制[M]. 北京:科学出版社, 2012. Peng Y Z, Guo J H. Mechanism of activated sludge bulking:causes and control[M]. Beijing:Science Press, 2012.
[6]
高春娣,彭永臻,王淑莹.磷缺乏引起的非丝状菌活性污泥膨胀[J]. 中国环境科学, 2002,22(1):40-43. Gao C D, Peng Y Z, Wang S Y. Non-filamentous activated sludge bulking caused by the deficiency of phosphorus[J]. China Environmental Science, 2002,22(1):40-43.
[7]
吴昌永,彭永臻,彭轶. A2O工艺中的污泥膨胀问题及恢复研究[J]. 中国环境科学, 2008,28(12):1074-1078. Chang Y, Peng Y Z, Peng Y. Activated sludge bulking and control in A2O process[J]. China Environmental Science, 2008,28(12):1074-1078.
[8]
吕冬梅,彭永臻,赵伟华,等.A2O-BAF反硝化除磷工艺丝状菌膨胀的发生及控制[J]. 中国环境科学, 2015,35(10):3026-303. Lv D M, Peng Y Z, Zhao W H, et al. Occurrence and control of filamentous bulking in A2O-BAF denitrifying phosphorus removal process. China Environmental Science, 2015,35(10):3026-303.
[9]
刘振超.胞外聚合物对活性污泥沉降性能影响机制研究[D]. 青岛:青岛理工大学, 2015. Liu Z C. Mechanism study on the effect of extracellular polymer substance to the settleability of activated sludge[D]. Qingdao:Qingdao Technological University, 2015.
[10]
Sheng G P, Yu H Q, Li X Y. Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems:A review[J]. Biotechnology Advances, 2010,28(6):882-894.
[11]
Flemming H C, Wingender J. The Biofilm matrix[J]. Nature Reviews Microbiology, 2010,8(9):623-633.
[12]
李昆,李海波,李瑶峰,等.异养协同硫自养ABR系统还原高浓度高氯酸盐[J]. 中国环境科学, 2018,38(11):4153-4158. Li K, Li H B, Li Y F, et al. Heterotrophic combining sulfur-based autotrophic process reduction of high concentration perchlorate using ananaerobic baffle reactor. China Environmental Science, 2018,38(11):4153-4158.
[13]
王然登,程战利,彭永臻,等.强化生物除磷系统中胞外聚合物的特性[J]. 中国环境科学, 2014,34(11):2838-2843. Wang R D, Cheng Z L, Peng Y Z, et al. Characteristics of EPS taken from an enhanced biological phosphorus removal system. China Environmental Science, 2014,34(11):2838-2843.
[14]
Martins A M, Karahan O, Van Loosdrecht MC. Effect of polymeric substrate on sludge settleability[J]. Water Research, 2011,45(1):263-273.
[15]
梅哈古丽·艾尼瓦尔.活性污泥胞外聚合物特性与沉降性能的关系研究[D]. 乌鲁木齐:新疆大学, 2017. Meihaguli·A. Study on the extracellular polymer substance and the settle ability of activated sludge[D]. Wulumuqi:Xinjiang University, 2017.
[16]
Shi Y, Huang J, Zeng G, et al. Exploiting extracellular polymeric substances (EPS) controlling strategies for performance enhancement of biological wastewater treatments:An overview[J]. Chemosphere, 2017,180:396-411.
[17]
Liu H, Fang H H. Characterization of electrostatic binding sites of extracellular polymers by linear programming analysis of Titration Data[J]. Biotechnology & Bioengineering, 2010,80(7):806-811.
[18]
Wang H, Deng H, Ma L, et al. Influence of operating conditions on extracellular polymeric substances and surface properties of sludge flocs[J]. Carbohydrate Polymers, 2013,92(1):510-515.
[19]
胡小兵,叶星,周元凯,等.胞外聚合物对活性污泥吸附生活污水碳源的影响[J]. 环境科学学报, 2016,36(11):4062-4069. Hu X B, Ye X, Zhou Y K, et al. The effect of extracellular polymeric substances on adsorption of the carbon source in sewage by activated sludge[J]. Acta Scientiae Circumstantiae, 2016,36(11):4062-4069.
[20]
Wang B B, Liu X T, Chen J M, et al. Composition and functional group characterization of extracellular polymeric substances (EPS) in activated sludge:the impacts of polymerization degree of proteinaceous substrates[J]. Water Research, 2017,129(1):133-142
[21]
Basuvaraj M, Fein J, Liss S N. Protein and polysaccharide content of tightly and loosely bound extracellular polymeric substances and the development of a granular activated sludge floc[J]. Water Research, 2015,(82):104-117.
[22]
侍宽,薛罡,高品,等.丝状菌膨胀对无纺布生物反应器处理效果及膜污染特征的影响[J]. 环境科学, 2014,35(6):2241-2248. Shi K, Xue G, Gao P, et al. Impacts of filamentous bulking on treatment effect and fouling characteristics of nonwoven bioreactor[J]. Environmental Science, 2014,35(6):2241-2248.
[23]
姚萌,罗红元,谢小青,等.城市污水厂活性污泥胞外聚合物的三维荧光特性分析[J]. 中国环境科学, 2012,32(1):94-99. Yao M, Luo H Y, Xie X Q, et al. Three dimensional excitation emission florescence spectra of extracellular polymer substances in activated sludge of WWTP[J]. China Environmental Science, 2012, 32(1):94-99.
[24]
孙洪伟,陈翠忠,高宇学,等.碳氮比对活性污泥胞外聚合物的长期影响[J]. 中国环境科学, 2018,38(3):950-958. Sun H W, Chen C Z, Gao Y X, et al. Effect of C/N ratio on extracellular polymeric substance (EPS) in the sequencing batch reactor (SBR). China Environmental Science, 2018,38(3):950-958.
[25]
Long X Y, Fang Z D, Tang R, et al. Effect of EPS and cation on settleability of activated sludge[J]. China Water & Wastewater, 2010, 26(13):50-53.
[26]
Zhang L H, Li J, Guo J B, et al. Effect of EPS on activated sludge flocculation ability, settleability and surface properties[J]. CIESC Journal, 2012,63(6):1865-1871.
[27]
俞苗新,潘杨,黄勇,等.活性污泥法合成PHB的研究进展[J]. 水处理技术, 2012,(6):1-6. Yu M X, Pan Y, Huang Y, et al. Research advances in producing PHB by activated process[J]. Technology of Water Treatment, 2012,(6):1-6.
[28]
张淼,彭永臻,张建华,等.进水C/N对A2/O-BCO工艺反硝化除磷特性的影响[J]. 中国环境科学, 2016,36(5):1366-1375. Zhang M, Peng Y Z, Zhang J H. et al. Effect of influent C/N ratios on denitrifying phosphorus removal characteristics in the A2/O-BCO process[J]. China Environmental Science, 2016,36(5):1366-1375.
[29]
郝二成,袁星,阜崴,等.污泥膨胀原因及控制措施研究[J]. 环境工程, 2017,35(7):18-22. Hao E C, Yuan X, Fu W, et al. Research on reason for sludge bulking and its control stategy[J]. Environmental Engineering, 2017,35(7):18-22.
[30]
国家环境保护总局.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社, 2002:200-281. State Environmental Protection Administration. Water and waste water monitoring and analysis method (fourth edition)[M]. Beijing:China Environmental Science Press, 2000:200-281.
[31]
吕文洲,王小宝,刘英,等.预曝气及提取方法对活性污泥胞外聚合物的影响[J]. 中国环境科学, 2018,38(1):150-160. Lv W Z, Wang X B, Liu Y, et al. Effects of pre-aeration and extraction methods on characteristics of extracellular polymeric substances extracted from activated sludge[J]. China Environmental Science, 2018,38(1):150-160.
[32]
刘昌庚,伍斌,谢四才.Fe(Ⅱ)活化过一硫酸盐氧化调理剩余活性污泥[J]. 中国环境科学, 2017,37(10):3794-3799. Liu C G, Wu B, Xie S C, Conditioning of excess activate sludge by Fe(II)-activated peroxymonosulfate oxidation[J]. China Environmental Science, 2017,37(10):3794-3799.
[33]
Miriam W, David G, Wareham, P, et al. The effect of loess addition on the settling ability of activated sludge[J]. Journal of Environmental Science and Health, Part A, 2015,50(7):728-734.
[34]
杨培,王淑莹,顾升波,等.中试SBR长期运行中粘性膨胀现象及原因分析[J]. 环境科学学报, 2010,30(7):1384-1389. Yang P, Wang S Y, Gu S B, et al. The causes of non-filamentous activated-sludge bulking in a long-term pilot-scale sequencing batch reactor(SBR)[J]. Acta Scientiae Circumstantiae, 2010,30(7):1384-1389.
[35]
何志江,赵媛,张源凯,等.活性污泥表面性质对絮凝沉降性能与出水悬浮物的影响[J]. 环境科学, 2016,37(8):3135-3143. He Z J, Zhao Y, Zhang Y K, et al. Influence of activated sludge surface properties on flocculating settling and effluent suspend solid[J]. Environmental Science, 2016,37(8):3135-3143.
[36]
陈滢,彭永臻,刘敏,等.营养物质对污泥沉降性能的影响及污泥膨胀的控制[J]. 环境科学, 2004,25(6):54-58. Chen Y, Peng Y Z, Liu M, et al. Effect of Nutrient on Sludge Settling Property and Bulking Controls[J]. Environmental Science, 2004,25(6):54-58.
[37]
杨雄,彭永臻,宋姬晨,等.进水中碳水化合物分子大小对污泥沉降性能的影响[J]. 中国环境科学, 2015,35(2):448-456. Yang X, Peng Y Z, Song J C, et al. Effect of influent carbohydrates with different molecule-size on sludge settleability[J]. China Environmental Science, 2015,35(2):448-456.
[38]
刘垚.微丝菌型活性污泥膨胀的影响因素与调控方法研究[D]. 北京:清华大学, 2016. Liu G. Study on the Influence Factors and Control Strategies for activated sludge bulking by microthrix parvicella[D]. Beijing:Tsinghua University, 2016.
[39]
He Z J, Zhao Y, Zhang Y K, et al. Influence of activated sludge surface properties on flocculating settling and effluent suspend solid[J]. Environmental Science, 2016,37(8):3135-3143.
[40]
樊鹏超,曾薇,纪兆华,等.城市污水厂活性污泥中胞外聚合物与工艺运行及污泥沉降性能的相关性分析[J]. 环境科学学报, 2017,(8):184-190. Fan P C, Zeng W, Ji Z H, et al. Correlation of extracellular polymeric substance (EPS) in Activated sludge of municipal wastewater treatment plants to process performance and sludge settleability[J]. Acta Scientiae Circumstantiae, 2017,(8):184-190.
[41]
马兴冠,纪文娟,江涛,等.生活污水处理中胞外聚合物对活性污泥絮凝沉降性的影响[J]. 过程工程学报, 2013,13(2):207-211. Ma X G, Ji W J, Jiang T, et al. Effect of extracellular polymeric substances on flocculation-sedimentation during domestic wastewater treatment[J]. The Chinese Journal of Process Engineering. 2013, 13(2):207-211.
