Optimization of salt stress condition for accumulation of triglycerides in Scenedesmus bijuga FACHB-78
WANG Xu1, SUN Xin1, LI Peng-fei1, WANG Jia-le1, CHENG Zhi-wen2
1. Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China;
2. Xianyang Research & Design Institute of, Ceramics, Xianyang 712000, China
The growth morphology, triglyceride accumulation, photosynthetic activity and other characteristics of Scenedesmus bijuga FACHB-78 were studied under the conditions of NaCl concentration ranging from 0 to 45‰, and the optimal salt stress condition was determined. The results showed that with the increase of salinity, the density of algae cells gradually decreased, the ratio of chlorophyll a content to carotenoid content gradually increased, but the accumulation of triglyceride increased first and decreased thereafter. When the salinity was 10‰, the density of algae cells was 26.30% lower than that in normal medium, and the level and content of triglyceride both achieved the maximum values of 250.88mg/L and 33.41%, respectively, which were 97.05% and 82.09% higher than those in normal medium. Additionally, the Photosynthesis was relatively active. For the production of biodiesel, under the stress of salinity of 10‰, Scenedesmus bijuga FACHB-78 cells had greater advantages over algae cells cultured under normal medium.
王垿, 孙昕, 李鹏飞, 王佳乐, 成智文. 双对栅藻FACHB-78甘油三酯积累的盐胁迫条件优化[J]. 中国环境科学, 2019, 39(12): 5248-5253.
WANG Xu, SUN Xin, LI Peng-fei, WANG Jia-le, CHENG Zhi-wen. Optimization of salt stress condition for accumulation of triglycerides in Scenedesmus bijuga FACHB-78. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(12): 5248-5253.
方正,吕德义.微藻制备生物柴油的研究进展[J]. 现代化工, 2017,37(9):57-61. Fang Z, Lü D Y. Research advances in preparation of biological diesel oil by microalgae method[J]. Modern Chemical Industry, 2017,37(9):57-61.
[2]
熊伟,黄云,付乾,等.微藻生物膜营养环境对微藻生长和油脂积累影响[J]. 中国环境科学, 2016,36(8):2463-9. Xiong W, Huang Y, Fu Q, et al. Effect of nutrient solution content of biofilm on algal growth and lipid accumulation[J]. China Environmental Science, 2016,36(8):2463-2469.
[3]
Sajjadi, Chen W Y, Raman AAA, et al. Microalgae lipid and biomass for biofuel production:A comprehensive review on lipid enhancement strategies and their effects on fatty acid composition[J]. Renewable and Sustainable Energy Reviews, 2018,97:200-32.
[4]
Branco V M, Martin S S, Agurto C, et al. Analyzing Phaeodactylum tricornutum lipid profile for biodiesel production[J]. Energy Procedia, 2017,136:369-73.
[5]
Zakery A M A, Bolandnazar S, Oustan S. Effect of salinity and nitrogen on growth, sodium, potassium accumulation, and osmotic adjustment of halophyte Suaedaaegyptiaca (Hasselq.) Zoh[J]. Archives of Agronomy & Soil Science, 2014,60(6):785-92.
[6]
Srivastava G, Nishchal L, Goud V V. Salinity induced lipid production in microalgae and cluster analysis (ICCB 16-BR_047)[J]. Bioresource Technology, 2017,242-252.
[7]
Xia L, Rong J F, Yang H J, et al. NaCl as an effective inducer for lipid accumulation in freshwater microalgae Desmodesmusabundans[J]. Bioresource Technology, 2014,161(11):402-9.
[8]
Venkata M S, Devi M P. Salinity stress induced lipid synthesis to harness biodiesel during dual mode cultivation of mixotrophic microalgae[J]. Bioresource Technology, 2014,165(6):288-94.
[9]
韩松芳,金文标,涂仁杰,等.细菌对城市污水中斜生栅藻生长与产脂的影响[J]. 中国环境科学, 2017,37(10):3867-3872. Han S F, Jin W B, Tu R J, et al. Effects of bacteria on growth and lipid production of Scenedesmus obliquus cultivated in municipal wastewater[J]. China Environmental Science, 2017,37(10):3867-3872.
[10]
王长海,贾顺义.盐度对紫球藻生长及氮磷利用的影响[J]. 哈尔滨工业大学学报, 2009,41(12):194-197. Wang C H, Jia S Y. Effects of salinity on the growth of Porphyridiumcruentum and utilization of nitrate and phosphate[J]. Journal of Harbin Institute of Technology, 2009,41(12):194-197.
[11]
Wu H Q, Miao X L. Biodiesel quality and biochemical changes of microalgae Chlorella pyrenoidosa and Scenedesmus obliquus in response to nitrate levels[J]. BioresourTechnol, 2014,170(170C):421-7.
[12]
Stanier R Y, Kunisawa R, Mandel M, et al. Purification and properties of unicellular blue-green algae (order Chroococcales)[J]. Bacteriological Reviews, 1971,35(2):171.
[13]
Mandotra S K, Kumar P, Suseela M R, et al. Evaluation of fatty acid profile and biodiesel properties of microalga Scenedesmusabundans under the influence of phosphorus, pH and light intensities[J]. Bioresource Technology, 2015,201:222-9.
[14]
许海,陈丹,陈洁,等.氮磷形态与浓度对铜绿微囊藻和斜生栅藻生长的影响[J]. 中国环境科学, 2019,39(6):2560-2567. Xu H, Chen D, Chen J, et al. Effects of nitrogen and phosphorus forms and concentrations on the growth of Microcystis aeruginosa and Scenedesmus obliquus[J]. China Environmental Science, 2019,39(6):2560-2567.
[15]
李迷.螺旋藻保种技术的研究[D]. 天津:天津商业大学, 2015.
[16]
Chen W, Zhang C, Song L, et al. A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae[J]. Journal of Microbiological Methods, 2015,77(1):41-7.
[17]
Andersen J E T. The standard addition method revisited[J]. Trac Trends in Analytical Chemistry, 2017,89(Complete):21-33.
[18]
Bligh E G, Dyer W J. A rapid method of total lipid extraction and purification[J]. Can J BiochemPhysiol, 1959,37(8):911.
[19]
Grintzalis K, Georgiou C D, Schneider Y J. An accurate and sensitive Coomassie Brilliant Blue G-250-based assay for protein determination[J]. Analytical Biochemistry, 2015,480:28-30.
[20]
Pirra R J. The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b[J]. Photosynthesis Research, 2002,73(1-3):149-56.
[21]
简建波,邹定辉,刘文华,等.三角褐指藻对铜离子长期暴露的生理响应[J]. 海洋通报, 2010,29(1):65-71. Jian J B, Zou D H, Liu W H, et al. Physiological responses of Phaeodactylumtriconutum to long-term copper (Ⅱ) exposure[J]. Marine Science Bulletin, 2010,29(1):65-71.
[22]
Eyssautier C S, Vaillant G N, Gommeaux M, et al. Efficacy of different chemical mixtures against green algal growth on limestone:A case study with Chlorella vulgaris[J]. International Biodeterioration& Biodegradation, 2015,103:59-68.
[23]
饶本强,张列宇,吴沛沛,等.集球藻对盐胁迫的生理适应及细胞结构变化[J]. 水生生物学报, 2012,36(2):329-38. Rao B Q, Zhang L Y, Wu P P, et al. Changes on cellular structures and physiological adaptation of Palmellococcus sp..Subjected to Salt Stress[J]. Acta Hydrobiologica Sinica, 2012,36(2):329-38.
[24]
Pancha I, Chokshi K, Maurya R, et al. Salinity induced oxidative stress enhanced biofuel production potential of microalgae Scenedesmus sp. CCNM 1077[J]. Bioresource Technology, 2015,189:341-8.
[25]
董正臻,董振芳,丁德文.快速测定藻类生物量的方法探讨[J]. 海洋科学, 2004,28(11):1-2. Dong Z Z, Dong Z F, Ding D W. A method of quick determination of algal biomass[J]. Marine Sciences, 2004,28(11):1-2.
[26]
Sairam R K, Rao V, Srivastava, et al. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration[J]. Plant Science, 2002,163(5):1037-46.
[27]
Avron, M. The osmotic components of halotolerant algae[J]. Trends in Biochemical Sciences, 1986,11(1):5-6.
[28]
Sadka A, Himmelhoch S, Zamir A. A 150kilodalton cell surface protein is induced by salt in the halotolerant green alga Dunaliellasalina[J]. Plant Physiology, 1991,95(3):822-31.
[29]
Alvensleben N V, Magnusson M, Heimann K. Salinity tolerance of four freshwater microalgal species and the effects of salinity and nutrient limitation on biochemical profiles[J]. Journal of Applied Phycology, 2016,28(2):861-76.
[30]
Galinski E A, Hans G. Microbial behaviour in salt-stressed ecosystems[J]. Fems Microbiology Reviews, 1994,15(2/3):95-108.
[31]
李战,冯奇俊,童昱,等.铜绿紫球藻(Porphyridiumaerugineum) 755培养的研究[J]. 植物研究, 2004,24(3):317-22. Li Z, Feng Q J, Tong Y, et al. Studies on the culture of Porphyridiumaerugineum 755[J]. Bulletin of Botanical Research, 2004,24(3):317-22.
[32]
Karuppanapandian, Thirupathi,M, Jun C, Kim, et al. Reactive oxygen species in plants:their generation, signal transduction, and scavenging mechanisms[J]. Australian Journal of Crop Science, 2011,5(6):709-725.
[33]
Nikolaou A, Bernardi A, Meneghesso A, et al. A model of chlorophyll fluorescence in microalgae integrating photoproduction, photoinhibition and photoregulation[J]. Journal of Biotechnology, 2015,194(5):91-9.
[34]
梁英,冯力霞,田传远,等.盐胁迫对塔胞藻生长及叶绿素荧光动力学的影响[J]. 中国海洋大学学报(自然科学版), 2006,(5):726-732. Liang Y, Feng L X, Tian C Y, et al. Effects of Salt Stress on the Growth and Chlorophyll Fluorescence of Pyramidomonas sp.[J]. Periodical of Ocean University of China, 2006,(5):726-732.