The potential mechanisms for anaerobic phenol degradation and methanogenesis promotion by biochar addition were evaluated by batch experiments,and the effects of biochar derived at 300,500 and 700℃ on lag time,maximum methane production rate and microbial community structure were elucidated in this study. The results indicated a strong linear relationship between the electron exchange capacity of biochar and Rmax (R2=0.997). Furthermore, methanogenic lag time was notably shortened from 15.0days to 1.1~3.2days, and the maximum CH4 production rate was increased from 4.0mL/d to 10.4~13.9mL/d by biochar addition (15g/L). Moreover, the methanogenesis of phenol was optimized by addition of biochar prepared at 500℃ because of the most abundance of electrochemically active quinones.In addition, microbial community analysis showed that the electroactive Geobacter and Methanosaeta microbial consortia were enriched by biochar addition. It was further demonstrated that biochar addition fueled the methane production rate of phenol by promoting interspecies electron transfer.
高新, 王高骏, 李倩, 陈荣. 生物炭强化苯酚厌氧降解产甲烷特性[J]. 中国环境科学, 2020, 40(2): 631-639.
GAO Xin, WANG Gao-jun, LI Qian, CHEN Rong. Characteristics of enhanced anaerobic degradation and methanogenesis of phenol by biochar addition. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(2): 631-639.
Aktas Ö, Çeçen F. Adsorption reversibility and bioregeneration of activated carbon in the treatment of phenol[J]. Water Science and Technology, 2007,55(10):237-244.
El Naas M H, Al Zuhair S, Alhaija M A. Removal of phenol from petroleum refinery wastewater through adsorption on date~pit activated carbon[J]. Chemical Engineering Journal, 2010,162(3):997-1005.
Sable S S, Shah K J, Chiang P C, et al. Catalytic oxidative degradation of phenol using iron oxide promoted sulfonated ~ZrO2 by Advanced Oxidation Processes (AOPs)[J]. Journal of the Taiwan Institute of Chemical Engineers, 2018,91:434-440.
Hussain A, Dubey S K, Kumar V. Kinetic study for aerobic treatment of phenolic wastewater[J]. Water Resources and Industry, 2015,11:81-90.
Mao C, Feng Y, Wang X, et al. Review on research achievements of biogas from anaerobic digestion[J]. Renewable and Sustainable Energy Reviews, 2015,45:540-555.
Fang H H, Liang D W, Zhang T, et al. Anaerobic treatment of phenol in wastewater under thermophilic condition[J]. Water Research, 2006, 40(3):427-434.
Keith C L, Bridges R L, Fina L R, et al. The anaerobic decomposition of benzoic acid during methane fermentation[J]. Archives of Microbiology, 1978,118(2):173-176.
Bakker G. Anaerobic degradation of aromatic compounds in the presence of nitrate[J]. FEMS Microbiology Letters, 1977,1(2):103-107.
Rotaru A E, Shrestha P M, Liu F, et al. A new model for electron flow during anaerobic digestion:direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane[J]. Energy & Environmental Science, 2014,7(1):408-415.
Yuan Y, Bolan N, Prevoteau A, et al. Applications of biochar in redox mediated reactions[J]. Bioresource Technology, 2017,246:271-281.
Poirier S, Dejean S, Chapleur O. Support media can steer methanogenesis in the presence of phenol through biotic and abiotic effects[J]. Water Research, 2018,140:24-33.
Prado A, Berenguer R, Esteve Núñez A. Electroactive biochar outperforms highly conductive carbon materials for biodegrading pollutants by enhancing microbial extracellular electron transfer[J]. Carbon, 2019,146:597-609.
宇文超岁,李倩,陈荣,等.生物炭对严重酸积累的甲烷发酵系统快速恢复的促进效果[J]. 环境工程, 2018,36(12):155-159. Yu W C, Li Q, Chen R, et al. Promotive effect of biochar on rapid recovery of methane fermentation system from severe acid accumulation[J]. Environmental Engineering, 2018,36(12):155-159.
郭克俭,何品晶,邵立明,等.应用生物炭改善填埋场渗滤液的厌氧消化性能[J]. 中国环境科学, 2018,38(5):1801-1810. Guo K J, He P J, Shao L M, et al. Application of biochar to improve anaerobic degradation of landfill leachate[J]. China Environmental Science, 2018,36(12):155-159.
Yuan H Y, Ding L J, Zama E F, et al. Biochar Modulates Methanogenesis through Electron Syntrophy of Microorganisms with Ethanol as a Substrate[J]. Environmental Science & Technology, 2018, 52(21):12198-12207.
Peng Z, Shiling Z, Jia L, et al. Surface properties of activated sludge-derived biochar determine the facilitating effects on Geobacter co-cultures[J]. Water Research, 2018,142:441-451.
程扬,沈启斌,刘子丹,等.两种生物炭的制备及其对水溶液中四环素去除的影响因素[J]. 环境科学, 2019,40(3):1328-1336. Cheng Y, Shen Q B, Liu Z D, et al. Preparation of two kinds of biochar and the factors influencing tetracycline removal from aqueous solution[J]. Environmental Science, 2019,40(3):1328-1336.
马锋锋,赵保卫,刁静茹,等.磁性生物炭对水体中对硝基苯酚的吸附特性[J]. 中国环境科学, 2019,39(1):172-180. Ma F F, Zhao B W, Diao J R, et al. Adsorption characteristics of p-nitrophenol removal by magnetic biochar[J]. China Environmental Science, 2019,39(1):170-178.
Gabhi R S, Kirk D W, Jia C Q. Preliminary investigation of electrical conductivity of monolithic biochar[J]. Carbon, 2017,116:435-442.
Qiu Y L, Hanada S, Ohashi A, et al. Syntrophorhabdus aromaticivorans gen. nov. sp. nov. the first cultured anaerobe capable of degrading phenol to acetate in obligate syntrophic associations with a hydrogenotrophic methanogen[J]. Applied and Environmental Microbiology, 2008,74(7):2051-2058.
Klüpfel L, Keiluweit M, Kleber M, et al. Redox Properties of Plant Biomass~Derived Black Carbon (Biochar)[J]. Environmental Science & Technology, 2014,48(10):5601-5611.
Yan W, Sun F, Liu J, et al. Enhanced anaerobic phenol degradation by conductive materials via EPS and microbial community alteration[J]. Chemical Engineering Journal, 2018,352:1-9.
Poirier S, Bize A, Bureau C, et al. Community shifts within anaerobic digestion microbiota facing phenol inhibition:Towards early warning microbial indicators?[J]. Water Research, 2016,100:296-305.
Summers Z M, Fogarty H E, Leang C, et al. Direct exchange of electrons within aggregates of an evolved syntrophic coculture of anaerobic bacteria[J]. Science, 2010,330(6009):1413-1415.
Zhao Z, Zhang Y, Holmes D E, et al. Potential enhancement of direct interspecies electron transfer for syntrophic metabolism of propionate and butyrate with biochar in up~flow anaerobic sludge blanket reactors[J]. Bioresource Technology, 2016,209:148-156.