Effects of temperature changes at high altitude on phosphorus removal and related metabolic pathways in activated sludge
WANG Jun1, GUO Ming-zhe1, YOU Jun-hao1, ZONG Yong-chen1, FU Chun-hui1, ZHANG Dong-yan1,2
1. Water Conservancy Project & Civil Engineering College, Tibet Agriculture & Animal Husbandry University, Linzhi 860000, China; 2. State Key Laboratory of Coal Combustion, Huazhong University of Science and Technology, Wuhan 430074, China
Abstract：The A2O process in highland habitats was used in this paper to investigate the phosphorus removal performance of anaerobic, anoxic and aerobic reactors under different temperature conditions, including the response of dominant polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) and related metabolic pathways to temperature changes. The results show that the best phosphorus removal performance (82.99%~88.39%) was achieved at 20℃, but lower than that of the same process in the plains; and GAOs did not have competitive advantage and did not increase with an increase in temperature. The relative abundance of Acinetobacter (PAO), Dechloromonas and Hydrogenophaga (denitrifying PAOs) was higher in the anoxic reactor under the environmental conditions of sludge swelling at 10℃ and unfavorable growth of PAOs at 25℃, which could be attributed to the improvement of phosphorus removal in the anoxic reactor. In addition, glycolysis (EMP) was identified as the main pathway of glucose metabolism. Evidently, the main synthetic pathway of polyhydroxyvalerate (PHV) was Propionyl-CoA; and the ATP required for both anaerobic and aerobic reactors came mainly from the tricarboxylic acid cycle (TCA cycle) pathway.
王俊, 郭明哲, 尤俊豪, 宗永臣, 傅椿惠, 张东艳. 高海拔温度变化对活性污泥中磷去除及相关代谢途径的影响[J]. 中国环境科学, 2023, 43(5): 2364-2372.
WANG Jun, GUO Ming-zhe, YOU Jun-hao, ZONG Yong-chen, FU Chun-hui, ZHANG Dong-yan. Effects of temperature changes at high altitude on phosphorus removal and related metabolic pathways in activated sludge. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(5): 2364-2372.
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