Abundance of aerobic ammonia-oxidizing and nirS type denitrifying microorganisms in sediments of Lancang River
WU Zi-xian1,2, CHEN Hao-dong1,2, LI Zhu1,2,3, CHEN Zhuo4, LIAO Ming-jun1,2,3
1. School of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan 430068, China; 2. Hubei Key Laboratory of Ecological Remediation for Rivers-Lakes and Algal Utilization, Wuhan 430068, China; 3. Cooperative Innovation Center of Industrial Fermentation, Ministry of Education & Hubei Province, Wuhan 430068, China; 4. Wuhan Huanjing Detecion Service Co. Lto., Wuhan 430048, China
Abstract：In order to explore the impact of cascade reservoir on nitrogen cycling microorganisms in the Lancang River , the abundance of ammonia oxidizing bacteria (AOB), ammonia oxidizing archaea (AOA) and nirS type denitrifying microorganisms in the river sediments were studied. Sediment samples were collected from 19 sites from Yanjing to Ganlanba in Yunnan Province. The abundance of AOB-amoA, AOA-amoA and nirS genes were quantified through qPCR. The physical and chemical factors of sediments, pore water, and water bodies were determined at the mean time. The results showed that AOB-amoA gene abundance ranged from 0.82x107 to 4.25x107 copies/g, AOA-amoA gene abundance ranged from 0.83x107 to 6.87x107 copies/g, and the nirS gene abundance ranged from 0.72x107 to 7.32x107 copies/g; the ratio of AOA/AOB is between 0.35 and 2.17, and AOA is not superior to AOB in the sediment. The abundance of AOA-amoA, AOB-amoA and nirS showed no significant difference in the river, reservoir and tributaries sites, indicating that the dam construction has no significant impact on the spatial distribution of nitrogen cycling microorganism abundances. Ammonia, total phosphorus and total nitrogen concentration in pore water are the main environmental factors affecting AOA abundance. AOB abundance is mainly affected by water temperature, total phosphorus, total nitrogen and total organic carbon content in sediments. The abundance of nirS type denitrifying microorganisms is mainly affected by total nitrogen concentration in pore water, and organic carbon content in sediment.
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