Effects of grassland utilization on soil nematode community structure and metabolic footprint
WU Zhen-dan1, LU Jun-yan1, MA Shang-fei3, YE He1, ZHANG Ya-ling1, YANG Dian-lin4, HONG Mei1,2
1. College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010018, China; 2. Key Laboratory of Agricultural Ecological Security and Green Development at Universities of Inner Mongolia Autonomous Region, Inner Mongolia Key Laboratory of Soil Quality and Nutrient Resources, Hohhot 010018, China; 3. Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, China; 4. Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Abstract：In August 2021, soil nematodes were collected from mowed, free-grazed, and fenced (control) plots of Stipa baicalensis meadow grassland to investigate the effects of different grassland utilization on the structural characteristics and metabolic footprints of soil nematode communities. A total of 15632 soil nematodes belonging to 55 genera were captured in this study, and the dominant genera were Cervidellus, Tylencholaimus, and Filenchus, which accounted for 7.05%, 7.14%, and 6.18% of the total captures, respectively. The total nematode abundance was significantly lower in free-grazing habitats compared to fenced habitats. In terms of functional groups, the relative abundance of phytophagous nematodes (33.84% ~ 37.45%) and c-p 2groups (38.78% ~ 47.58%) was the largest in all three habitats, and both of them increased significantly in mowed and free-grazing habitats. Concerning diversity and ecological indices, the Maturity index (MI) and Enrichment index (EI) of free-grazing habitats decreased significantly, and the basal index (BI) increased significantly. This indicates that the diversity and stability of nematode communities decreased in free-grazing habitats, and the food web increased in resistance to disturbance. The Structural index (SI) of mowing habitats increased significantly, indicating that the connectivity of food webs in mowing habitats increased, and the food chain was lengthened. The Nematode channel ratio (NCR) is >0.5and the Wasilewska index (WI) is >1under different grassland utilization modes, indicating that the microbial nematodes were the main bearers of the soil mineralization pathway in the meadow grassland, and the bacterial pathway dominates the decomposition of organic matter. Concerning metabolic footprints, both mowing and free-grazing habitats decreased the compound metabolic footprint (TNf) and functional metabolic footprint of nematodes, and the food webs were in a structured state. In contrast, fenced habitats significantly increased the overall activity and carbon flow of nematode communities, and the food webs were in a more stable metabolic equilibrium state. The below-ground biomass (BGB), the number of plant individuals (P-N), and available potassium (AK) were the main environmental factors affecting the development of nematode communities under different grassland utilization. In conclusion, the soil nematode community structure varied significantly among different grassland utilization modes, and the changes of environmental factors in different habitats were the main factors driving the changes of soil nematode community.
武振丹, 卢俊艳, 马尚飞, 叶贺, 张雅玲, 杨殿林, 红梅. 草地利用方式对土壤线虫群落结构及代谢足迹的影响[J]. 中国环境科学, 2024, 44(6): 3342-3352.
WU Zhen-dan, LU Jun-yan, MA Shang-fei, YE He, ZHANG Ya-ling, YANG Dian-lin, HONG Mei. Effects of grassland utilization on soil nematode community structure and metabolic footprint. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(6): 3342-3352.
[1] David R K, Han G D, Hou X Y, et al. Innovative grassland management systems for environmental and livelihood benefits[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013,110(21):8369-8374. [2] Li X, Lin G, Jiang D, et al. Spatiotemporal evolution characteristics and the climatic response of carbon sources and sinks in the Chinese grassland ecosystem from 2010 to 2020[J]. Sustainability, 2022,14(14):8461-8461. [3] Gang C C, Zhou W, Chen Y Z, et al. Quantitative assessment of the contributions of climate change and human activities on global grassland degradation[J]. Environmental Earth Sciences, 2014,72(11):4273-4282. [4] 薛会英,罗大庆,胡锋,等.短期围封对西藏北部高寒草甸土壤线虫群落的影响[J].生态学报, 2016,36(19):6139-6148. Xue H Y, Luo D Q, Hu F, et al. Effect of short-term enclosure on soil nematode communities in an alpine meadow in Northern Tibet[J]. Acta Ecologica Sinica, 2016,36(19):6139-6148. [5] Chen T, Tang G, Yuan Y, et al. Unraveling the relative impacts of climate change and human activities on grassland productivity in Central Asia over last three decades[J]. Science of Total Environments, 2020,743:140649. [6] 张景慧,王铮,黄咏梅,等.草地利用方式对温性典型草原优势种植物功能性状的影响[J].植物生态学报, 2021,45(7):818-833. Zhang J H, Wang Z, Huang Y M, et al. Effects of grassland utilization on the functional traits of dominant plants in a temperate typical steppe[J]. Chinese Journal of Plant Ecology, 2021,45(7):818-833. [7] 张超,闫瑞瑞,梁庆伟,等.不同利用方式下草地土壤理化性质及碳、氮固持研究[J].草业学报, 2021,30(4):90-98. Zhang C, Yan D D, Liang Q W, et al. Study on soil physical and chemical properties and carbon and nitrogen sequestration of grassland under different utilization modes[J]. Acta Prataculturae Sinice, 2021, 30(4):90-98. [8] Johan H D V, Stefan G, Devin R, et al. Soil nematode abundance and functional group composition at a global scale[J]. Nature, 2019, 572(7768):194-198. [9] Yeates G W. Nematodes as soil indicators:functional and biodiversity aspects[J]. Biology and Fertility of Soils, 2003,37:199-210. [10] Ruan W B, Sang Y, Chen Q, et al. The response of soil nematode community to nitrogen, water, and grazing history in the Inner Mongolian steppe, China[J]. Ecosystems, 2021,15(7):1121-1133. [11] Ferris H, Bongers T, De Goede R G, et al. A framework for soil food web diagnostics:extension of the nematode faunal analysis concept[J]. Applied Soil Ecology, 2001,18(1):13-29. [12] Pan F, Yan R, Zhao J, et al. Effects of grazing intensity on soil nematode community structure and function in different soil layers in a Meadow Steppe[J]. Plant and Soil, 2021,471(1):33-46. [13] 荆佳强,萨仁其力莫格,秦洁,等.不同利用方式对贝加尔针茅草原土壤活性有机碳的影响[J].草业学报, 2022,31(1):47-56. Jing J Q, Sa R Q L M G, Qin J, et al. Effects of different land-use patterns on soil active organic carbon in Stipa baicalensis steppe in Inner Mongolia[J]. Acta Prataculturae Sinica, 2022,31(1):47-56. [14] Wang X T, Nielsen U N, Yang X L, et al. Grazing induces direct and indirect shrub effects on soil nematode communities[J]. Soil Biology&Biochemistry, 2018,121:193-201. [15] Hu J, Wu J H, Ma M J, et al. Nematode communities response to long-term grazing disturbance on Tibetan plateau[J]. European Journal of Soil Biology, 2015,69:24-32. [16] 武振丹,红梅,马尚飞,等.长期养分添加对贝加尔针茅草原中小型土壤节肢动物群落的影响[J].应用与环境生物学报, 2022,28(6):1534-1541. Wu Z D, Hong M, Ma S F, et al. Effects of long-term nutrient addition on meso-micro soil arthropod communities in a Stipa baicalensis grassland[J]. Chinese Journal of Applied and Environmental, 2022, 28(6):1534-1541. [17] 尹文英.中国土壤动物检索图鉴[M].北京:科学出版社, 1998:51-89. Yin W Y. Pictorial keys to soil animals of China[M]. Beijing:Science Press, 1998:51-89. [18] Bardgett R D, Usher M B, Hopkins D W, et al. Biological diversity and function in soils[M]. Cambridge:Cambridge University Press, 2005:117-256. [19] Yeates G W, Bongers T, De Goede R G M, et al. Feeding habits in soil nematode families and genera-an outline for soil ecologists[J]. Journal of Nematology, 1993,25(3):315-331. [20] 鲍士旦.土壤农化分析[M].第3版.北京:中国农业出版社, 2000:189-196. Bao S D. Soil and agricultural chemistry analysis (3rd ed.)[M]. Beijing:China Agriculture Press, 2000:189-196. [21] Ferris H. Form and function:metabolic footprints of nematodes in the soil food web[J]. European Journal of Soil Biology, 2010,46(2):97-104. [22] Cesarz S, Ciobanu M, Wright A J, et al. Plant species richness sustains higher trophic levels of soil nematode communities after consecutive environmental perturbations[J]. Oecologia, 2017,184(3):715-728. [23] Franco A L C, Gherardi L A, Tomasel C M, et al. Drought suppresses soil predators and promotes root herbivores in mesic, but not in xeric grasslands[J]. Proceedings of the National Academy of Sciences of the United States of America, 2019,116(26):12883-12888. [24] Yang J, Wu X, Chen Y, et al. Combined attributes of soil nematode communities as indicators of grassland degradation[J]. Ecological Indicators, 2021,131:108215. [25] Martinez J G, Torres M A, Dos Santos G, et al. Influence of heavy metals on nematode community structure in deteriorated soil by gold mining activities in Sibutad, southern Philippines[J]. Ecological Indicators, 91(1):712-721. [26] Shao C, Chen J, Li L, et al. Ecosystem responses to mowing manipulations in an arid Inner Mongolia steppe:An energy perspective[J]. Journal of Arid Environments, 2012,82:1-10. [27] Zhao J, Chen J, Wu H, et al. Effects of mowing frequency on soil nematode diversity and community structure in a Chinese meadow steppe[J]. Sustainability, 2021,13(10):5555. [28] 李学斌,陈林,樊瑞霞,等.围封条件下荒漠草原4种典型植物群落枯落物输入对土壤理化性质的影响[J].浙江大学学报(农业与生命科学版), 2015,41(1):101-110. Li X B, Chen L, Fan R X, et al. Effect of four typical piant community litter input on soil physical properties under the fenced condition in desert steppe[J]. Journal of Zhejiang University (Agriculture and Life Science), 2015,41(1):101-110. [29] 卢俊艳.草地利用方式对贝加尔针茅草原土壤线虫群落特征的影响[D].呼和浩特:内蒙古农业大学, 2022. Lu J Y. Effects of grassland utilization modes on soil nematode community characteristics in Baikal Stipa Steppe[D]. Hohhot:Inner Mongolia Agricultural University, 2022. [30] 张宇晨,红梅,赵巴音那木拉,等.荒漠草原土壤线虫对氮沉降及降雨变化的响应[J].中国环境科学, 2021,41(6):2788-2797. Zhang Y C, Hong M, Zhao B Y N M L, et al. Response of soil nematodes in desert steppe to nitrogen deposition and rainfall changes[J]. China Environmental Science, 2021,41(6):2788-2797. [31] Chen Y L, Hu H W, Han H Y, et al.Abundance and community structure of ammonia-oxidizing Archaea and Bacteria in response to fertilization and mowing in a temperate steppe in Inner Mongolia[J]. FEMS Microbiology Ecology, 2014,89(1):67-79. [32] Zhang X K, Guan P T, Wang Y L, et al. Community composition, diversity and metabolic footprints of soil nematodes in differently-aged temperate forests[J]. Soil Biology&Biochemistry, 2015,80:118-126. [33] 程云云,孙涛,王清奎,等.模拟氮沉降对温带森林土壤线虫群落组成和代谢足迹的影响[J].生态学报, 2018,38(2):475-848. Cheng Y Y, Sun T, Wang Q K, et al. Effects of simulated nitrogen deposition on temperate forest soil nematode communities and their metabolic footprints[J]. Acta Ecologica Sinica, 2018,38(2):475-484. [34] 胡向敏,乌仁其其格,刘琼,等.不同利用方式下贝加尔针茅草甸草原群落多样性变化[J].干旱区资源与环境, 2021,35(4):189-194. Hu X M, Wu R Q Q G, Liu Q, et al. Species diversity changes in Stipa baicalensis meadow steppe under different utilizing types[J]. Journal of Arid Land Resources and Environment, 2021,35(4):189-194. [35] 薛会英,罗大庆,王鸿源,等.藏北高寒草甸土壤线虫群落对围封及自由放牧的响应[J].土壤学报, 2017,54(2):480-492. Xue H Y, Lou D Q, Wang H Y, et al. Effects of free grazing or enclosure on soil nematodes in alpine meadows in North Tibet, China[J]. Acta Pedologica Sinica, 2017,54(2):480-492. [36] Wilschut R A, Geisen S. Nematodes as drivers of plant performance in natural systems[J]. Trends in Plant Science, 2021,26(3):237-247. [37] Du X F, Li Y B, Han X, et al. Using high-throughput sequencing quantitatively to investigate soil nematode community composition in a steppe-forest ecotone[J]. Applied Soil Ecology, 2020,152:103562. [38] Zhang M, Lui Y L, Wei Q Q, et al. Biochar application ameliorated the nutrient content and fungal community structure in different yellow soil depths in the karst area of southwest China[J]. Frontiers in Plant Science, 2022,13:1020832. [39] 郭衍银,王秀峰,徐坤,等.姜田土壤特性对南方根结线虫密度的影响[J].农业机械学报, 2004,35(6):202-205,223. Guo Y Y, Wang X F, Xu K, et al. Effect of soil characteristic on density of south root-knot nematodes, meloidogyne incognita, in Ginger Field[J]. Transactions of the Chinese Society for Agricultural Machinery, 2004,35(6):202-205,223.
