餐厨垃圾干式厌氧消化污泥膨胀微生态特征

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Abstract A plug-flow reactor (PFR)(R1) and a completely-stirred tank reactor (CSTR)(R2) were operated under mesophilic temperature (37±1℃) for high-solids digestion of kitchen waste to investigate the microbial community characteristics before and after sludge-bulking using MiSeq high-throughput sequencing technology.The results showed that the archaeal community structure changed little after sludge-bulking,and acetoclastic methanogen Methanosaeta and mixotrophic methanogen Methanosarcina dominated in both reactors.There was a marked increase in the relative abundance of bacteria genera that might be related to sludge bulking.Those proliferated bacteria genera are those capable of producing biosurfactants (Corynebacterium,Lactobacillus,etc.) and those containing mycolic acids in cell walls (Actinomyces,Corynebacterium,etc.).In addition,volatile fatty acids (VFAs) and total ammonia nitrogen (TAN) were accumulated in these reactors before sludge-bulking.Accordingly,bacteria that can contribute to the accumulation of VFAs (Petrimonas,Anaerosalibacter and Fastidiosipila,etc.) and TAN (Proteiniphilum,Tepidimicrobium and Aminobacterium,etc.) were observed to proliferate.

Key words： food waste high-solid anaerobic digestion sludge bulking microorganisms MiSeq

Received: 12 August 2017

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	HE Qin
	LI Lei
	QU Li
	ZHAO Xiao-fei
	WU Di
	PENG Xu-ya

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HE Qin,LI Lei,QU Li等. Microbial characteristics of bulking sludge in high-solids anaerobic digestion of kitchen waste[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(3): 1010-1017.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2018/V38/I3/1010

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