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Co-digestion characteristics of vegetable straws and livestock manure and analysis of key microorganisms |
MENG Yan, LI Yi, DU Zhong-ping, HAN Rui |
Qinghai Key Laboratory of Vegetable Genetics and Physiology, Academy of Agriculture and Forestry Sciences, Qinghai University, Qinghai Xining 810016, China |
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Abstract The characteristics of methane yields and the changes of key microorganisms in the co-digestion of cucumber straw, tomato straw, eggplant straw and pepper straw with pig manure, cow manure and sheep manure were investigated by batch fermentation. The co-digestion of vegetable straws with livestock manure had a synergistic effect, and the methanogenic effect of the four vegetable straws mixed with pig manure was the best, followed by cow manure and sheep manure. Among them, cucumber straw and eggplant straw with pig manure at a ratio of 1:2 had the maximum cumulative methane yields of 338.28mL/g VS and 283.00mL/g VS respectively, which increased by 65.15% and 67.36% compared to the single digestion of the respective straws. The maximum methane yields for tomato straw and pepper straw with pig manure were 317.90mL/g VS and 251.17mL/g VS at a ratio of 1:4, which were 69.07% and 92.93% higher than the single digestion of the tomato and pepper straws respectively. The microbial analysis showed that the relative abundance of Syner-01 was higher in the samples added with pig manure and in all co-digested samples. The methanogenic archaea taxa in the co-digested samples added with pig manure were more abundant, with higher relative abundance of Methanocellus and Methanoplanus, while the abundance of Methanocorpusculum were higher after adding cow manure, indicating that the addition of different manures to vegetable straws for co-digestion could lead to the enrichment of hydrotropic methanogenic archaea.
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Received: 13 January 2023
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