17α-乙炔雌二醇胁迫下零价铁对硝化污泥中抗性基因的影响

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Abstract The prevalence of antibiotic resistance genes (ARGs) has been widely concerned by the worldwide researchers. However, the effect of zero valent iron (ZVI) on ARGs in nitrifying sludge has been rarely studied. A nitrifying sludge sequencing batch reactor was used to remove 17α-ethinylestradiol (EE2). The effect of ZVI on ARGs in the process of EE2 removal by nitrifying sludge was investigated based on metagenome sequencing, and the potential mechanism was analyzed by combining with the structure of bacterial community. The results showed that the abundance of ARGs increased by 724.42TPM (transcripts per million) during the removal of EE2, and ZVI reduced the increasing trend of ARGs abundance. EE2 and ZVI changed the distribution of ARGs subtypes, but had no significant effect on the types and subtypes amount of ARGs. The abundance of ARGs belonging to high-risk ranks (Q1 and Q2) increased by 555.75, 151.08TPM in the treatment of EE2, but ZVI reduced the resistance risk. Multidrug was the most abundant high-risk (Q1 and Q2 rank) ARGs in nitrifying sludge, accounting for 49.23%. Microbial community was an important driving factor for ARGs in nitrifying sludge. Some bacteria, such as Sphingopyxis, were significantly associated with multiple ARGs positively, and might be the common potential hosts of multiple ARGs. This study provides a scientific basis and theoretical guidance for ZVI to reduce ARGs and resistance risks in the treatment of emerging pharmaceutical contaminants by biological nitrogen removal process.

Key words： nitrifying sludge 17α-ethinylestradiol (EE2) antibiotic resistance genes (ARGs) zero valent iron (ZVI)

Received: 17 May 2023

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X703

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	WANG Li-li
	LI An-jie
	ZHANG Chuan-guo

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WANG Li-li,LI An-jie,ZHANG Chuan-guo. Effect of zero valent iron on antibiotic resistance gene in nitrifying sludge under 17α-ethinylestradiol stress[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(1): 548-554.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I1/548

[1]	Tang Z, Zhang Y, Zhang S, et al.Temporal dynamics of antibiotic resistant bacteria and antibiotic resistance genes in activated sludge upon exposure to starvation [J].Science of the Total Environment, 2022,840:156594.
[2]	Wang L, Liu Z, Jiang X, et al.Aerobic granulation of nitrifying activated sludge enhanced removal of 17alpha-ethinylestradiol[J].Science of the Total Environment, 2021,801:149546.
[3]	Wang B, Ni B, Yuan Z, et al.Unravelling kinetic and microbial responses of enriched nitrifying sludge under long-term exposure of cephalexin and sulfadiazine [J].Water Research, 2020,173:115592.
[4]	Wang Z, Gao J, Wang S, et al.Triclocarban shifted the microbial communities and promoted the spread of antibiotic resistance genes in nitrifying granular sludge system [J].Bioresource Technology, 2022, 347:126429.
[5]	Yan R, Wang Y, Li J, et al.Determination of the lower limits of antibiotic biodegradation and the fate of antibiotic resistant genes in activated sludge:Both nitrifying bacteria and heterotrophic bacteria matter [J].Journal of Hazardous Materials, 2022,425:127764.
[6]	Shi X, Wei W, Wu L, et al.Zero-valent iron mediated biological wastewater and sludge treatment [J].Chemical Engineering Journal, 2021,426:130821.
[7]	Wang L, Li A.Impact of zero-valent iron on nitrifying granular sludge for 17alpha-ethinylestradiol removal and its mechanism [J].Chemosphere, 2023,333:138904.
[8]	Li W, Pang L, Chatzisymeon E, et al.Effects of micron-scale zero valent iron on behaviors of antibiotic resistance genes and pathogens in thermophilic anaerobic digestion of waste activated sludge [J].Bioresource Technology, 2023,376:128895.
[9]	Zhang Z, Zhang Q, Wang T, et al.Assessment of global health risk of antibiotic resistance genes [J].Nature Communication, 2022,13(1):1553.
[10]	Bondarczuk K, Markowicz A, Piotrowska-Seget Z.The urgent need for risk assessment on the antibiotic resistance spread via sewage sludge land application [J].Environment International, 2016,87:49-55.
[11]	Xu Y, Yang S, You G, et al.Attenuation effects of iron on dissemination of antibiotic resistance genes in anaerobic bioreactor:Evolution of quorum sensing, quorum quenching and dynamics of community composition [J].Journal of Hazardous Materials, 2021,416:126136.
[12]	Yang H, Deng L, Xiao Y, et al.Construction of autotrophic nitrogen removal system based on zero-valent iron (ZVI):Performance and mechanism [J].Water Science and Technology, 2020,82(12):2990-3002.
[13]	Huang H, Chen Y, Yang S, et al.CuO and ZnO nanoparticles drive the propagation of antibiotic resistance genes during sludge anaerobic digestion:possible role of stimulated signal transduction [J].Environmental Science:Nano, 2019,6(2):528-539.
[14]	Luo J, Zhang L, Du W, et al.Metagenomic approach reveals the fates and mechanisms of antibiotic resistance genes exposed to allicins during waste activated sludge fermentation:Insight of the microbial community, cellular status and gene regulation [J].Bioresource Technology, 2021,342:125998.
[15]	Qiu X, Zhou G, Wang H.Nanoscale zero-valent iron inhibits the horizontal gene transfer of antibiotic resistance genes in chicken manure compost [J].Journal of Hazardous Materials, 2022,422:126883.
[16]	Ma J, Gu J, Wang X, et al.Effects of nano-zerovalent iron on antibiotic resistance genes during the anaerobic digestion of cattle manure [J].Bioresource Technology, 2019,289:121688.
[17]	邢月,程艳,程刚,等.基于宏基因组学方法探究西北内陆河抗生素抗性基因赋存特征及其影响因素[J].中国环境科学, 2023, 43(6):3077-3086.Xing Y, Zhang W, Cheng Y, et al.Distribution characteristics and influencing factors of antibiotic resistance genes in inland rivers of Northwest China [J].China Environmental Science, 2023,43(6):3077-3086.
[18]	Ryan M P, Sevjahova L, Gorman R, et al.The emergence of the genus comamonas as important opportunistic pathogens:Pathogens [Z].2022,11(9):1032.
[19]	Guo J H, Li J, Chen H, et al.Metagenomic analysis reveals wastewater treatment plants as hotspots of antibiotic resistance genes and mobile genetic elements [J].Water Research, 2017,123:468-478.
[20]	Saunders A M, Albertsen M, Vollertsen J, et al.The activated sludge ecosystem contains a core community of abundant organisms [J].The Isme Journal, 2016,10(1):11-20.
[21]	Roy A, Dutta A, Pal S, et al.Biostimulation and bioaugmentation of native microbial community accelerated bioremediation of oil refinery sludge [J].Bioresource Technology, 2018,253:22-32.
[22]	Gao D, Liu L, Liang H, et al.Aerobic granular sludge:characterization, mechanism of granulation and application to wastewater treatment [J].Critical Reviews in Biotechnology, 2011,31(2):137-152.
[23]	Korry B J, Belenky P.Trophic level and proteobacteria abundance drive antibiotic resistance levels in fish from coastal New England [J].Animal Microbiome, 2023,5(1):16.
[24]	徐秋鸿,刘曙光,娄厦,等.长江口近岸地区抗生素抗性基因与微生物群落分布特征[J].环境科学, 2023,44(1):158-168.Xu Q H, Liu S G, Lou X, et al.Distributions of Antibiotic Resistance Genes and Microbial Communities in the Nearshore Area of the Yangtze River Estuary [J].Environmental Science, 2023,44(1):158-168.
[25]	Guo X, Yang Y, Lu D, et al.Biofilms as a sink for antibiotic resistance genes (ARGs) in the Yangtze Estuary [J].Water Research, 2018,129:277-286.
[26]	秦荣,喻庆国,刘振亚,等.人类活动影响下的高原湿地四环素类抗生素抗性基因赋存与微生物群落共现性[J].环境科学, 2023,44(1):169-179.Qin R, Yu Q G, Liu Z Y, et al.Co-occurrence of Tetracycline Antibiotic Resistance Genes and Microbial Communities in Plateau Wetlands Under the Influence of Human Activities [J].Environmental Science, 2023,44(1):169-179.